Effects of Long Chain Free Fatty Acids on Glucose-Induced Insulin Secretion in the Perfused Rat Pancreas

Mokuda, O; Sakamoto, Yoichi; Hu, Howard; Kawagoe, Rika; Shimizu, Norihiro

doi:10.1055/s-2007-1002185

Cited by 8 publications

(9 citation statements)

References 7 publications

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“…The reduction in blood glucose in groundnut oil diet-fed mice may be due to the presence of mono unsaturated fatty acids (MUFA) [36]. A reduction in plasma glucose concentration with the consumption of a MUFA-rich diet has been reported [37]. Indeed it has also been shown that when the groundnut oil was replaced with sesame oil (a much higher percentage polyunsaturated fatty acid than groundnuts) the reduction in glucose was even more significant [38].…”

Section: Resultsmentioning

confidence: 99%

Effect of dietary vegetable oil consumption on blood glucose levels, lipid profile and weight in diabetic mice: an experimental case—control study

et al. 2016

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Background: Free fatty acids have been reported to impair insulin action; Dietary fat composition has been implicated in the development of insulin resistance as well as fasting glycaemia and type 2 diabetes mellitus. This work was designed to determine the benefits of consuming vegetable oils on the management of diabetes in diabetic mice. Methods: Forty eight (48) maledb/db diabetic mice were randomly divided into eight groups of six. The first four groups were fed on chow (control), 10 % Red palm oil feed, 10 % groundnut oil feed, and 10 % coconut oil feed. The second four groups were fed similar to the first four groups but in addition were administered glibenclamide (2 mg/kg -wt i.p) daily at 8. AM.

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Section: Resultsmentioning

confidence: 99%

Effect of dietary vegetable oil consumption on blood glucose levels, lipid profile and weight in diabetic mice: an experimental case—control study

et al. 2016

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“…Second, in the fed state, this component was not needed for efficient GSIS. In considering what such a "glucose cofactor" might be, our attention turned to free fatty acids for the following reasons: (a) an elevated plasma FFA level is one of the hallmarks of the fasted state; (b) it has long been known that FFA are capable of enhancing GSIS both in vivo (17,(24)(25)(26)(27) and in vitro (9,12,16,(28)(29)(30)(31)(32)(33)(34); and (c) there is growing evidence for an interaction between glucose and fatty acid metabolism as an integral component of stimulus-secretion coupling within the ␤ cell (9-12, 16, 33-36; see below).…”

Section: Discussionmentioning

confidence: 99%

Essentiality of circulating fatty acids for glucose-stimulated insulin secretion in the fasted rat.

Stein¹,

Esser²,

Stevenson³

et al. 1996

J. Clin. Invest.

342

271

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We asked whether the well known starvation-induced impairment of glucose-stimulated insulin secretion (GSIS) seen in isolated rat pancreas preparations also applies in vivo. Accordingly, fed and 18-24-h-fasted rats were subjected to an intravenous glucose challenge followed by a hyperglycemic clamp protocol, during which the plasma-insulin concentration was measured. Surprisingly, the acute (5 min) insulin response was equally robust in the two groups. However, after infusion of the antilipolytic agent, nicotinic acid, to ensure low levels of plasma FFA before the glucose load, GSIS was essentially ablated in fasted rats, but unaffected in fed animals. Maintenance of a high plasma FFA concentration by coadministration of Intralipid plus heparin to nicotinic acid-treated rats (fed or fasted), or further elevation of the endogenous FFA level in nonnicotinic acidtreated fasted animals by infusion of etomoxir (to block hepatic fatty acid oxidation), resulted in supranormal GSIS. The in vivo findings were reproduced in studies with the perfused pancreas from fed and fasted rats in which GSIS was examined in the absence and presence of palmitate. The results establish that in the rat, the high circulating concentration of FFA that accompanies food deprivation is a sine qua non for efficient GSIS when a fast is terminated. They also serve to underscore the powerful interaction between glucose and fatty acids in normal ␤ cell function and raise the possibility that imbalances between the two fuels in vivo could have pathological consequences. ( J. Clin. Invest. 1996. 97:2728-2735.)

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“…Chronic elevation of NEFA has been associated with inhibition of GSIS. This inhibition of GSIS is unlikely to be due to permanent structural changes as reversibility in effect has been shown[29–37]. Many mechanisms have been suggested to explain the deleterious effects of chronic elevation of NEFA upon GSIS.…”

Section: Chronic Effectsmentioning

confidence: 96%

“…Mokuda et al . [32] perfused isolated rat pancreata with palmitate at varying concentrations for a period of 15 min before stimulating insulin secretion using glucose at 18 m m . They showed a dose‐dependent effect of palmitate, namely no effect upon GSIS at 0.6 m m ; an increase in GSIS at 1.0 m m ; and a more pronounced increase in GSIS at 1.4 m m .…”

Section: Nefa and Insulin Secretionmentioning

confidence: 99%

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Fatty acids and insulin secretion

Amery

Nattrass

2000

Diabetes Obesity Metabolism

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Effects of Long Chain Free Fatty Acids on Glucose-Induced Insulin Secretion in the Perfused Rat Pancreas

Cited by 8 publications

References 7 publications

Effect of dietary vegetable oil consumption on blood glucose levels, lipid profile and weight in diabetic mice: an experimental case—control study

Effect of dietary vegetable oil consumption on blood glucose levels, lipid profile and weight in diabetic mice: an experimental case—control study

Essentiality of circulating fatty acids for glucose-stimulated insulin secretion in the fasted rat.

Fatty acids and insulin secretion

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