Effects of High-Grain Diet with Buffering Agent on the Milk Protein Synthesis in Lactating Goats

Abstract

13Feeding of straw as main roughage with numerous high-grain diets improves the 14 performance of ruminants but it can easily lead to subacute ruminal acidosis.In recent 15 years, buffering agent is applied to prevent the acid poisoning of ruminants and improve 16 the production performance of ruminants in animal husbandry. it is necessary to 17 126 Nanjing, China) and the MyiQ2 Real-time PCR system (Bio-Rad, USA) with the 127 following cycling conditions: 95°C for 2 min, 40 cycles of 95°C for 15 sec and 60°C 128 for 30 sec. Glyeraldehyde 3-phosphate dehydrogenase (GAPDH) served as reference 129 for normalization. 130 Protein extraction from mammary tissues 131 Protein extraction from mammary tissues was performed according the method 132 described by Duanmu et al (12). The mammary tissue samples of all goats in BG in 133 equal quality were mixed and washed three times with ice-cold saline containing 1 mM 134