Effects of Hemicellulose and Lignin on Enzymatic Hydrolysis of Cellulose from Dairy Manure

Liao, Wei; Wen, Zhiyou; Hurley, Sharon; Liu, Yan; Liu, Chuanbin; Chen, Shulin

doi:10.1007/978-1-59259-991-2_86

Cited by 7 publications

(10 citation statements)

References 15 publications

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“…The enzyme loading increase also favored the enzymatic hydrolysis of cellulose from hardwood (Gan et al 2003), softwood (Pan et al 2005), willow (Eklund et al 1990), sugarcane bagasse (Manonmani and Sreekantiah 1987), rice husks (Yáñez et al 2006), rice straw (Kaur et al 1998;Vlasenko et al 1997), and dairy manure fibers (Liao et al 2005). Nevertheless, the enzyme dosage required to achieve a complete conversion of cellulose into glucose vary to each raw material.…”

Section: Comparison Of Cellulose Conversion From Bsg With Other Lignomentioning

confidence: 98%

“…Currently, enzymatic hydrolysis has attracted increasing attention as an alternative to acid hydrolysis because the process is highly specific, can be performed under milder reaction conditions (pH around 5 and temperature less than 50°C) with lower energy consumption and lower environmental impact. In addition, it does not present corrosion problems, and gives high yield of pure glucose with low formation of by-products that is favorable for the hydrolysate use in bioconversion processes (Frennesson et al 1985;Liao et al 2005;Martín et al 2002;Wen et al 2004). Due to these advantages, the enzymatic hydrolysis has been extensively studied in several lignocellulosic substrates, including rice straw (Kaur et al 1998;Vlasenko et al 1997), rice hull (Sharma et al 2001), corn stover (Varga et al 2002), corncob (Chen et al 2007), dairy manure (Liao et al 2005), softwoods (Pan et al 2005;Wu et al 1999), and sugarcane bagasse (Martín et al 2002).…”

Section: Introductionmentioning

confidence: 97%

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The effect of agitation speed, enzyme loading and substrate concentration on enzymatic hydrolysis of cellulose from brewer’s spent grain

et al. 2008

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Brewer's spent grain components (cellulose, hemicellulose and lignin) were fractionated in a two-step chemical pretreatment process using dilute sulfuric acid and sodium hydroxide solutions. The cellulose pulp produced was hydrolyzed with a cellulolytic complex, Celluclast 1.5 L, at 45°C to convert the cellulose into glucose. Several conditions were examined: agitation speed (100, 150 and 200 rpm), enzyme loading (5, 25 and 45 FPU/g substrate), and substrate concentration (2, 5 and 8% w/v), according to a 2 3 full factorial design aiming to maximize the glucose yield. The obtained results were interpreted by analysis of variance and response surface methodology. The optimal conditions for enzymatic hydrolysis of brewer's spent grain were identified as 100 rpm, 45 FPU/g and 2% w/v substrate. Under these conditions, a glucose yield of 93.1% and a cellulose conversion (into glucose and cellobiose) of 99.4% was achieved. The easiness of glucose release from BSG makes this substrate a raw material with great potential to be used in bioconversion processes.

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Section: Comparison Of Cellulose Conversion From Bsg With Other Lignomentioning

confidence: 98%

Section: Introductionmentioning

confidence: 97%

The effect of agitation speed, enzyme loading and substrate concentration on enzymatic hydrolysis of cellulose from brewer’s spent grain

et al. 2008

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“…Related work has shown how significant a role xylan ''masking'' can play in hindering cellulose hydrolysis, and how beneficial the addition of xylanase can be in enhancing fiber swelling and overall cellulose accessibility (Hu et al, 2011;Liao et al, 2005).…”

Section: Synergism Between Swollenin and Hydrolytic Cellulase And Xylmentioning

confidence: 99%

Swollenin aids in the amorphogenesis step during the enzymatic hydrolysis of pretreated biomass

Gourlay

Arantes

et al. 2013

Bioresource Technology

114

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A key limitation in the overall hydrolysis process is the restricted access that the hydrolytic enzymes have due to the macro-and-micro structure of cellulose and its association with hemicellulose and lignin. Previous work has shown that several non-hydrolytic proteins can disrupt cellulose structure and boost the activity of hydrolytic enzymes when purer forms of cellulose are used. In the work reported here, Swollenin primarily disrupted the hemicellulosic fraction of pretreated corn stover, resulting in the solubilisation of monomeric and oligomeric sugars. Although Swollenin showed little synergism when combined with the cellulase monocomponents exoglucanase (CEL7A) and endoglucanase (CEL5A), it showed pronounced synergism with xylanase monocomponents Xylanase GH10 and Xylanase GH11, resulting in the release of significantly more xylose (>300%). It appears that Swollenin plays a role in amorphogenesis and that its primary action is enhancing access to the hemicellulose fraction that limits or masks accessibility to the cellulose component of lignocellulosic substrates.

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“…Being similar to lignin, hemicelluloses also play a role as physical barriers to limit the accessibility of cellulase. It is evident that the removal of hemicellulose by acid hydrolysis or steam pre‐treatment could increase biomass enzymatic digestibility 93–96. Xylan content has been found to influence both the enzyme requirements for hydrolysis and the recovery of sugars during the pre‐treatment process 97.…”

Section: Effects Of Chemical and Physical Features Of Biomass On Its mentioning

confidence: 99%

Biomass recalcitrance. Part I: the chemical compositions and physical structures affecting the enzymatic hydrolysis of lignocellulose

Zhao

Zhang

Liu

2012

Biofuels Bioprod Bioref

801

492

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Lignocellulosic biomass is recalcitrant to biodegradation due to the rigid and compact structure of plant cell wall. The recalcitrance of biomass is mainly constructed by its chemical compositions that build a spatial network as a protective bulwark. Generally, the factors affecting the accessibility of biomass cellulose can be divided into direct and indirect factors. The direct factors refer to the accessible surface area, and the indirect factors include biomass structure‐relevant factors (pore size and volume, particle size, and specific surface area), chemical compositions (lignin, hemicelluloses, and acetyl group), and cellulose structure‐relevant factors (cellulose crystallinity and degree of polymerization). Pre‐treatment is actually the process to alter indirect factors and improve direct factors thus enhancing the accessibility of cellulose. In this review, we summarize the effects of chemical compositions and physical structures on the enzymatic digestibility of lignocellulosic biomass. We suggest that future work should be focused on but not limited to the molecular mechanisms of biomass recalcitrance by investigating the microscale and nanoscale features as well as hydrogen bonds network of lignocellulosic biomass. © 2012 Society of Chemical Industry and John Wiley & Sons, Ltd

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Effects of Hemicellulose and Lignin on Enzymatic Hydrolysis of Cellulose from Dairy Manure

Cited by 7 publications

References 15 publications

The effect of agitation speed, enzyme loading and substrate concentration on enzymatic hydrolysis of cellulose from brewer’s spent grain

The effect of agitation speed, enzyme loading and substrate concentration on enzymatic hydrolysis of cellulose from brewer’s spent grain

Swollenin aids in the amorphogenesis step during the enzymatic hydrolysis of pretreated biomass

Biomass recalcitrance. Part I: the chemical compositions and physical structures affecting the enzymatic hydrolysis of lignocellulose

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