Effects of monoamine uptake inhibitors on pain-related depression of nesting in mice

Alexander, Khadijah; Rodriguez, Taylor R.; Sarfo, Amma; Patton, T. B.; Miller, Laurence L.

doi:10.1097/fbp.0000000000000469

Cited by 5 publications

(7 citation statements)

References 46 publications

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“…In the current study we used two established analgesics to assess the validity of this procedure. Consistent with previous studies using a variety of assays of pain stimulated behavior (13,17,26,36), we found that the NSAID ketoprofen dose-dependently blocked pain-related depression of shredding. Moreover, the present study showed that ketoprofen's effects were selective for blocking pain-related depression by intraperitoneal injection of lactic acid, and did not impact depression of behavior by a pharmacological stimulus, the kappa opioid agonist U69,593.…”

Section: Discussionsupporting

confidence: 91%

“…Examples of this approach include pain-related depression of feeding (23,26), wheel-running (9, 10, 12, 29, 30), burrowing (11,(31)(32)(33)(34) and nesting. Some previous studies have used rating systems to indicate nest quality (32), but recent studies use variations of a general approach that involves assessment of the collection and consolidation of nesting material within the home cage which potentially allows for more objective measurement of the dependent variable (13)(14)(15)(16)(17)(18)(19)35).…”

Section: Discussionmentioning

confidence: 99%

“…One nesting procedure that we have used to examine the expression and treatment of pain-related depression of behavior involves distribution of nesting material across six "zones" of the home cage at the beginning of the session, and subsequent assessment of the number of zones cleared of material as the mouse consolidates the material into one zone while building the nest (13,17). In this procedure the maximum score is five zones cleared which reflects the consolidation of the nesting material into one of the six zones.…”

Section: Discussionmentioning

confidence: 99%

“…The purpose of the present study was to develop and assess the validity of a simple, low cost, procedure for examining pain-related depression of behavior in mice. This study builds on previous research showing that mouse behaviors related to nesting are sensitive to depression by pain manipulations (13,(15)(16)(17)(18)(19). Nesting behaviors are an attractive target behavior in mouse studies of pain-related depression of behavior because they are innate (i.e., do not require training), can be completed with minimal costs beyond basic animal care costs, and can potentially be completed as part a relatively rapid and unobtrusive assessment.…”

Section: Introductionmentioning

confidence: 87%

“…A number of studies have shown that pain stimuli commonly used in studies that focus on painrelated stimulation of reflexive behaviors [e.g., intra-peritoneal injection of dilute acid, paw incision, and intra-plantar injection of Complete Freund's Adjuvant (CFA)] also depress behavior in rodents. Behaviors depressed by these manipulations include conditioned behaviors such as operant responding for food and other reinforcers (6)(7)(8), and unconditioned behaviors such as feeding, wheel running, burrowing, and nesting (9)(10)(11)(12)(13). Such findings and their parallels with pain-related depression of behavior in human and veterinary patients provide evidence of the face validity of this approach to studying pain.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Ketoprofen and Morphine on Pain-Related Depression of Nestlet Shredding in Male and Female Mice

et al. 2021

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A primary goal in pain treatment is restoration of behaviors that are disrupted by pain. Measures of pain interference indicate the degree to which pain interferes with activities in pain patients, and these measures are used to evaluate the effects of analgesic drugs. As a result of the emphasis on the expression and treatment of functional impairment in clinical settings, preclinical pain researchers have attempted to develop procedures for evaluation of pain-related functional impairment in laboratory animals. The goal of the present study was to develop and validate a low cost procedure for the objective evaluation of pain-related depression of home cage behavior in mice. On test days, a 5 × 5 cm Nestlet was weighed prior to being suspended from the wire lid of the home cage of individually housed male and female ICR mice. Over the course of experimental sessions, mice removed pieces of the suspended Nestlet, and began to build a nest with the material they removed. Thus, the weight of the pieces of Nestlet that remained suspended at various time points in the session provided an indicator of the rate of this behavior. The results indicate that Nestlet shredding was stable with repeated testing, and shredding was depressed by intra-peritoneal injection of 0.32% lactic acid. The non-steroidal anti-inflammatory drug ketoprofen blocked 0.32% lactic acid-induced depression of shredding, but did not block depression of shredding by a pharmacological stimulus, the kappa opioid receptor agonist U69,593. The μ-opioid receptor agonist morphine did not block 0.32% lactic acid-induced depression of shredding when tested up to doses that depressed shredding in the absence of lactic acid. When noxious stimulus intensity was reduced by decreasing the lactic acid concentration to 0.18%, morphine was effective at blocking pain-related depression of behavior. In summary, the data from the present study support consideration of the Nestlet shredding procedure for use in studies examining mechanisms, expression, and treatment of pain-related functional impairment.

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Section: Discussionsupporting

confidence: 91%