Effects of extracellular calcium on cell membrane resealing in sonoporation

Zhou, Yun; Shi, Jingyi; Cui, Jianmin; Deng, Cheri X.

doi:10.1016/j.jconrel.2007.11.007

Cited by 107 publications

(101 citation statements)

References 45 publications

(75 reference statements)

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“…At least in principle, this could allow also for separation of cells in which the membrane electrical properties are altered only temporarily, e.g., due to membrane poration by electric field [44], ultrasound [45], or chemical agents (e.g., actinoporins [46] or detergents [5]). The separation efficiency under given experimental conditions could also be improved by optimization of the electric field frequency used for dielectrophoresis under those particular conditions.…”

Section: Discussionmentioning

confidence: 99%

Dielectrophoretic Field-Flow Microchamber for Separation of Biological Cells Based on Their Electrical Properties

Čemažar

Vrtačnik

Amon

et al. 2011

IEEE Trans.on Nanobioscience

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Abstract-We describe the development, fabrication and testing of a microfluidic chamber for dielectrophoretic field-flow separation of biological cells based on their electrical properties. The chamber was constructed from a single Pyrex wafer with interdigitated Au electrodes, a spacer, and a top cover glass, making the events in the chamber observable under most optical microscopes. The dimensions were optimized based on numerical computations of the electric field, its gradient and the fluid-flow velocity profile. The electrodes were fabricated using photolithography. A double-sided self-adhesive tape of 100 m thickness was used as a spacer, with an opening of 80 mm length and 20 mm width cut in its middle to form a channel of 100 m height, and with water-resistant acrylic glue of the tape holding the glass plates together and providing a tight seal. The glue loses its adhesive properties above 70 C, allowing for easy disassembly of the chamber in hot water and its thorough cleaning. A 1:1 mixture of normal and 50 C-heat-treated CHO cells was used to test the chamber. A 93% efficiency of separation was obtained, confirming the usefulness of the chamber in separating cells with sufficient differences in electrical properties of their membranes.

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Section: Discussionmentioning

confidence: 99%

Dielectrophoretic Field-Flow Microchamber for Separation of Biological Cells Based on Their Electrical Properties

Čemažar

Vrtačnik

Amon

et al. 2011

IEEE Trans.on Nanobioscience

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“…The fast resealing of the cell membrane poration was demonstrated by rapid decline of intracellular calcium levels [50,57] and restoration of cell membrane potential [55,[62][63][64].…”

Section: Spatiotemporal Aspects Of Stable Cavitationmentioning

confidence: 99%

Understanding ultrasound induced sonoporation: Definitions and underlying mechanisms

Lentacker

Cock

Deckers

et al. 2014

Advanced Drug Delivery Reviews

646

592

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In the past two decades, research has underlined the potential of ultrasound and microbubbles to enhance drug delivery. However, there is less consensus on the biophysical and biological mechanisms leading to this enhanced delivery. Sonoporation, i.e. the formation of temporary pores in the cell membrane, as well as enhanced endocytosis is reported. Because of the variety of ultrasound settings used -and corresponding microbubble behavior, a clear overview is missing. Therefore, in this review, the mechanisms contributing to sonoporation are categorized according to three ultrasound settings: i) low intensity ultrasound leading to stable cavitation of microbubbles, ii) high intensity ultrasound leading to inertial cavitation with microbubble collapse, and iii) ultrasound application in the absence of microbubbles. Using low intensity ultrasound, the endocytotic uptake of several drugs could be stimulated, while short but intense ultrasound pulses can be applied to induce pore formation and the direct cytoplasmic uptake of drugs. Ultrasound intensities may be adapted to create pore sizes correlating with drug size. Small molecules are able to diffuse passively through small pores created by low intensity ultrasound treatment. However, delivery of larger drugs such as nanoparticles and gene complexes, will require higher ultrasound intensities in order to allow direct cytoplasmic entry.2

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“…We have previously employed a voltage clamp technique to monitor sonoporation (23)(24)(25)(26) in real time by measuring the transmembrane current (TMC) of single Xenopus oocytes. The ultrasound-induced localized membrane disruption allowed ions to flow nonselectively through the membrane, resulting in an increase of the TMC.…”

mentioning

confidence: 99%

Spatiotemporally controlled single cell sonoporation

Fan

Liu

Mayer

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

209

215

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This paper presents unique approaches to enable control and quantification of ultrasound-mediated cell membrane disruption, or sonoporation, at the single-cell level. Ultrasound excitation of microbubbles that were targeted to the plasma membrane of HEK-293 cells generated spatially and temporally controlled membrane disruption with high repeatability. Using whole-cell patch clamp recording combined with fluorescence microscopy, we obtained time-resolved measurements of single-cell sonoporation and quantified the size and resealing rate of pores. We measured the intracellular diffusion coefficient of cytoplasmic RNA/DNA from sonoporation-induced transport of an intercalating fluorescent dye into and within single cells. We achieved spatiotemporally controlled delivery with subcellular precision and calcium signaling in targeted cells by selective excitation of microbubbles. Finally, we utilized sonoporation to deliver calcein, a membrane-impermeant substrate of multidrug resistance protein-1 (MRP1), into HEK-MRP1 cells, which overexpress MRP1, and monitored the calcein efflux by MRP1. This approach made it possible to measure the efflux rate in individual cells and to compare it directly to the efflux rate in parental control cells that do not express MRP1.D espite the development of various approaches for transporting membrane-impermeant compounds (such as fluorescent markers, DNA, RNA, siRNA, proteins, peptides, and amino acids) into living cells (1-3), efficient intracellular delivery of bioactive agents for biomedical applications with minimal adverse effects remains challenging. In addition, it is desirable yet difficult to achieve local perturbation of intracellular processes, which requires subcellular molecular localization inside the living cell (4, 5).Sonoporation uses ultrasound to induce transient disruption of cell membranes (6-8), thereby enabling transport of membraneimpermeant compounds into the cytoplasm of living cells (6,(9)(10)(11). Without the need to use viral vectors, sonoporation enables the delivery of a wide range of bioactive agents with minimal inflammatory and immunological responses for both in vitro studies and in vivo applications (12-16). In addition, ultrasound application can be targeted to a specific volume of tissue in vivo noninvasively. These unique characteristics make sonoporation a compelling and versatile technology for nonviral drug and gene delivery.Sonoporation is typically performed for bulk treatment of a tissue volume in vivo or a large number of cells in vitro, often facilitated by microbubbles that are either injected in the vasculature or mixed in solution with suspended or attached cells. Ultrasound application induces cavitation of the microbubbles (17), signified by rapid volume expansion/contraction and/or collapse (18). These effects generate localized fluid flow, shear stress, and other mechanical or physical impact capable of affecting cells and structures nearby (7,19,20).However, the detailed processes supporting sonoporationmediated transmembrane and ...

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Effects of extracellular calcium on cell membrane resealing in sonoporation

Cited by 107 publications

References 45 publications

Dielectrophoretic Field-Flow Microchamber for Separation of Biological Cells Based on Their Electrical Properties

Dielectrophoretic Field-Flow Microchamber for Separation of Biological Cells Based on Their Electrical Properties

Understanding ultrasound induced sonoporation: Definitions and underlying mechanisms

Spatiotemporally controlled single cell sonoporation

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