Effects ofMentha longifoliaessential oil on ruminal and abomasal longitudinal smooth muscle in sheep

Abstract

Mentha longifolia is grown commercially for production of oils and leaves. For centuries, this plant has widely been used traditionally as a folk remedy for treatment of human and ruminant gastrointestinal complaints in the Northwest of Iran. The essential oil of the plant was extracted using hydrodistillation. In the present study a total of 12 constituents, representing 96.6% of the oil were identified by gas chromatography/mass spectrometry (GC/MS). The main compounds were 1,8-cineole (19.8%), pulegone (17.3%), caryophyllene oxide (14.8%) and isomenthone (12.4%). The effects of 0.1 to 1000 g/mL essence of Mentha longofolia (EOML) on ruminal and abomasal smooth muscle of 24 healthy sheep were assessed in vitro. In the isolated ruminal and abomasal preparations, EOML (0.1-100 g/mL) exhibited a weak spasmogenic effect followed by relaxation and complete (p < 0.05) abolition of the spontaneous contraction at the highest dose (1000 g/mL). In contrast rat ileum only showed a dose-dependent relaxation effect, and tissues preincubated with EOML decreased the acetylcholine (ACh) induced contraction. EOML (1000 g/mL) significantly inhibited the effect of ACh suggesting that the effect may be mediated via cholinergic receptors on smooth muscle. These primary data indicated that the plant contained spasmogenic and spasmolitic constituents. Moreover the results showed that the EOML profoundly alters gastrointestinal smooth muscle contraction in a dosedependent and tissue-specific manner. The results suggested that EOML has the potential for development as a prokinetic and relaxant agent that may prevent or alleviate dysfunctions of gastrointestinal motility.

Evaluation of Punica granatum Peels Extracts and its Phenolic, Alkaloid and Terpenoid Constituents Against Chemically Induced Diarrhoea in Rats

“…This activity was reverse dose dependent. The result of this experiment could be due to two types of receptors which appeared to be involved in extracts action on gastrointestinal tract: high and low affinity receptor subtypes sensitive to low and high agonist concentrations, which induced contraction and relaxation of smooth muscles respectively as found by Rouf et al (2007) and Jalilzadeh-Amin et al (2012) that the effect of aqueous peel extract is dose depended. Therefore, probably P.granatum peels extracts at low doses increased the reabsorption of NaCl and water by decreasing intestinal motility as observed by the decrease in intestinal transit by charcoal meal and by their anticholinergic and antihistaminic effects.…”

The influence of a simulated digest of an equine dietary feed additive G's formula on contractile activity of gastric smooth muscle in vitro

“…Various studies have used in vitro force transduction to investigate the impact of prokinetic pharmaceuticals on specific areas of interest within the equine GIT (Lefebvre, Callens, Colen, & Delesalle, 2017; Nieto, Maher, Maher, Stanley, Larson, & Snyder, 2013; Nieto, Morales, et al, 2013; Nieto, Rakestraw, Snyder, & Vatistas, 2000). In vitro techniques using smooth muscle from different regions of the GIT to examine the impact of different dietary components and bioactive compounds have been performed for application in species of commercial relevance (Jalilzadeh‐Amin, Maham, Dalir‐Naghadeh, & Kheiri, 2012, 2012b) or those more typically used as human models such as murines and Guinea pigs (Amira et al, 2008; Badary, Awad, Sherief, & Hamada, 2006; Budriesi et al, 2010; Gharzouli & Holzer, 2004). Equine feed additives are becoming increasingly popular as a means to maintain the general health and well‐being of companion and sport horses.…”

Pharmacological and therapeutic effects of Mentha Longifolia L. and its main constituent, menthol

“…The essence profoundly changed gastrointestinal smooth muscle contraction in a dose-dependent and tissue-specific manner. [ 51 ] Another study examined the effects of piperitenone oxide, an important chemical constituent of the essential oil of many Mentha spp. such as M. longifolia , M. spicata , M. rotundifolia , M. suaveolens and M. villosa on the guinea pig ileum.…”