Effects of fluorine‐containing usnic acid and fungus <i>Beauveria bassiana</i> on the survival and immune–physiological reactions of Colorado potato beetle larvae

Kryukov, V. Yu.; Tomilova, Oksana G.; Luzina, O. A.; Yaroslavtseva, O. N.; Akhanaev, Yuriy; Tyurin, M. V.; Duisembekov, B. A.; Salakhutdinov, N. F.; Глупов, В. В.

doi:10.1002/ps.4741

Cited by 13 publications

(5 citation statements)

References 55 publications

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“…Moreover, the additional load of cultivable bacteria in the gut promotes the development of fungal infection. Previously, many researchers considered mechanisms of synergy between entomopathogenic fungi and various intestinal or systemic toxicants (including parasitoid venoms) based on different changes in humoral and cellular immune reactions 35,65–70 . However, the impact of the toxicants on microbial communities was insufficiently understood.…”

Section: Discussionmentioning

confidence: 99%

Parasitoid envenomation alters the Galleria mellonella midgut microbiota and immunity, thereby promoting fungal infection

Polenogova

Kabilov

Tyurin

et al. 2019

Sci Rep

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Gut bacteria influence the development of different pathologies caused by bacteria, fungi and parasitoids in insects. Wax moth larvae became more susceptible to fungal infections after envenomation by the ectoparasitoid Habrobracon hebetor . In addition, spontaneous bacterioses occurred more often in envenomated larvae. We analyzed alterations in the midgut microbiota and immunity of the wax moth in response to H. hebetor envenomation and topical fungal infection ( Beauveria bassiana ) alone or in combination using 16S rRNA sequencing, an analysis of cultivable bacteria and a qPCR analysis of immunity- and stress-related genes. Envenomation led to a predominance shift from enterococci to enterobacteria, an increase in CFUs and the upregulation of AMPs in wax moth midguts. Furthermore, mycosis nonsignificantly increased the abundance of enterobacteria and the expression of AMPs in the midgut. Combined treatment led to a significant increase in the abundance of Serratia and a greater upregulation of gloverin. The oral administration of predominant bacteria ( Enterococcus faecalis, Enterobacter sp. and Serratia marcescens ) to wax moth larvae synergistically increased fungal susceptibility. Thus, the activation of midgut immunity might prevent the bacterial decomposition of envenomated larvae, thus permitting the development of fungal infections. Moreover, changes in the midgut bacterial community may promote fungal killing.

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Section: Discussionmentioning

confidence: 99%

Parasitoid envenomation alters the Galleria mellonella midgut microbiota and immunity, thereby promoting fungal infection

Polenogova

Kabilov

Tyurin

et al. 2019

Sci Rep

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“…The (+)-UA enantiomer is prevalent in nature and is commercially available [5]. Chemical modification of UA results in highly active derivatives against mycobacteria [6], viruses [7,8], and insects [9]; practically all the derivatives are less generally toxic than their parent [10,11].…”

Section: Introductionmentioning

confidence: 99%

New Hybrid Compounds Combining Fragments of Usnic Acid and Monoterpenoids for Effective Tyrosyl-DNA Phosphodiesterase 1 Inhibition

et al. 2021

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Usnic acid (UA) is a secondary metabolite of lichens that exhibits a wide range of biological activities. Previously, we found that UA derivatives are effective inhibitors of tyrosyl-DNA phosphodiesterase 1 (TDP1). It can remove covalent complex DNA-topoisomerase 1 (TOP1) stabilized by the TOP1 inhibitor topotecan, neutralizing the effect of the drugs. TDP1 removes damage at the 3′ end of DNA caused by other anticancer agents. Thus, TDP1 is a promising therapeutic target for the development of drug combinations with topotecan, as well as other drugs for cancer treatment. Ten new UA enamino derivatives with variation in the terpene fragment and substituent of the UA backbone were synthesized and tested as TDP1 inhibitors. Four compounds, 11a-d, had IC50 values in the 0.23–0.40 μM range. Molecular modelling showed that 11a-d, with relatively short aliphatic chains, fit to the important binding domains. The intrinsic cytotoxicity of 11a-d was tested on two human cell lines. The compounds had low cytotoxicity with CC50 ≥ 60 μM for both cell lines. 11a and 11c had high inhibition efficacy and low cytotoxicity, and they enhanced topotecan’s cytotoxicity in cancerous HeLa cells but reduced it in the non-cancerous HEK293A cells. This “protective” effect from topotecan on non-cancerous cells requires further investigation.

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“…It is well-known that these toxicants may significantly modulate the susceptibility of hosts to fungal pathogens 10,11 . Various approaches for integrated pest control have been developed on this basis [12][13][14][15][16][17][18] . Researchers usually explain synergy between pathogenic fungi and insecticides by altering the immune response to fungal infections, as well as developmental and behavioral disorders.…”

mentioning

confidence: 99%

“…The rapid development of resistance to various chemical insecticides requires the development of integrated approaches to the control of L. decemlineata populations 38 . One of these approaches may be employing a combination of insecticides and entomopathogens 13,17,39,40 . Previously, we studied the immunophysiological mechanisms of the synergy between the fungus Metarhizium robertsii and the macrocyclic lactones avermectins after a combined treatment to control the Colorado potato beetle 39,40 .…”

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confidence: 99%

Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles

Kryukov

Rotskaya

Yaroslavtseva

et al. 2021

Sci Rep

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Fungal infections and toxicoses caused by insecticides may alter microbial communities and immune responses in the insect gut. We investigated the effects of Metarhizium robertsii fungus and avermectins on the midgut physiology of Colorado potato beetle larvae. We analyzed changes in the bacterial community, immunity- and stress-related gene expression, reactive oxygen species (ROS) production, and detoxification enzyme activity in response to topical infection with the M. robertsii fungus, oral administration of avermectins, and a combination of the two treatments. Avermectin treatment led to a reduction in microbiota diversity and an enhancement in the abundance of enterobacteria, and these changes were followed by the downregulation of Stat and Hsp90, upregulation of transcription factors for the Toll and IMD pathways and activation of detoxification enzymes. Fungal infection also led to a decrease in microbiota diversity, although the changes in community structure were not significant, except for the enhancement of Serratia. Fungal infection decreased the production of ROS but did not affect the gene expression of the immune pathways. In the combined treatment, fungal infection inhibited the activation of detoxification enzymes and prevented the downregulation of the JAK-STAT pathway caused by avermectins. The results of this study suggest that fungal infection modulates physiological responses to avermectins and that fungal infection may increase avermectin toxicosis by blocking detoxification enzymes in the gut.

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Effects of fluorine‐containing usnic acid and fungus Beauveria bassiana on the survival and immune–physiological reactions of Colorado potato beetle larvae

Abstract: Toxicosis caused by FUA provides a stable synergistic effect between FUA and B. bassiana. The combination can be promising for the development of highly efficient products against CPB. © 2017 Society of Chemical Industry.

Cited by 13 publications

References 55 publications

Parasitoid envenomation alters the Galleria mellonella midgut microbiota and immunity, thereby promoting fungal infection

Parasitoid envenomation alters the Galleria mellonella midgut microbiota and immunity, thereby promoting fungal infection

New Hybrid Compounds Combining Fragments of Usnic Acid and Monoterpenoids for Effective Tyrosyl-DNA Phosphodiesterase 1 Inhibition

Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles

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