Effects of icariin on alleviating schizophrenia-like symptoms by regulating the miR-144-3p/ATP1B2/mTOR signalling pathway

Pan, Bo; Xu, Lianlian; Weng, Jianjun; Wang, Yuting; Ji, Hongyan; Han, Bing; Zhu, Xiaoyun; Liu, Yanqing

doi:10.1016/j.neulet.2022.136918

Cited by 10 publications

(8 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our research also seeks to establish whether SIRT-1, Nrf-2/HO-1, and MBP levels can be diagnostic markers for ALS. Through our research, we examined the phytoconstituent icariin, which has been utilized in the treatment of various neurodegenerative and neuropsychiatric disorders, including AD [ [67] , [68] , [69] , [70] , [71] ], Bipolar disorders [ 72 ], Ischemic Stroke [ 73 ], PD [ 14 ], and Schizophrenia [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…It may prevent oxidative injury to neurons, reduce brain inflammation, and promote neuroplasticity. These effects may be beneficial for AD, PD [ 34 ], MS, Schizophrenia [ 36 ], and Ischemic Stroke [ 37 , 38 ]. Due to its neuroprotective, anti-inflammatory, and antioxidant properties, icariin can be utilized as a neuroprotective treatment for ALS.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Icariin prevents methylmercury-induced experimental neurotoxicity: Evidence from cerebrospinal fluid, blood plasma, brain samples, and in-silico investigations

Sharma,

Mehan,

Khan

et al. 2024

Heliyon

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Icariin prevents methylmercury-induced experimental neurotoxicity: Evidence from cerebrospinal fluid, blood plasma, brain samples, and in-silico investigations

Sharma,

Mehan,

Khan

et al. 2024

Heliyon

View full text Add to dashboard Cite

“…MK-801 is a non-competitive N-methyl-D-aspartic acid receptor (NMDAR) antagonist and commonly-used to establish NMDAR hypofunction models that mimic schizophrenia ( Białoń and Wąsik, 2022 ). The drug administration was performed as described previously ( Pan et al, 2022a , b , 2023a , b ). Briefly, the rats except the controls received daily intraperitoneal injections of MK-801 (0.2 mg/kg/day, #M107, Sigma-Aldrich, St. Louis, MO, USA) between 10:00 AM and 10:30 AM for 2 weeks, while the control group were intraperitoneally injected saline (0.9%) for comparison.…”

Section: Methodsmentioning

confidence: 99%

Identification of immune-related biomarkers in peripheral blood of schizophrenia using bioinformatic methods and machine learning algorithms

Zhu,

Wang,

et al. 2023

Front. Cell. Neurosci.

Self Cite

View full text Add to dashboard Cite

Schizophrenia is a group of severe neurodevelopmental disorders. Identification of peripheral diagnostic biomarkers is an effective approach to improving diagnosis of schizophrenia. In this study, four datasets of schizophrenia patients’ blood or serum samples were downloaded from the GEO database and merged and de-batched for the analyses of differentially expressed genes (DEGs) and weighted gene co-expression network analysis (WCGNA). The WGCNA analysis showed that the cyan module, among 9 modules, was significantly related to schizophrenia, which subsequently yielded 317 schizophrenia-related key genes by comparing with the DEGs. The enrichment analyses on these key genes indicated a strong correlation with immune-related processes. The CIBERSORT algorithm was adopted to analyze immune cell infiltration, which revealed differences in eosinophils, M0 macrophages, resting mast cells, and gamma delta T cells. Furthermore, by comparing with the immune genes obtained from online databases, 95 immune-related key genes for schizophrenia were screened out. Moreover, machine learning algorithms including Random Forest, LASSO, and SVM-RFE were used to further screen immune-related hub genes of schizophrenia. Finally, CLIC3 was found as an immune-related hub gene of schizophrenia by the three machine learning algorithms. A schizophrenia rat model was established to validate CLIC3 expression and found that CLIC3 levels were reduced in the model rat plasma and brains in a brain-regional dependent manner, but can be reversed by an antipsychotic drug risperidone. In conclusion, using various bioinformatic and biological methods, this study found an immune-related hub gene of schizophrenia – CLIC3 that might be a potential diagnostic biomarker and therapeutic target for schizophrenia.

show abstract

“…Western blot experiments were conducted following previously described methods (Pan et al. 2016 , 2018 , 2022a , 2022b , 2023a , 2023b ; Pan and Deng 2019 ). Briefly, separation and concentrated gel were prepared using acrylamide (Acr), bisacrylamide (Bis), tris(hydroxymethyl)aminomethane (Tris), sodium dodecyl sulphate (SDS), N,N,N′,N′-tetramethyl ethylenediamine (TEMED), ammonium persulphate (APS), etc., according to the manufacturer’s instructions (Solarbio, Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

Preparation, characterization, and anticancer effects of an inclusion complex of coixol with β-cyclodextrin polymers

Wang,

Shen,

Chen

et al. 2023

Pharmaceutical Biology

Self Cite

View full text Add to dashboard Cite

Context Coix [ Coix lacryma-jobi L. var. mayuen (Roman.) Stapf (Poaceae)], a crop of medicinal and edible significance, contains coixol, which has demonstrated anticancer properties. However, the limited solubility of coixol restricts its potential therapeutic applications. Objective This study prepared a water-soluble coixol-β-cyclodextrin polymer (CDP) inclusion compound and evaluated its anticancer effect. Materials and methods The coixol-CDP compound was synthesized through a solvent-stirring and freeze-drying technique. Its coixol content was quantified using HPLC, and its stability was tested under various conditions. The anticancer effects of the coixol-CDP compound (4.129, 8.259, 16.518, and 33.035 mg/L for 24, 48, and 72 h) on the proliferation of non-small cell lung cancer (NSCLC) A549 cells were evaluated using an MTT assay; cell morphology was examined by Hoechst nuclear staining; apoptosis and cell cycle was detected by flow cytometry; and the expression of apoptosis-related proteins was assessed by Western blots. Results The water-soluble coixol-CDP inclusion compound was successfully prepared with an inclusion ratio of 86.6% and an inclusion yield rate of 84.1%. The coixol content of the compound was 5.63% and the compound remained stable under various conditions. Compared to coixol alone, all 24, 48, and 72 h administrations with the coixol-CDP compound exhibited lower IC 50 values (33.93 ± 2.28, 16.80 ± 1.46, and 6.93 ± 0.83 mg/L) in A549 cells; the compound also showed stronger regulatory effects on apoptosis-related proteins. Discussion and conclusions These findings offer a new perspective for the potential clinical application of Coix in NSCLC therapy and its future research.

show abstract

Effects of icariin on alleviating schizophrenia-like symptoms by regulating the miR-144-3p/ATP1B2/mTOR signalling pathway

Cited by 10 publications

References 42 publications

Icariin prevents methylmercury-induced experimental neurotoxicity: Evidence from cerebrospinal fluid, blood plasma, brain samples, and in-silico investigations

Icariin prevents methylmercury-induced experimental neurotoxicity: Evidence from cerebrospinal fluid, blood plasma, brain samples, and in-silico investigations

Identification of immune-related biomarkers in peripheral blood of schizophrenia using bioinformatic methods and machine learning algorithms

Preparation, characterization, and anticancer effects of an inclusion complex of coixol with β-cyclodextrin polymers

Contact Info

Product

Resources

About