Effects of High Dietary Carbohydrate and Lipid Intake on the Lifespan of C. elegans

Franco-Juárez, Berenice; Gómez-Manzo, Saúl; Hernández-Ochoa, Beatriz; Cárdenas-Rodríguez, Noemí; Arreguín-Espinosa, Roberto; Cruz, Verónica Pérez de la; Ortega-Cuellar, Daniel

doi:10.3390/cells10092359

Cited by 10 publications

(5 citation statements)

References 159 publications

(169 reference statements)

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“…Lipids executed many essential functions, for example, they were efficient energy storage molecules, formed a double-layer membrane structure, and played as signaling molecules [ 48 ]. However, incorrectly lipid homeostasis would develop dysregulated neurological and developmental consequences [ 37 ]; moreover, lipid overload could reduce lifespan of C. elegans [ 37 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans

Zhang

Zhou

et al. 2022

Oxidative Medicine and Cellular Longevity

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Nowadays, human beings are exposed to Cu in varieties of environmental mediums, resulting in health risks needing urgent attention. Our research found that Cu shortened lifespan and induced aging-related phenotypes of Caenorhabditis elegans (C. elegans). Transcriptomics data showed differential expression genes induced by Cu were mainly involved in regulation of metabolism and longevity, especially in fatty acid metabolism. Quantitative detection of free fatty acid by GC/MS further found that Cu upregulated free fatty acids of C. elegans. A mechanism study confirmed that Cu promoted the fat accumulation in nematodes, which was owing to disorder of fatty acid desaturase and CoA synthetase, endoplasmic reticulum unfolded protein response (UPRER), mitochondrial membrane potential, and unfolded protein response (UPRmt). In addition, Cu activated oxidative stress and prevented DAF-16 translocating into nuclear with a concomitant reduction in the expression of environmental stress-related genes. Taken together, the research suggested that Cu promoted aging and induced fat deposition and oxidative damage.

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Section: Discussionmentioning

confidence: 99%

Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans

Zhang

Zhou

et al. 2022

Oxidative Medicine and Cellular Longevity

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show abstract

“…Further evaluations were then performed in vivo on C. elegans . In contrast to in vivo rodent models, the short lifespan of the nematode makes it an ideal model to study the detrimental effects of high glucose exposure ( 76 ). The insulin signaling pathway is conserved across diverse metazoan.…”

Section: Discussionmentioning

confidence: 99%

Anti-hyperglycemic contours of Madhugrit are robustly translated in the Caenorhabditis elegans model of lipid accumulation by regulating oxidative stress and inflammatory response

Balkrishna

Gohel²,

Pathak³

et al. 2022

Front. Endocrinol.

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BackgroundThe prevalence of diabetes has considerably increased in recent years. In the long run, use of dual therapy of anti-diabetic agents becomes mandatory to attain euglycemia. Also, the incidences of diabetes-related co-morbidities have warranted the search for new therapeutic approaches for the management of the disease. Traditional herbo-mineral, anti-diabetic agents like Madhugrit are often prescribed to mitigate diabetes and related complications. The present study aimed to thoroughly characterize the pharmacological applications of Madhugrit.MethodsPhytometabolite characterization of Madhugrit was performed by ultra-high performance liquid chromatography. Evaluation of cell viability, α-amylase inhibition, glucose uptake, inflammation, and wound healing was performed by in vitro model systems using AR42J, L6, THP1, HaCaT cells, and reporter cell lines namely NF-κB, TNF-α, and IL-1β. The formation of advanced glycation end products was determined by cell-free assay. In addition, the therapeutic potential of Madhugrit was also analyzed in the in vivo Caenorhabditis elegans model system. Parameters like brood size, % curling, glucose and triglyceride accumulation, lipid deposition, ROS generation, and lipid peroxidation were determined under hyperglycemic conditions induced by the addition of supraphysiological glucose levels.ResultsMadhugrit treatment significantly reduced the α-amylase release, enhanced glucose uptake, decreased AGEs formation, reduced differentiation of monocyte to macrophage, lowered the pro-inflammatory cytokine release, and enhanced wound healing in the in vitro hyperglycemic (glucose; 25 mM) conditions. In C. elegans stimulated with 100 mM glucose, Madhugrit (30 µg/ml) treatment normalized brood size, reduced curling behavior, decreased accumulation of glucose, triglycerides, and lowered oxidative stress.ConclusionsMadhugrit showed multimodal approaches in combating hyperglycemia and related complications due to the presence of anti-diabetic, anti-inflammatory, anti-oxidant, wound healing, and lipid-lowering phytoconstituents in its arsenal. The study warrants the translational use of Madhugrit as an effective medicine for diabetes and associated co-morbidities.

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“…In this latter case, genetically modified strains are commonly used as a powerful tool to highlight the role of specific genes in pathways regulations, but changes of diets are also tested, particularly in the case of Drosophila (High sucrose diet) 89 and for C. elegans (High sugar – high lipid diet, starch supplemented diet). 90 , 91 However, even if microbiota may be involved in energy homeostasis regulation in both Drosophila and rodents, it should be noted that underlying regulatory mechanisms may be very different as shown in axenic fruit flies that are capable to store more energy compared to their conventional counterparts, 92 whereas GF rodents are lean and resistant to high-fat diets (HFD) relative to conventional rodents. 93 Still, even if it remains necessary to be cautious in the translation of the results to humans, the use of Drosophila and C. elegans is expanding to screen probiotic bacteria that could be efficient in humans on specific immune-metabolic health outcomes and traits conserved in these models (longevity, specific metabolic pathways, lipids storage.…”

Section: Part C: Models Of Determining Casual Role Of Gut Microbiota ...mentioning

confidence: 99%

Approaches to discern if microbiome associations reflect causation in metabolic and immune disorders

et al. 2022

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Our understanding of microorganisms residing within our gut and their roles in the host metabolism and immunity advanced greatly over the past 20 years. Currently, microbiome studies are shifting from association and correlation studies to studies demonstrating causality of identified microbiome signatures and identification of molecular mechanisms underlying these interactions. This transformation is crucial for the efficient translation into clinical application and development of targeted strategies to beneficially modulate the intestinal microbiota. As mechanistic studies are still quite challenging to perform in humans, the causal role of microbiota is frequently evaluated in animal models that need to be appropriately selected. Here, we provide a comprehensive overview on approaches that can be applied in addressing causality of host-microbe interactions in five major animal model organisms ( Caenorhabditis elegans, Drosophila melanogaster , zebrafish, rodents, and pigs). We particularly focused on discussing methods available for studying the causality ranging from the usage of gut microbiota transfer, diverse models of metabolic and immune perturbations involving nutritional and chemical factors, gene modifications and surgically induced models, metabolite profiling up to culture-based approached. Furthermore, we addressed the impact of the gut morphology, physiology as well as diet on the microbiota composition in various models and resulting species specificities. Finally, we conclude this review with the discussion on models that can be applied to study the causal role of the gut microbiota in the context of metabolic syndrome and host immunity. We hope this review will facilitate important considerations for appropriate animal model selection.

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Effects of High Dietary Carbohydrate and Lipid Intake on the Lifespan of C. elegans

Cited by 10 publications

References 159 publications

Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans

Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans

Anti-hyperglycemic contours of Madhugrit are robustly translated in the Caenorhabditis elegans model of lipid accumulation by regulating oxidative stress and inflammatory response

Approaches to discern if microbiome associations reflect causation in metabolic and immune disorders

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