Effects of Icariin on Atherosclerosis and Predicted Function Regulatory Network in ApoE Deficient Mice

Zhang, Yibing; Ma, Xiaoyan; Li, Xiangjun; Zhang, Tong; Qin, Meng; Ren, Liqun

doi:10.1155/2018/9424186

Cited by 13 publications

(9 citation statements)

References 67 publications

(53 reference statements)

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“…inversely correlated expression levels) would integrate a restricted list of preferential putative targets (Table 4 shows different algorithms for integrative analysis). By using a similar approach, Zhang et al have recently constructed a miRNA:mRNA regulatory network for ATH progression in icariin‐treated, high‐fat fed ApoE‐deficient mice which showed that changes in miRNA expression mainly affected the PI3K/Akt, Ras, ErbB and VEGF signalling pathways in lesions [243].…”

Section: Unveiling Rna: Rna Regulatory Network In the Progression Ofmentioning

confidence: 99%

Unveiling ncRNA regulatory axes in atherosclerosis progression

Navarro

Mallén

Cruzado

et al. 2020

Clinical & Translational Med

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Completion of the human genome sequencing project highlighted the richness of the cellular RNA world, and opened the door to the discovery of a plethora of short and long non‐coding RNAs (the dark transcriptome) with regulatory or structural potential, which shifted the balance of pathological gene alterations from coding to non‐coding RNAs. Thus, disease risk assessment currently has to also evaluate the expression of new RNAs such as small micro RNAs (miRNAs), long non‐coding RNAs (lncRNAs), circular RNAs (circRNAs), competing endogenous RNAs (ceRNAs), retrogressed elements, 3′UTRs of mRNAs, etc. We are interested in the pathogenic mechanisms of atherosclerosis (ATH) progression in patients suffering Chronic Kidney Disease, and in this review, we will focus in the role of the dark transcriptome (non‐coding RNAs) in ATH progression. We will focus in miRNAs and in the formation of regulatory axes or networks with their mRNA targets and with the lncRNAs that function as miRNA sponges or competitive inhibitors of miRNA activity. In this sense, we will pay special attention to retrogressed genomic elements, such as processed pseudogenes and Alu repeated elements, that have been recently seen to also function as miRNA sponges, as well as to the use or miRNA derivatives in gene silencing, anti‐ATH therapies. Along the review, we will discuss technical developments associated to research in lncRNAs, from sequencing technologies to databases, repositories and algorithms to predict miRNA targets, as well as new approaches to miRNA function, such as integrative or enrichment analysis and their potential to unveil RNA regulatory networks.

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Section: Unveiling Rna: Rna Regulatory Network In the Progression Ofmentioning

confidence: 99%

Unveiling ncRNA regulatory axes in atherosclerosis progression

Navarro

Mallén

Cruzado

et al. 2020

Clinical & Translational Med

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“…Epimedium is often used in traditional Chinese medicine to nourish the kidney and strengthen the bones (Kim et al, 2018). Many studies have shown that ICA could promote the proliferation and differentiation of osteoblasts, and regulate the differentiation of mesenchymal cells into osteoblasts (Li et al, 2018;Zhang et al, 2018;Zhu et al, 2018;Xu et al, 2019a). In our experiments, drug carrying was achieved by co-incubating ICA with exosomes, and the successful incorporation of ICA was detected by NTA and TEM.…”

Section: Discussionmentioning

confidence: 72%

FBS-Derived Exosomes as a Natural Nano-Scale Carrier for Icariin Promote Osteoblast Proliferation

Dong

et al. 2021

Front. Bioeng. Biotechnol.

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Icariin is a class IV drug of low solubility, permeability, and poor bioavailability. Synthetic nanomaterials have developed rapidly. However, some literatures point out that synthetic nanomaterials such as liposomes, aptamers, metal nanoparticles, and nanogels have high toxicity and are affected by the reticuloendothelial system or mononuclear phagocyte system. It is known that exosomes could be used as an ideal clinical drug delivery vehicle to avoid the above-mentioned problems to a certain extent. Studies have shown that drugs can be loaded into exosomes by passive and active loading. We used Fetal bovine serum (FBS) exosomes to carry Icariin for the first time in this experiment, FBS exosomes-Icariin (FBS EXO-ICA) more effectively promoted the proliferation of osteoblasts and bone regeneration than Icariin alone. FBS EXO-ICA could become a new nano scale drug formulation for treating diseases associated with bone loss.

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“…Data from this study suggest that treatment with AC attenuated the altered levels of oxidative stress parameters in the aortic tissue of atherosclerotic rats. Deregulation of eNOS/NO pathway enhanced atherogenesis and inflammation in the aortic tissue, but AC treatment attenuated the altered eNOS/NO signaling pathway in the aortic tissue of atherosclerotic rats [15]. Moreover, the protein expressions of NF-κB and MAPK were attenuated in the aortic tissue of AC-treated group, relative to the atherosclerosis group of rats.…”

Section: Discussionmentioning

confidence: 87%

Anhuienoside C ameliorates atherosclerosis in rats via regulation of the NFκB/eNOS/NO signaling pathway

Xu¹,

Ma²,

Qin³

et al. 2020

Trop. J. Pharm Res

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Purpose: To investigate the protective effect of anhuienoside C (AC) against high cholesterol dietinduced atherosclerosis in a rat model. Methods: Atherosclerosis was induced in rats by administration of high fat diet for 8 weeks, and AC (20 and 40 mg/kg) was administered orally. The effect of AC was determined by assessing serum lipid profiles and mediators of inflammation, as well as oxidative stress parameters in aortic tissue using enzyme-linked immunosorbent assay (ELISA). Western blot assay and reverse transcription polymerase chain reaction (RT-PCR) were used for the evaluation of protein expressions. Results: Serum levels of total cholesterol (TC), triglycerides (TGs), high density lipoprotein (HDL), low density lipoprotein (LDLP), and IL-1β, IL-18, TNF-α and NF-κB were significantly reduced in AC-treated group, relative to atherosclerotic rats (p < 0.01). Moreover, parameters of oxidative stress were attenuated in the aortic tissues of AC-treated group, when compared with atherosclerotic rats. There was significant increase in eNOS expression, and marked decrease in the expressions of MAPK and NF-kB protein in the aortic tissue homogenate of AC treated group, relative to atherosclerotic group (p <0.01). Treatment with AC attenuated the mRNA expressions of ICAM-1, VCAM-1 and MCP-1 in aortic tissue of the atherosclerotic rats. Conclusion: These results reveal that AC prevents atherosclerosis in rats by modulating the NFκB/eNOS/NO signaling pathway, and thus, can thus potentially be developed as anti-atherosclerotic agent. Keywords: Anhuienoside C, Atherosclerosis, Inflammation, Oxidative stress, Cholesterol

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Effects of Icariin on Atherosclerosis and Predicted Function Regulatory Network in ApoE Deficient Mice

Cited by 13 publications

References 67 publications

Unveiling ncRNA regulatory axes in atherosclerosis progression

Unveiling ncRNA regulatory axes in atherosclerosis progression

FBS-Derived Exosomes as a Natural Nano-Scale Carrier for Icariin Promote Osteoblast Proliferation

Anhuienoside C ameliorates atherosclerosis in rats via regulation of the NFκB/eNOS/NO signaling pathway

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