Effects of Genotype and Picloram Concentrations on Callus Induction and Plant Regeneration from Immature Inflorescence of Spring Barley Cultivars (<i>Hordeum Vulgare</i>L.)

Şener, Okan; Can, Ersin; Arslan, Merve; Celis, Nydia

doi:10.1080/13102818.2008.10817578

Cited by 13 publications

(11 citation statements)

References 21 publications

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“…The amount of this auxin required to induce somatic embryogenesis was shown to be highly variable among species. Picloram has also been effective in inducing somatic embryogenesis in other species at different concentrations: 7.5 mg/L in springer barley (Sener et al 2008), 6 mg/L in tulip (Ptak and Bach 2007), 2-4 mg/L in peach palm (Steinmacher et al 2007), 2.4 mg/L in macaw palm (Moura et al 2009), and 60 mg/L oil palm (ScherwinskiPereira et al 2010). In a study comparing 2,4-D and picloram (2 mg/L) for induction of somatic embryogenesis in Tritordeum, wheat, and barley inflorescences, embryogenesis was obtained only in medium supplemented with picloram in the latter species.…”

Section: Discussionmentioning

confidence: 97%

Optimization of somatic embryogenesis and in vitro plant regeneration of Urochloa species using picloram

Takamori

Neto

Vieira

et al. 2015

In Vitro Cell.Dev.Biol.-Plant

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The establishment of an efficient methodology for regeneration in Urochloa spp. via somatic embryogenesis is an essential component of genetic engineering technology. The aim of this study was to evaluate different concentrations of growth regulators on callus induction, somatic embryogenesis, and plant regeneration of Urochloa spp. using mature seeds as the initial explant. Firstly, different concentrations (1, 2, 4, and 8 mg/L) of 2,4-D or picloram were tested using U. brizantha cv. Marandu. In a second step, the above concentrations of picloram were also used to evaluate its effect on the morphogenetic potential in different genotypes: U. brizantha cvs. Xaraés and Piatã, U. decumbens cv. Basilisk, U. humidicola cv. Llanero, and U. ruziziensis cv. Ruziziensis. There was no significant difference between concentrations of 2,4-D or picloram for the production of primary calluses in U. brizantha cv. Marandu, ranging from 58.5 to 69.2%. However, embryogenic calluses induced under picloram showed higher percentage of shoot formation after transferring to MS medium with 2 mg/L BA. A higher number of shoots were produced in mature seed cultures of U. decumbens cv. Basilisk and U. brizantha cvs. Marandu and Xaraés supplemented with 1 mg/L picloram. No albino plants were regenerated using picloram. The successful regeneration of green and morphologically normal plants opens the possibility of using this protocol to obtain transgenic plants in Urochloa spp.

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Section: Discussionmentioning

confidence: 97%

Optimization of somatic embryogenesis and in vitro plant regeneration of Urochloa species using picloram

Takamori

Neto

Vieira

et al. 2015

In Vitro Cell.Dev.Biol.-Plant

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“…Regarding specifically the role of sugars in organogenesis, they can act in the regulation of gene expression through specific signaling, especially sucrose, during transcription and translation of mRNA (Messenger ribonucleic acid) in plants, including also the induction of genes, protein production, expression and repression of genes (Farrar et al 2000;Coruzzi and Zhou 2001;Koch 2004;Şener et al 2008;Smet et al 2009). …”

Section: Resultsmentioning

confidence: 99%

In vitro morphogenic response of leaf sheath of Phyllostachys bambusoides

Komatsu

Batagin-Piotto

Brondani

et al. 2011

Journal of Forestry Research

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Nodal segments from secondary branches of saplings of Phyllostachys bambusoides were inoculated in MS medium to assess the in vitro morphogenic response of leaf sheath through the induction to callogenesis by Picloram (4-amino-3,5,6-trichloropicolinic acid) at different concentrations of carbohydrate under the same conditions with presence or absence of luminosity. In our experiment, secondary explants were kept in MS medium containing 8.0 mg⋅L -1 of Picloram for the callus formation. Calluses were transferred in MS medium supplemented with sucrose, fructose and glucose (control, 2%, 4% and 6%). Results show that Picloram induced the callogenesis in leaf sheath. The secondary embryogenesis was formed in yellow-globular callus. The sucrose as carbohydrate source in the absence of light was more efficient to induce rhizogenesis. Glucose was more efficiency in the presence of light. Callogenic induction and further embryogenesis evidenced the competence and determination of leaf sheath cells.

