Effects of low-molecular weight alcohols on bacterial viability

Man, Adrian; Gâz, Andrei Şerban; Mare, Anca Delia; Berța, Lavinia

doi:10.1515/rrlm-2017-0028

Cited by 17 publications

(20 citation statements)

References 21 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, this high concentration of ethanol is toxic to most cells. We found that 15% ethylene glycol or 0.1% Tween-20 were effective and non-toxic agents ( Blank et al, 1987 ; Man et al, 2017 ) for re-wetting Nafion microcosms already inoculated with bacteria or fungi. For our experiments, we therefore slowly percolated 15% ethylene glycol into the microfluidics device using a syringe pump (3 µL total volume, 0.5 µL/min) to rehydrate the hydrophobic Nafion, followed by washing with MSN medium to remove ethylene glycol (20 µL total volume, 0.5 µL/min).…”

Section: Methodsmentioning

confidence: 95%

“…This makes cryolite an attractive candidate for soil microbial ecology studies, since bacteria may be visualized even without being fluorescently labeled, obviating the need for genetic modification or staining. Cryolite also has more natural dry-down and wet-up behavior than Nafion (which requires small amounts of surfactants [15% ethylene glycol or 0.1% Tween] to be re-wet after drying; although generally non-toxic [ Blank et al, 1987 ; Man et al, 2017 ], both of these surfactants can cause cell stress [ Hallsworth et al, 2003 ; Nielsen et al, 2016 ] and can be potential sources of nutrient contamination [ Man et al, 2017 ]).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Transparent soil microcosms for live-cell imaging and non-destructive stable isotope probing of soil microorganisms

Sharma

Palatinszky

Nikolov

et al. 2020

eLife

View full text Add to dashboard Cite

Microscale processes are critically important to soil ecology and biogeochemistry yet are difficult to study due to soil’s opacity and complexity. To advance the study of soil processes, we constructed transparent soil microcosms that enable the visualization of microbes via fluorescence microscopy and the non-destructive measurement of microbial activity and carbon uptake in situ via Raman microspectroscopy. We assessed the polymer Nafion and the crystal cryolite as optically transparent soil substrates. We demonstrated that both substrates enable the growth, maintenance, and visualization of microbial cells in three dimensions over time, and are compatible with stable isotope probing using Raman. We applied this system to ascertain that after a dry-down/rewetting cycle, bacteria on and near dead fungal hyphae were more metabolically active than those far from hyphae. These data underscore the impact fungi have facilitating bacterial survival in fluctuating conditions and how these microcosms can yield insights into microscale microbial activities.

show abstract

Section: Methodsmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Transparent soil microcosms for live-cell imaging and non-destructive stable isotope probing of soil microorganisms

Sharma

Palatinszky

Nikolov

et al. 2020

eLife

View full text Add to dashboard Cite

show abstract

“…Ethyl alcohol 70% was unable to affect A. lwoffii on epoxy and ceramic surfaces; also it was not effective against B. cepacia and E. coli on ceramic surface and S. maltophilia on epoxy. Alcohols denature proteins and dissolve the lipid membranes (Man et al 2017). On the Acinetobacter haemolyticus P P P P P P P P P P P P P P P P P P Acinetobacter lwoffii P P F P P P P P P P P P P P F P P P Aeromonas hydrophila P P P P P P P P P P P P P P P P P P Aeromonas salmonicida P P P P P P P P P P P P P P P P P P Burkholderia pseudomallei P P P P P P P P P P P P P P P P P P Burkholderia cepacia P P F P P P P P P P F P P P F P P P Burkholderia gladioli P P P P P P P P P P P P P P P P P P Chryseomonas luteola P P P P P P P P P P P P P P P P P P Cronobacter sakazakii P P P P P P P F F P P P P P P P F F Delftia acidovorans P P P P P P P P P P P P P P P P P P Elizabethkingia meningoseptica P P P P P P P P P P P P P P P P P P Enterobacter cloacae P P P P P P P P P P P P P P P P P P Escherichia coli P P F P P P P P P P P P P P F P P P Leclercia adecarboxylata P P P P P P P P P P P P P P P P P P Moraxella catarrhalis P P P P P P P P P P P P P P P P P P Pantoea s p .…”

