2018
DOI: 10.14814/phy2.13647
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Abstract: The aim of this study was to determine, in vitro, the effects of X4 and R5 HIV‐1 gp120 and Tat on: (1) endothelial cell senescence and (2) endothelial cell microRNA (miR) expression. Endothelial cells were treated with media without and with: R5 gp120 (100 ng/mL), X4 gp120 (100 ng/mL), or Tat (500 ng/mL) for 24 h and stained for senescence‐associated β‐galactosidase (SA‐β‐gal). Cell expression of miR‐34a, miR‐217, and miR‐146a was determined by RT‐PCR. X4 and R5 gp120 and Tat significantly increased (~100%) ce… Show more

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Cited by 17 publications
(20 citation statements)
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References 50 publications
(131 reference statements)
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“…Thus, expression of HIV Tat in endothelial cells or in transgenic mice upregulates the expression of the microRNA miR-34a [ 32 ], a molecule that targets sirtuin 1 (SIRT1), leading to the induction of senescence [ 33 , 34 ]. Together with Tat and Nef, other HIV proteins can also increase the expression of this senescence-associated microRNA and contribute to the induction of cell senescence, as revealed after the expression of the gp120 protein from X4 and R5 HIV-1 strains in endothelial cells [ 35 ]. Analysis of senescence markers such as p16 INK4a , p53 or SA-beta-gal activity revealed that expression of Tat and Nef proteins from the simian immunodeficiency virus (SIV) in adipose tissue and human adipose stem cells also results in the induction of senescence [ 36 ].…”
Section: Virus and Senescencementioning
confidence: 99%
“…Thus, expression of HIV Tat in endothelial cells or in transgenic mice upregulates the expression of the microRNA miR-34a [ 32 ], a molecule that targets sirtuin 1 (SIRT1), leading to the induction of senescence [ 33 , 34 ]. Together with Tat and Nef, other HIV proteins can also increase the expression of this senescence-associated microRNA and contribute to the induction of cell senescence, as revealed after the expression of the gp120 protein from X4 and R5 HIV-1 strains in endothelial cells [ 35 ]. Analysis of senescence markers such as p16 INK4a , p53 or SA-beta-gal activity revealed that expression of Tat and Nef proteins from the simian immunodeficiency virus (SIV) in adipose tissue and human adipose stem cells also results in the induction of senescence [ 36 ].…”
Section: Virus and Senescencementioning
confidence: 99%
“…ET-1 mediates the reduction of vascular nitric oxide production by ECs, leading to the smooth muscle proliferation and migration, which in turn leads to arterial vasoconstriction (80), whereas EMAPII is released in response to stress such as hypoxia, mechanical strain and apoptosis (81) and acts as a pro-apoptotic factor. In addition, a recent study has shown that HIV gp120 (X4 and R5) promotes EC senescence and impairs the regulation of senescence-associated microRNAs (82). Senescent ECs develop a dysfunctional phenotype acquiring pro-inflammatory, pro-oxidant, vasoconstrictor, and prothrombotic properties (83).…”
Section: Hiv Encoded Proteins and Endothelial Dysfunctionmentioning
confidence: 99%
“…A recent report indicates that HIV Tat along with morphine induces autophagy in pulmonary ECs, suggesting a role for Tat in HIV-related pulmonary arterial hypertension in the presence of opioids (107). In addition, Tat also promotes EC senescence and dysregulation of senescence-associated microRNAs (82).…”
Section: Hiv Encoded Proteins and Endothelial Dysfunctionmentioning
confidence: 99%
“…These studies support the hypothesis that HIV proteins could be present in AT and could have a deleterious effect on nearby noninfected ASC and adipocytes by a "bystander effect". Previous studies have reported that Tat and Nef can induce cellular senescence and dysfunction in endothelial cells [33] and in bone marrow MSCs [19]. Accordingly, we found that in vitro incubation with the HIV proteins Tat and Nef lowered ASCs' proliferative capacity and resulted in higher levels of several senescence biomarkers, including SA-beta-galactosidase activity, cell cycle arrest proteins (p16 and phosphorylated p53), and SASP proteins (secreted IL-6 and IL-8).…”
Section: Discussionmentioning
confidence: 53%