Effects ofcis-resveratrol on inflammatory murine macrophages: antioxidant activity and down-regulation of inflammatory genes

Abstract

This study investigated for the first time the effects of the cis isomer of resveratrol (c-RESV) on the responses of inflammatory murine peritoneal macrophages, namely on the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) during the respiratory burst; on the biosynthesis of other mediators of inflammation such prostaglandins; and on the expression of inflammatory genes such as inducible nitric oxide synthase (NOS)-2 and inducible cyclooxygenase (COX)-2. Treatment with 1-100 microM c-RESV significantly inhibited intracellular and extracellular ROS production, and c-RESV at 10-100 microM significantly reduced RNS production. c-RESV at 1-100 microM was ineffective for scavenging superoxide radicals (O(2)(.-)), generated enzymatically by a hypoxanthine (HX)/xanthine oxidase (XO) system and/or for inhibiting XO activity. However, c-RESV at 10-100 microM decreased nicotinamide adenine dinucleotide/nicotinamide adenine dinucleotide phosphate (NADH/NADPH) oxidase activity in macrophage homogenates. c-RESV at 100 microM decreased NOS-2 and COX-2 mRNA levels in lipopolysaccharide (LPS) interferon gamma (IFN-gamma)-treated macrophages. At 10-100 microM, c-RESV also significantly inhibited NOS-2 and COX-2 protein synthesis and decreased prostaglandin E(2) (PGE(2)) production. These results indicate that c-RESV at micromolar concentrations significantly attenuates several components of the macrophage response to proinflammatory stimuli (notably, production of O(2)(.-)(-) and of the proinflammatory mediators NO(.-) and PGE(2)).

Modulation of inflammatory signaling pathways by phytochemicals in ovarian cancer

“…Similar to curcumin, inhibition of NF-jB activity by resveratrol is considered to be mediated by blocking IKK activity [38]. Resveratrol also suppresses expression of inflammatory enzymes, such as iNOS and COX-2, in macrophages and various cancer cells through the inhibition of NF-jB activity [35,40,61]. In ovarian cancer cells, resveratrol inhibits the expression of both basal level and growth factor-induced hypoxia-inducible factor-1a (HIF-1a) [8,45].…”

Effects of trans- and cis-resveratrol on Ca2+ handling in A7r5 vascular myocytes

“…Fura-2 AM was from Molecular Probes (Eugene, OR, USA). cisresveratrol was prepared at the Departamento de Química Orgánica (Universidade de Santiago de Compostela, Spain) following the method previously described (Leiro et al, 2004). All other reagents were of analytical grade.…”

Long-term resveratrol administration reduces metabolic disturbances and lowers blood pressure in obese Zucker rats

“…The positive effects of resveratrol in biological systems are wide-ranging. It acts as a cancer chemoprevention agent [16], a powerful anti-inflammatory factor and an antioxidant agent [17,18]. Its cardiovascular properties, including inhibition of platelet aggregation and promotion of vasodilation by enhancing the production of NO [19] have also been described [15,20] and recent studies have reported its beneficial effects against ageing and metabolic diseases [21][22][23][24].…”