Section: Discussionmentioning

confidence: 99%

Monitoring and controlling bacteria in pharmaceutical industries water system

Selim

Saeed

Ibrahim

2020

J. Appl. Microbiol.

View full text Add to dashboard Cite

Aim This research aimed to monitor pharmaceutical water system by sampling water from all treatment stages, identify bacterial isolates from each phase and determine the most suitable methods to control them. Methods and Results Water samples were collected and examined from pharmaceutical water system in a pharmaceutical factory in Giza, Egypt during 12 months, once per month (from December 2017 to November 2018) from 15 points covering all stages of the treatment process starting from wells, pre‐treatment points; treatment points ending with purified points which are the main source of water used in all pharmaceutical process. In all, 216 water samples were collected and examined, 156 isolates were selected according to morphological characteristics. VITEK system 2 (BioMérieux) was used for identification of all isolates resulting in 24 different identified bacteria. Antibiotic assay test using disc diffusion methods were carried out using seven antibiotics from different groups. Several disinfectants were also examined for efficacy against the isolates to control micro‐organisms in water treatment stage and manufacturing area. The effect of different preservatives (parabens, acids and alcohols) in various pharmaceutical formulas was also tested on bacterial isolates, 63% of formulas were effective against all bacterial isolates. Conclusion Ciprofloxacin was the most effective antibiotic, mixture of 0·45% peracetic acid plus 2·2% of hydrogen peroxide (Minncare 1%) was maximally effective disinfectant, and Cronobacter sakazakii was the most resistant micro‐organism against 22·7% of tested preservatives. Significance and Impact of the study Controlling pharmaceutical manufacturing operation from pathogenic bacteria that affect the quality of drugs.

show abstract

“…The fact that this formulation was the less active than those with less isopropanol but containing 20 to 40% ethanol is an indication that the combination of the two alcohols is more active than using isopropanol alone. Man et al [24] after testing low molecular weight alcohols on bacterial viability concluded that n propyl alcohols were more active followed by isopropanol, n butyl and iso butyl alcohols, and all the precedent compounds were more active that ethanol. In fact alcohols can affect microorganism vitality with two main mechanism [25]; membrane lipid layer solubility and protein alcohol solubility.…”

Section: Discussionmentioning

confidence: 99%

Improvement of the Efficacy an WHO Alcohol-based Hand Rub Formulation by Reducing Isopropanol Content and Associating Syzygium aromarticum and Piper nigrum Essential Oils

Ismail

Penlap

Mbacham

et al. 2019

JALSI

View full text Add to dashboard Cite

Aim: This work was carried out in order to reduce the isopropanol contain of a World Health Organization (WHO) basic alcohol based hand rub (ABHR) formulation by substituting part of it with ethanol and essential oils. Study Design: A quasi-experimental design was used, based on WHO basic formulation modification, antimicrobial assessment followed by a panel test and challenge test of the best formulation. Place and Duration of the Study: The study was carried out in different laboratory of the University of Yaoundé 1 during March 2017 to July 2018. Methodology: This study was performed by producing different formulations nested from the WHO basic formulation and testing their antimicrobial capacity on selected strains based on the microbial percentage reduction. Following this, the best formulation was compared to two commercial products trough a panel test and later challenged with selected organism (Staphylococcus aureus SR196, Salmonella Typhi 15SA, Escherichia coli ATTC25922 and Pseudomonas aeruginosa PA01) inoculated in the product after different storage periods for one year and the antimicrobial stability assessed on the same strain for the same length of conservation. Results: The partial substitution of isopropanol with ethanol and Piper nigrum and Syzygium aromaticum essential oils proved to possess more antimicrobial properties than the original WHO formulation. The best formulation caused a 6 Log cells/ml reduction of the initial population compared to the 4 Log cells/ml of the WHO formulation. The product also proved to maintain its activity for one year and to be able to deactivate possible contaminations by Salmonella Typhi 15SA, Staphylococcus aureus NCTC10652, Escherichia coli ATCC25922, Pseudomonas aeruginosa PA01. Conclusion: The present work is a contribution to the improvement of ABHRs and could permit the reduction of hand hygiene associated infections in industries and health care facilities.

show abstract

Effects of low-molecular weight alcohols on bacterial viability

Cited by 17 publications

References 21 publications

Transparent soil microcosms for live-cell imaging and non-destructive stable isotope probing of soil microorganisms

Transparent soil microcosms for live-cell imaging and non-destructive stable isotope probing of soil microorganisms

Monitoring and controlling bacteria in pharmaceutical industries water system

Improvement of the Efficacy an WHO Alcohol-based Hand Rub Formulation by Reducing Isopropanol Content and Associating Syzygium aromarticum and Piper nigrum Essential Oils

Contact Info

Product

Resources

About