Effects of metal elements on acid phosphatase activity in cucumber (Cucumis sativus L.) seedlings

Tabaldi, Luciane Almeri; Ruppenthal, Raquel; Cargnelutti, Denise; Morsch, Vera Maria; Pereira, Luciane Belmonte; Schetinger, Maria Rosa Chitolina

doi:10.1016/j.envexpbot.2005.10.009

Cited by 47 publications

(30 citation statements)

References 34 publications

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“…In this paper, shoot APs activity was inhibited at all Cd concentrations, whereas APs activity in root increased with Cd contamination. Interestingly, (Tabaldi et al 2007) found that Cd did not significantly alter the in vitro cucumber APs activity. We suggest that Cd can be interfering with PO 3− 4 binding sites or replacing the ions Ca 2+ or Mg 2+ in the active site of enzyme inhibiting APs activity once they are generally metalloenzymes.…”

Section: Discussionmentioning

confidence: 99%

“…3.1.3.2) Cucumber seedlings were separated into root and shoot and then were centrifuged at 43,200 g for 30 min and the resulting supernatant was used for enzyme assay. Acid phosphatases activity was determined according to (Tabaldi et al 2007) in a reaction medium consisting of 3.5 mM sodium azide, 2.5 mM calcium chloride, 100 mM citrate buffer, pH 5.5 at a final volume of 200 µL. A 20 µL aliquot of the enzyme preparation was added to the reaction mixture, except in controls, and pre-incubated for 10 min at 35…”

Section: Chlorophyll and Carotenoids Contentsmentioning

confidence: 99%

“…Cucumber is an important crop plant which was selected as a test plant species due to its sensitivity to a wide range of contaminants (Pereira et al 2006;Tabaldi et al 2007) and also due to the insufficient information available on Cd toxicity in this species. In order to get more information on the mechanisms involved in the plant tolerance to this metal, we verified the effects of Cd on plant growth, histology of the roots, chlorophyll a, chlorophyll b, total chlorophyll and carotenoids contents, δ-aminolevulinic acid dehydratase and acid phosphatase activities, soluble phosphorus (Pi) and Ca, K, Mg, Fe, Zn, and Na contents.…”

Section: Introductionmentioning

confidence: 99%

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Photosynthetic pigments content, δ-aminolevulinic acid dehydratase and acid phosphatase activities and mineral nutrients concentration in cadmium-exposed Cucumis sativus L.

et al. 2009

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In this study, the effects of cadmium chloride (CdCl2) on plant growth, histology of roots, photosynthetic pigments content, δ-aminolevulinic acid dehydratase (ALA-D; E.C. 4.2.1.24) and acid phosphatase activities (AP; E.C. 3.1.3.2), soluble phosphorus (Pi) measurement and mineral nutrients content in cucumber seedlings (Cucumis sativus L.) were investigated. Cucumber seedlings were grown in vitro in an agar-solidified substrate containing four CdCl2 treatments (0, 100, 400, and 1000 µM) for ten days. Cd was readily absorbed by seedlings and its content was greater in the roots than in the shoot. Cd reduced shoot and root length, and fresh and dry biomass of seedlings. Inhibition of root cell elongation in Cd-treated seedlings was observed by the increase of the mean radial size of cells belonging to three zones of the root tip. The highest level of Cd reduced in a similar manner chlorophyll a, chlorophyll b and total chlorophyll contents. Increasing concentrations of Cd resulted in a linear decrease in carotenoids levels of cotyledons. Interestingly, the ALA-D activity in cotyledons was inhibited only at the highest level of Cd. Root and shoot AP activities were, respectively, activated and inhibited at all CdCl2 concentrations. Root Pi concentration was increased in all Cd treatments and it was not altered in the shoot tissues. Moreover, in general, the nutrient contents were increased in the root and decreased in the shoot. Therefore, we suggest that Cd affects negatively growth, photosynthetic pigments, ALA-D and AP activities and partition of mineral nutrients in cucumber seedlings.

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Section: Discussionmentioning

confidence: 99%

Section: Chlorophyll and Carotenoids Contentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Photosynthetic pigments content, δ-aminolevulinic acid dehydratase and acid phosphatase activities and mineral nutrients concentration in cadmium-exposed Cucumis sativus L.

et al. 2009

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“…Alteration in the activity of acid phosphatase in plants has been observed under toxic conditions due to various heavy metals (Shah & Dubey 1998;Sharma & Dubey 2005). Zinc is a more potent inhibitor of acid phosphatase in cucumber than mercury (Tabaldi et al 2007). A number of metal ions are known to serve either as activators (Yupsanis et al 1993) or inhibitors (Guo & Pesacreta 1997;Tabaldi et al 2007) for acid phosphatase activity.…”

Section: Phosphorusmentioning

confidence: 99%

“…Phosphatases catalyze reactions that results in the liberation of inorganic phosphorus (Pi) from various substrates in a thermodynamically favourable process which occurs in both acidic and alkaline medium (Barret-Lennard et al 1982). Acid phosphatases (EC 3.1.3.2) catalyze nonspecific hydrolysis of inorganic phosphate from phosphate monoesters in pH ranges from 4 to 6, plays a major role in the supply and metabolism of phosphate in plants (Tabaldi et al 2007). Similarly, alkaline phosphatases (EC 3.1.3.1) have a potential role in utilization of phosphomonoesters as a source of inorganic phosphorus required for maintenance of cellular metabolism (Orhanovic & Pavela-Vrancic 2000).…”

Section: Phosphorusmentioning

confidence: 99%

Biochemical changes and adaptive strategies of plants under heavy metal stress

2011

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Heavy metal contamination of soil, aqueous waste stream and ground water causes major environmental and human health problems. Heavy metals are major environmental pollutants when they are present in high concentration in soil and show potential toxic effects on growth and development in plants. Due to unabated, indiscriminate and uncontrolled discharge of hazardous chemicals including heavy metals into the environment, plant continuously have to face various environmental constraints. In plants, seed germination is the first exchange interface with the surrounding medium and has been considered as highly sensitive to environmental changes. One of the crucial events during seed germination entails mobilization of seed reserves which is indispensable for the growth of embryonic axis. But, metabolic alterations by heavy metal exposure are known to depress the mobilization and utilization of reserve food by affecting the activity of hydrolytic enzymes. Some plants possess a range of potential mechanisms that may be involved in the detoxification of heavy metals by which they manage to survive under metal stress. High tolerance to heavy metal toxicity could rely either on reduced uptake or increase planned internal sequestration which is manifested by an interaction between a genotype and its environment. Such mechanism involves the binding of heavy metals to cell wall, immobilization, exclusion of the plasma membrane, efflux of these toxic metal ions, reduction of heavy metal transport, compartmentalization and metal chelation by tonoplast located transporters and expression of more general stress response mechanisms such as stress proteins. It is important to understand the toxicity response of plant to heavy metals so that we can utilize appropriate plant species in the rehabilitation of contaminated areas. Therefore, in the present review attempts have been made to evaluate the effects of increasing level of heavy metal in soils on the key behavior of hydrolytic and nitrogen assimilation enzymes. Additionally, it also provides a broad overview of the strategies adopted by plants against heavy metal stress.

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