Effects of individual and combined heat and drought stress during seed filling on the oxidative metabolism and yield of chickpea (Cicer arietinum) genotypes differing in heat and drought tolerance

Abstract

Drought and heat stress are two major constraints that limit chickpea (Cicer arietinum L.) yield, particularly during seed filling. The present study aimed (i) to assess the individual and combined effects of drought and heat stress on oxidative metabolism during seed filling, and (ii) to determine any genetic variation in oxidative metabolism among genotypes differing in drought and heat tolerance and sensitivity. The plants were raised in outdoor conditions with two different times of sowing, one in November (normal-sown, temperatures <32°C−20°C (day–night) during seed filling), and the other in February (late-sown, temperatures >32°C−20°C (day–night) during seed filling). Plants were regularly irrigated to prevent any water shortage until the water treatments were applied. At both sowing times, the drought treatment was applied during seed filling (at ~75% podding) by withholding water from half of the pots until the relative leaf water content (RLWC) of leaves on the top three branches reached 42–45%, whereas leaves in the fully irrigated control plants were maintained at RLWC 85–90%. Drought-stressed plants were then rewatered and maintained under fully irrigated conditions until maturity. Several biochemical parameters were measured on the leaves and seeds at the end of the stress treatments, and seed yield and aboveground biomass were measured at maturity. Individual and combined stresses damaged membranes, and decreased PSII function and leaf chlorophyll content, more so under the combined stress treatment. The levels of oxidative molecules (malondialdehyde (MDA) and H2O2) markedly increased compared with the control plants in all stress treatments, especially across genotypes in the combined heat + drought stress treatment (increases in leaves: MDA 5.4–8.4-fold and H2O2 5.1–7.1-fold; in seeds: MDA 1.9–3.3-fold and H2O2 3.8–7.9-fold). The enzymatic and non-enzymatic antioxidants related to oxidative metabolism increased under individual stress treatments but decreased in the combined heat + drought stress treatment. Leaves had higher oxidative damage than seeds, and this likely inhibited their photosynthetic efficiency. Yields were reduced more by drought stress than by heat stress, with the lowest yields in the combined heat + drought stress treatment. Heat- and drought-tolerant genotypes suffered less damage and had higher yields than the heat- and drought-sensitive genotypes under the individual and combined stress treatments, suggesting partial cross-tolerance in these genotypes. A drought-tolerant genotype ICC8950 produced more seed yield under the combined heat + drought stress than other genotypes, and this was associated with low oxidative damage in leaves and seeds.

Transcriptome Profiling, Biochemical and Physiological Analyses Provide New Insights towards Drought Tolerance in Nicotiana tabacum L.

“…GSH content in our experiment increased with the time as drought stress prolonged in all three varieties. Awasthi et al [68] also witnessed elevated GSH content under drought stress conditions, thus triggers adaptive responses, which is in line with our findings.…”

Influence of drought and heat stress, applied independently or in combination during seed development, on qualitative and quantitative aspects of seeds of lentil (Lens culinarisMedikus) genotypes, differing in drought sensitivity

“…Moreover, upon exposure to both stresses at the same time, the lentil leaves showed extensive damage to their membranes (as indicated by high EL), chlorophyll content, and photosynthetic efficiency, which might be attributed to a significant reduction in leaf water status and stomatal conductance (Awasthi et al, 2014). Damage to membranes may occur due to the direct impacts of these stresses (Guadagno et al, 2017;Horváth et al, 2012) or intense oxidative stress generated by two stresses (Johnson et al, 2014) and has been reported in chickpea (Awasthi et al, 2017).…”

“…Moreover, upon exposure to both stresses at the same time, the lentil leaves showed extensive damage to their membranes (as indicated by high EL), chlorophyll content, and photosynthetic efficiency, which might be attributed to a significant reduction in leaf water status and stomatal conductance (Awasthi et al, ). Damage to membranes may occur due to the direct impacts of these stresses (Guadagno et al, ; Horváth et al, ) or intense oxidative stress generated by two stresses (Johnson et al, ) and has been reported in chickpea (Awasthi et al, ). Chlorophyll loss is attributed to increased photo‐oxidation (Guo, Zhou, & Zhang, ), dehydration, inhibited synthesis, or increased degradation (Tewari & Tripathy, ), which matches the observations in chickpea (Awasthi et al, ), tomato (Nankishore & Farrell, ), and rice (Kumar, Vijayalakshmi, & Vijayalakshmi, ) exposed to both stresses.…”

Drought or/and Heat-Stress Effects on Seed Filling in Food Crops: Impacts on Functional Biochemistry, Seed Yields, and Nutritional Quality

“…Substantial losses observed in crop yields under combined stress environment have been attributed to several reasons, such as metabolic changes, reductions in the period of crop developmental stages ( Reddy et al, 2004 ). Subsequent decline in light perception, decreased phenology, and perturbation of processes involved in carbon assimilation such as transpiration, photosynthesis and respiration may contribute to fewer, malformed and smaller seeds ( Barnabás et al, 2008 ; Awasthi et al, 2014 , 2017 ), which are economically futile.…”