Effects of G-Rh2 on mast cell-mediated anaphylaxis via AKT-Nrf2/NF-κB and MAPK-Nrf2/NF-κB pathways

Xu, Chang; Li, Liangchang; Wang, Chongyang; Jiang, Jingzhi; Zhu, Lianhua; Jin, Songqing; Jin, Zhehu; Lee, Jun Young; Li, Guanhao; Yan, Guanghai

doi:10.1016/j.jgr.2021.10.001

Cited by 25 publications

(14 citation statements)

References 48 publications

(47 reference statements)

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“…As shown, Ca-EE effectively downregulated Lyn kinase phosphorylation, which is concluded to be a factor in its antiallergic activities. Similarly, the phosphorylation of its known downstream kinases such as Syk, PKCδ, and AKT [ 52 , 53 ] was strongly reduced by Ca-EE ( Figure 3 b–d). Nonetheless, since we did not confirm the actual role of this extract in the activation of Lyn and Syk with their antagonists or their genes; currently, we are unable to specify real target of Ca-EE.…”

Section: Discussionmentioning

confidence: 95%

Antiallergic Effects of Callerya atropurpurea Extract In Vitro and in an In Vivo Atopic Dermatitis Model

Choi

Lee

Manilack

et al. 2023

Plants

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(1) Background: Callerya atropurpurea is found in Laos, Thailand, and Vietnam. Although the anti-inflammatory action of C. atropurpurea has been investigated, the functions of this plant in allergic responses are not understood. Here, we explored the antiallergic mechanism of C. atropurpurea ethanol extract (Ca-EE) using in vitro assays and an in vivo atopic model. (2) Methods: The constituents of Ca-EE were analyzed using GC/MS. Inhibition of lipoxygenase and β-hexosaminidase activity was examined, and the expression of inflammatory genes was measured by quantitative real-time PCR. The regulatory roles of Ca-EE in IgE/FcεRI signaling were examined by Western blotting. The DNCB-induced atopic dermatitis mouse model was performed with histological analysis. (3) Results: Ca-EE comprised cis-raphasatin, lupeol, some sugars, and fatty acids. In RBL-2H3 cells, treatment with Ca-EE significantly reduced the activities of lipoxygenase and β-hexosaminidase, as well as cytokine gene expression. IgE-mediated signaling was downregulated by blocking Lyn kinases. Moreover, Ca-EE effectively inhibited allergic symptoms in the DNCB-induced atopic dermatitis model without toxicity. (4) Conclusions: Ca-EE displayed antiallergic activities through regulating IgE/Lyn signaling in RBL-2H3 cells and a contact dermatitis model. These results indicate that Ca-EE could be effective for allergic disease treatment.

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Section: Discussionmentioning

confidence: 95%

Antiallergic Effects of Callerya atropurpurea Extract In Vitro and in an In Vivo Atopic Dermatitis Model

Choi

Lee

Manilack

et al. 2023

Plants

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“…Meanwhile, we analyzed the relationship between NRF2 and immune cells and found that NRF2 was related to MCs. Other studies indicated that NRF2 could activate MCs, IgG/Fc ε RI promoted the phosphorylation of Lyn and activated Syk/PI3K, LAT/p38, and LAT/Raf-1/ERK1/2 pathways, and the AKT-Nrf2 and p38MAPK-Nrf2 signal pathways play an important role in hypersensitivity induced by MCs [ 48 , 49 ]. Hence, we analyzed the MCs in EC tissue and found that MCs were irrelevant to OS.…”

Section: Discussionmentioning

confidence: 99%

Activated Mast Cells Combined with NRF2 Predict Prognosis for Esophageal Cancer

Guo

Shen

Sun

et al. 2023

Journal of Oncology

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Background. Esophageal cancer (EC) had the sixth-highest mortality rate of all cancers due to its poor prognosis. Immune cells and mutation genes influenced the prognosis of EC, but their combined effect on predicting EC prognosis was unknown. In this study, we comprehensively analyzed the immune cell infiltration (ICI) and mutation genes and their combined effects for predicting prognosis in EC. Methods. The CIBERSORT and ESTIMATE algorithms were used to analyse the ICI scape based on the TCGA and GEO databases. EC tissues and pathologic sections from Huai’an, China, were used to verify the key immune cells and mutation genes and their interactions. Results. Stromal/immune score patterns and ICI/gene had no statistical significance in overall survival (OS) ( p > 0.05 ). The combination of ICI and tumor mutation burden (TMB) showed that the high TMB and high ICI score group had the shortest OS ( p = 0.004 ). We recognized that the key mutation gene NRF2 was significantly different in the high/low ICI score subgroups ( p = 0.002 ) and positivity with mast cells (MCs) ( p < 0.05 ). Through experimental validation, we found that the MCs and activated mast cells (AC-MCs) were more infiltration in stage II/III ( p = 0.032 ; p = 0.013 ) of EC patients and that NRF2 expression was upregulated in EC ( p = 0.045 ). AC-MCs combined with NRF2 had a poor prognosis, according to survival analysis ( p = 0.056 ) and interactive analysis ( p = 0.032 ). Conclusions. We presume that NRF2 combined with AC-MCs could be a marker to predict prognosis and could influence immunotherapy through regulating PD-L1 in the EC.

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“…189 In an OVA-induced asthma mouse model, Rh2 inhibited serum IgE production and activation of Syk and Lyn in lung tissue. 187 Rh2 inhibited mast cell-induced allergic inflammation via the AKT-Nrf2/NF-κB and p38 MAPK-Nrf2/NF-κB pathways. 187 These studies showed great promise for the use of ginsenosides in the preventive treatment of allergic chronic inflammatory diseases.…”

Section: Chronic Airway Inflammatory Diseasesmentioning

confidence: 95%

“…187 Rh2 inhibited mast cell-induced allergic inflammation via the AKT-Nrf2/NF-κB and p38 MAPK-Nrf2/NF-κB pathways. 187 These studies showed great promise for the use of ginsenosides in the preventive treatment of allergic chronic inflammatory diseases.…”

Section: Chronic Airway Inflammatory Diseasesmentioning

confidence: 95%

Ginsenosides: a potential natural medicine to protect the lungs from lung cancer and inflammatory lung disease

Wang,

Zhang,

Song

et al. 2023

Food Funct.

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Effects of G-Rh2 on mast cell-mediated anaphylaxis via AKT-Nrf2/NF-κB and MAPK-Nrf2/NF-κB pathways

Cited by 25 publications

References 48 publications

Antiallergic Effects of Callerya atropurpurea Extract In Vitro and in an In Vivo Atopic Dermatitis Model

Antiallergic Effects of Callerya atropurpurea Extract In Vitro and in an In Vivo Atopic Dermatitis Model

Activated Mast Cells Combined with NRF2 Predict Prognosis for Esophageal Cancer

Ginsenosides: a potential natural medicine to protect the lungs from lung cancer and inflammatory lung disease

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