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“…Nevertheless, information about picloran use in callus induction experiments has been controversial. Sener et al (2008) affirmed that the picloran effect on calli induction is highly genotype dependant and produced only albino barley plantlets from picloran-induced callus. Mendonza & Kaeppler (2002) observed that picloran significantly enhanced callus growth from mature embryos of wheat; however, the embryogenic response and plant regeneration were low.…”

Section: Calli Induction In Dark Room Followed By Light Conditions Usmentioning

confidence: 90%

“…Fuego on Schenk and Hildebrant (SH) medium supplemented with 2 mg.L -1 picloram from nodal segments. Can et al (2008) obtained 18% of callus induction in crested wheatgrass (Agropyron cristatum (L.) Gaertn) when using 6 mg.L -1 , but the response was media dependant. Sonyia et al (2001), on the other hand, easily induced regenerative calli in tomato by using a combination of picloram and benzyladenine (BA).…”

Section: Calli Induction In Dark Room Followed By Light Conditions Usmentioning

confidence: 99%

“…calli and cell suspension lines is of great interest since cells in suspension tend to be very prolific and can reach logarithmic phases by culture media exchanges and stimulations with different agents (Grosser & Gmitter, 1990). Darkness and some growth regulators such as 4-amino-3,5,6-tricloropicolinic acid (Picloran), giberellic acid (GA 3 ), Kinetin, 2,4-dichlorophenoxyacetic acid (2,4-D), 2-isopenteniladenine (2iP), and benzyladenine (BA) have been used to induce calli formation in several species (Can et al, 2008;Costa, 2007;Mendonza & Kaeppler, 2002;Khaleghi et al, 2008;Nakajima et al, 1990;Sener et al, 2008;Sonyia et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Calogênese, curva de crescimento de calos e formação de suspensões celulares de Lavandula

Bona

Santos²,

Biasi

2012

Agraria

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The cultivation of calli and cell suspension lines of aromatic plants is important because they are considered to be factories of active compounds such as, for instance, the rosmarinic acid found in Lavandula species. L. angustifolia is considered the most important species because it is regarded to be practically free from camphor, differently from other species in the genus, however, it is less productive than L. dentata, which is very productive and easily asexually propagated, but presents higher content of camphor. Information about calli and cell suspensions induction and maintenance for Lavandula species, especially the latter, is scarce. Not only that, but once callus formation is induced, it is necessary to identify the growth dynamic of the cells in order to maintain healthy, logarithmically phased growing cells for future elicitation. The goals of this work were to induce calli formation, evaluate growth curve and produce cell suspensions of Lavandula angustifolia and Lavandula dentata, two Lavandula species of great economical importance. Apical and lateral buds of L. angustifolia and L. dentata were placed on MS + 1 mg.L -1 2.4-D or picloran + 0.5 mg.L -1 BAP or 1 mg.L -1 2.4-D + 0.5 mg.L -1 GA 3 , in the presence of light or in the dark. Calli were obtained only from L. angustifolia, under light, with the combination of 2.4-D + GA 3 . The growth curve of L. angusifolia calli indicated the need for media exchange every three weeks. A blue pigment which may be interesting as a dye in the food industry was present in the media. Cells suspensions were formed.Key words: Growth regulator, lavender, micropropagation, tissue culture. Calogênese, curva de crescimento de calos e formação de suspensões celulares de Lavandula RESUMO O cultivo de calos e suspensões celulares de plantas aromáticas é importante porque estes são considerados fábricas de compostos ativos como, por exemplo, o ácido rosmarínico encontrado em espécies de Lavandula. L. angustifolia é considerada a espécie mais importante por ser praticamente livre de cânfora, todavia, é menos produtiva que L. dentata, que é facilmente propagada vegetativamente, mas apresenta alto teor de cânfora. Informação sobre indução e manutenção de calos e suspensões celulares de espécies de Lavandula, especialmente a última, é escassa. Não somente isto, mas uma vez que a formação de calos é induzida, é necessário identificar o crescimento dinâmico das células para mantê-las com crescimento saudável, em fase logarítmica para futuras elicitações. Os alvos deste trabalho foram indução de calos, avaliação de curva de crescimento e formação de suspensões celulares de Lavandula angustifolia e Lavandula dentata, duas espécies de Lavandula de grande importância econômica. Gemas de L. angustifolia e L. dentata foram colocadas em MS + 1 mg.L -1 2,4-D ou picloran + 0,5 mg.L -1 BAP ou 1 mg.L -1 2,4-D + 0,5 mg.L -1 GA 3 , em condições de luz, ou no escuro. Foram obtidos calos de L. angustifolia em condições de luz, sendo 2,4-D + GA 3 a melhor combinação. A curva de cr...

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Effects of Genotype and Picloram Concentrations on Callus Induction and Plant Regeneration from Immature Inflorescence of Spring Barley Cultivars (Hordeum VulgareL.)

Cited by 13 publications

References 21 publications

Optimization of somatic embryogenesis and in vitro plant regeneration of Urochloa species using picloram

Optimization of somatic embryogenesis and in vitro plant regeneration of Urochloa species using picloram

In vitro morphogenic response of leaf sheath of Phyllostachys bambusoides

Calogênese, curva de crescimento de calos e formação de suspensões celulares de Lavandula

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