Effects of lyophilization on the stability of bacteriophages against different serogroups of Shiga toxin-producing Escherichia coli

Lavenburg, Valerie M.; Liao, Yen‐Te; Salvador, A.; Hsu, Angeline L.; Harden, Leslie A.; Wu, Vivian C.H.

doi:10.1016/j.cryobiol.2020.07.012

Cited by 6 publications

(2 citation statements)

References 22 publications

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“…In addition, phage Sa45lw is stable to store at both refrigeration temperature and −80°C for 164 days. Furthermore, a previous study conducted in our lab demonstrated that Sa45lw lyophilized with cryoprotectants, such as sucrose or trehalose, was sustainable during 6-month storage at −80°C without the loss of its infectivity ( 43 ). These characteristics of Sa45lw completely accommodate the thermal and pH settings commonly used in the produce industry and for sprout production ( 44 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

et al. 2022

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Section: Discussionmentioning

confidence: 99%

Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

et al. 2022

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“…Presumably, phages may vary among themselves with respect to sensitivity to the conditions of freeze-drying, which entails the optimization of the method [ 35 ]. To standardize the process, different excipients, e.g., sucrose and trehalose, are often added to phage suspensions to significantly improve the stability of the lysates [ 28 , 36 , 37 , 38 , 39 ]. In the present study, an excipient has been added at the stage of encapsulation, and the proposed scheme for preparing dried microspheres has no equivalent in the literature on phage encapsulation.…”

Section: Discussionmentioning

confidence: 99%

Freeze-Drying of Encapsulated Bacteriophage T4 to Obtain Shelf-Stable Dry Preparations for Oral Application

Śliwka,

Skaradziński,

Dusza

et al. 2023

Pharmaceutics

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Therapeutic application of bacterial viruses (phage therapy) has in recent years been rediscovered by many scientists, as a method which may potentially replace conventional antibacterial strategies. However, one of the main problems related to phage application is the stability of bacterial viruses. Though many techniques have been used to sustain phage activity, novel tools are needed to allow long-term phage storage and application in versatile forms. In this study, we combined two well-known methods for bacteriophage immobilization. First, encapsulated phages were obtained by means of extrusion–ionic gelation, and then alginate microspheres were dried using the lyophilization process (freeze-drying). To overcome the risk of phage instability upon dehydration, the microspheres were prepared with the addition of 0.3 M mannitol. Bacteriophage-loaded microspheres were stored at room temperature for 30 days and subsequently exposed to simulated gastric fluid (SGF). The survival of encapsulated phages after drying was significantly higher in the presence of mannitol. The highest number of viable bacteriophages exceeding 4.8 log10 pfu/mL in SGF were recovered from encapsulated and freeze-dried microspheres, while phages in lyophilized lysate were completely inactivated. Although the method requires optimization, it may be a promising approach for the immobilization of bacteriophages in terms of practical application.

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Isolation, screening and characterization of phage

Zaki¹,

Mohamed²,

Dawoud³

et al. 2023

Progress in Molecular Biology and Translational Science

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Effects of lyophilization on the stability of bacteriophages against different serogroups of Shiga toxin-producing Escherichia coli

Cited by 6 publications

References 22 publications

Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

Freeze-Drying of Encapsulated Bacteriophage T4 to Obtain Shelf-Stable Dry Preparations for Oral Application

Isolation, screening and characterization of phage

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