1997
DOI: 10.1002/(sici)1099-1492(199709)10:6<257::aid-nbm471>3.0.co;2-b
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Effects of ligation and reperfusion of hepatic afferent vessels on the composition of liver cell membranes in the rat:1H- and31P-magnetic resonance spectroscopic analysis
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Cited by 9 publications
(3 citation statements)
References 23 publications
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“…This method was originally designed for the extraction of hydrophobic substances, but is also useful for extracting the low molecular weight hydrophilic organic compounds essentially without any in vitro modiˆcations, such as oxidation and hydrolysis. [24][25][26] The hydrophilic extracts were lyophilized, redissolved in 250 ml D2O (99.8 atom z D, Aldrich Chemical Co. Inc., Milwaukee, Wisconsin, USA), and placed into 5 mm specimen tubes for subsequent 1 H-NMR measurement.…”
Section: Methodsmentioning
confidence: 99%
“…This method was originally designed for the extraction of hydrophobic substances, but is also useful for extracting the low molecular weight hydrophilic organic compounds essentially without any in vitro modiˆcations, such as oxidation and hydrolysis. [24][25][26] The hydrophilic extracts were lyophilized, redissolved in 250 ml D2O (99.8 atom z D, Aldrich Chemical Co. Inc., Milwaukee, Wisconsin, USA), and placed into 5 mm specimen tubes for subsequent 1 H-NMR measurement.…”
Section: Methodsmentioning
confidence: 99%
“…AlĂ©m das enzimas descritas, outros dados podem ser utilizados para a monitorizaĂ§Ă£o da lesĂ£o do hepatĂ³cito como: metabolismo de N colesterol 18 , ATP hepĂ¡tico 19 , regeneraĂ§Ă£o de radicais livres 11 , tensões de CO 2 e O 2 no tecido hepĂ¡tico e Ă¡cido hialurĂ´nico 12,13,15 , ativaĂ§Ă£o de calpain mu 14 , a elevaĂ§Ă£o de xantinas e ATP 20 , fator de necrose tumoral e interleucina 6 (IL 6) 9 , avaliaĂ§Ă£o do fluxo hepĂ¡tico, a anĂ¡lise histolĂ³gica e a dosagem de bATP 7 . Apesar de existirem muitas opções entre as diversas substĂ¢ncias utilizadas para qualificar e quantificar a lesĂ£o da cĂ©lula hepĂ¡ti-ca optamos pela dosagem de TGO, TGP e LDH por quatro motivos principais: 1) muitos dos estudos que utilizam outras substĂ¢ncias tambĂ©m fazem dosagem de uma ou mais destas enzimas 9,12,13,15,17 ; 2) sĂ£o as substĂ¢ncias mais freqĂ¼entemente encontradas na literatura na avaliaĂ§Ă£o do dano celular; 3) sĂ£o de fĂ¡cil acesso, podendo ser dosadas em qualquer laboratĂ³rio; 4) sĂ£o, efetivamente, o que utilizamos na prĂ¡tica clĂnica no acompanhamento de nossos doentes.…”
Section: DiscussĂ£o DiscussĂ£o DiscussĂ£o DiscussĂ£o DiscussĂ£ounclassified
“…30 NMR has also been used to assess hepatic glutamine metabolism, 31 he patic energetic status after warm ischemia, 32 and to study effects of I/R on liver cell membranes. 33 A preliminary study of 1 H NMR analysis of bile after liver transplantation has been reported by Powell et al in 1990, suggesting that this technique might help to distinguish ischemia from rejection post-transplant by detecting large resonance peaks for lactate and acetate. 34 More recently, there has been a growing interest in attempting to predict graft function by analyzing bile production immediately after transplantation.…”
Section: Introductionmentioning
confidence: 99%
