Effects of Intracanal Irrigant MTAD Combined with Nisin at Sub-Minimum Inhibitory Concentration Levels on Enterococcus faecalis Growth and the Expression of Pathogenic Genes

Tong, Zhongchun; Huang, Lijia; Ling, Junqi; Mao, Xueli; Yang, Ning; Deng, Dongmei

doi:10.1371/journal.pone.0090235

Cited by 23 publications

(11 citation statements)

References 25 publications

(43 reference statements)

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“…In our study, Nisin was incorporated at two different concentrations 1% and 5% in the fth generation BA. The concentration of Nisin was chosen in accordance to its minimum inhibitory concentration [23]. The results of our study showed that the growth of S. mutans was signi cantly higher in group I.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antibacterial Effect of Fifth Generation Dentin Bonding Agent Incorporated With Nisin on Streptococcus Mutans

Keerthipriya

Ravikumar

Mahalaxmi

2021

Preprint

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Introduction Secondary caries is the most common cause of failure of composite restorations. This study evaluated the antibacterial efficacy of fifth generation bonding agent (BA) modified with Nisin, a polypeptide bacteriocin against Streptococcus mutans based on its growth, adherence and membrane integrity. Methods Adhesive eluents of the three experimental bonding agents, namely control BA only, bonding agent with 1 wt% Nisin (NBA 1) and 5 wt% Nisin (NBA 5) were obtained using 250 μl Brain Heart Infusion (BHI) broth. To this, 10 μl S. mutans culture was added and incubated at 37°C. Bacterial growth was estimated by changes in optical density using spectrophotometry every 20 min for 2 hours. The results were statistically analysed using one way ANOVA followed by Post Hoc test. For adherence and membrane integrity test, 10μl of BHI supplemented with 1% sucrose and 50 μl of bacterial suspension were inoculated onto the cured specimens, and incubated for 4 hours. After rinsing, 1ml of Live/Dead BacLight bacterial viability stain was added and incubated in the dark for 15 min and observed under CLSM for intact (green/live) and damaged (red/dead) bacterial membranes. Results Mean optical density was significantly higher in control group at all time intervals, with experimental groups showing concentration dependant reduction in bacterial growth. Correspondingly, the experimental groups showed higher amount of dead than live bacteria, while live bacteria were predominant in the control group. Significance Addition of an antibacterial agent Nisin in bonding agent may render the resin dentin interface more resistant to bacterial penetration, thus reducing the incidence of secondary caries.

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Section: Discussionmentioning

confidence: 99%

In Vitro Antibacterial Effect of Fifth Generation Dentin Bonding Agent Incorporated With Nisin on Streptococcus Mutans

Keerthipriya

Ravikumar

Mahalaxmi

2021

Preprint

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“…For these reasons some authors proposed the association of the antimicrobial peptide nisin in the composition of Biopure MTAD ® . They found a significantly improvement on the bactericidal activity of Biopure MTAD ® against E. faecalis [23]. Currently, the use of Biopure MTAD ® as intracanal irrigant is more restrict to final irrigation, after the use of NaOCl as main irrigant [24].…”

Section: Contact Anglementioning

confidence: 99%

Wettability of Irrigants used in Root Canal Treatment

Lopes¹

2015

Dentistry

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This study assessed the contact angle and the surface tension of different chemical solutions used in the chemomechanical preparation of root canals in order to calculate its wettability. The tested irrigants were: 2.5% sodium hypochlorite (NaOCl); 2% chlorhexidine (CHX); BioPure MTAD ® ; and 0.9% sterile physiological saline solution, as the control. A goniometer was used to measure the contact angle and the surface tension. Then the wettability of each solution was calculated using Young's equation. BioPure MTAD ® showed significantly higher contact angle (p=0.002), compared to the other substances, which did not differ among themselves. As the surface tension, BioPure MTAD ® showed significantly lower values (p=0.001). As for 2.5% sodium hypochlorite, 2% chlorhexidine, and the control, chlorhexidine presented lowest surface tension (p<0.01). BioPure MTAD ® showed the best wettability in relation to the other tested irrigant, followed by two percent of CHX and 2.5% NaOCl.

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“…152 Importantly, E. faecalis does not seem to develop resistance toward nisin. 153 Another AMP with long-confirmed antimicrobial activity against oral pathogens is human b defensin-3 (HBD-3; [154][155][156] antimicrobial activity includes S. aureus, E. coli, Fusobacterium nucleatum, Prevotella melaninogenica, Peptostreptococcus anaerobius, S. mutans, Actinomyces naeslundii, E. faecalis, and C. albicans species, for example). 157 A smaller variant of HBD-3 (15 amino acids compared to 45; HBD3-C15 158,159 ) is able to reduce fungal growth in an ex vivo model of C. albicans-infected root dentin with similar effects as chlorhexidine.…”

Section: Dental Restorative Materials Modification With Ampsmentioning

confidence: 99%