Effects of Ischemia/Reperfusion on ß Cells of Pancreas and Protective Effects of Melatonin Treatment

Abstract

Oxygen free radicals are considered to be important components involved in the pathophysiological tissue alterations observed during ischemia-reperfusion (I/R). In this study, we investigated the putative protective effects of melatonin treatment on pancreatic I/R injury. Sprague Dawley male rats were subjected to 30 min of pancreatic pedicle occlusion followed by 90 min reperfusion. Melatonin (10 mg/kg. s.c) was administrated 30 min prior to ischemia or I/R application. At the end of the reperfusion periods, rats were decapitated. Pancreatic samples were taken for transmission electron microscopy. The results indicated that ischemia created b cell damage as evidenced by dilatation between the nucleus inner and outer membrane and degeneration on islets of Langerhans cells, was reversed by melatonin treatment. As melatonin administration reversed these microscopic damage, it seems likely that melatonin protects pancreatic tissue against oxidative damage.

Remote effect of kidney ischemia-reperfusion injury on pancreas: role of oxidative stress and mitochondrial apoptosis

“…Also, the treated group showed a significant decrease in lipid peroxidative enzymes ( Figures 1 A – B ) and a significant increase in antioxidant enzymes ( Figures 1 C – D ) compared to the I/R group in different periods. These results are in accordance with previous studies [ 21 , 22 ]. Abdollahzad et al .…”

“…However, the group treated with vitamin C showed significant histological improvement at different periods. These results are consistent with the findings of many previous studies [ 21 , 23 , 25 – 27 ] where the histological analysis showed that IR induced acute pancreatitis with the accumulation of inflammatory infiltrate, disruption of tissue structure, cell necrosis and hemorrhage. These conclude that the pancreas is highly susceptible to oxidative stress induced by IR injury, leading to the development of acute pancreatitis [ 21 , 23 ], and this damage can be prevented by antioxidants [ 21 ].…”

“…On the other hand, other studies have suggested that the increased lipid peroxidation may be due to ischemia that results not only in catabolization of ATP, but also in the conversion of NAD-reduced xanthine hydrogenase into the oxygen radical producing xanthine oxidase [ 4 , 21 ]. Reperfusion and reoxygenation of tissue in the presence of hypoxanthine and xanthine oxidase lead to production of aggressive oxygen radicals [ 4 , 21 ]. Also, Muñoz-Casares et al .…”

Beneficial Effects of Piperine in global cerebral ischemia/reperfusion model in irradiated rats

“…Also, I/R has been reported to induce a marked enhancement of oxidative stress in a variety of organs (Muñoz-Casares et al, 2006, Abogresha et al, 2016. Mechanistically, reperfusion and reoxygenation of tissues in the presence of hypoxanthine and xanthine oxidase lead to production of aggressive oxygen radicals (Marian et al, 2007;Yildirim et al, 2009).…”