Effects of ethynylestradiol and bisphenol A on the development of sea urchin embryos and juveniles

Kiyomoto, Masato; Kikuchi, Asako; Unuma, Tatuya; Yokota, Yukio

doi:10.1007/s00227-005-0208-x

Cited by 22 publications

(22 citation statements)

References 13 publications

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“…The efects of various concentrations of BPA on the development of two sea urchin species Hemicentrotus pulcherrimus and Strongylocentrotus nudus were examined [103]. This study suggested that the sensitivity of sea urchin embryos and juveniles to endocrine disrupter chemicals changes during the stages of development.…”

Section: Efects On Life History Traits and Developmental Eventsmentioning

confidence: 99%

Bisphenol A: Understanding Its Health Effects from the Studies Performed on Model Organisms

Ghosh¹,

Roy²,

Begum³

et al. 2017

Bisphenol a Exposure and Health Risks

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Bisphenol A [4,4′-(propane-2,2-diyl)diphenol] (abbreviated as BPA) is a synthetic xenoestrogenic chemical and endocrine disruptor. It is a most common plasticizer that is used widely to produce epoxy resin and polycarbonate plastics, enters the living system through food and water contamination and generates health hazards. Researches are being conducted to explore the adversity that BPA exerts in living body, and for this reason, model organisms are of scientiic choice. Rodents, zebraish, Drosophila, nematodes, crustaceans and echinoderms are being used for monitoring the efect of BPA on their life history traits, nervous system, endocrine system, reproductive systems, behaviour, etc., which could help us to anticipate what kind of challenges BPA is puting in human life. This systematic review is focused on the latest research trend on BPA toxicity on diferent model organisms.

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Section: Efects On Life History Traits and Developmental Eventsmentioning

confidence: 99%

Bisphenol A: Understanding Its Health Effects from the Studies Performed on Model Organisms

Ghosh¹,

Roy²,

Begum³

et al. 2017

Bisphenol a Exposure and Health Risks

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“…Concentration ranges of test substances were selected according to His et al (1999), King and Riddle (2001) and Fernández and Beiras (2001) for copper, and for TBP, Roepke et al (2005) and Kiyomoto et al (2006) for bisphenol A and Steevens et al (1999) and Pillai et al (2003) for phenanthrene. Eggs and sperm were obtained as described above but eggs were released in general purpose medium 2 (GP 2: 360 mM NaCl, 24.8 mM Na 2 SO 4 , 8.2 mM KCl, 0.74 mM KBr, 0.09 mM Na 2 B 4 O 7 ×10H 2 0, 46.7 mM MgCl 2 ×6H 2 O, 11.9 CaCl 2 ×2H 2 O, 0.08 mM SrCl 2 ×6H 2 O, 2.02 mM NaHCO 3 ) (instead of ASW) which had been aerated for 24 h (U.S. EPA, 1993;Caldwell et al, 2002).…”

Section: In Vitro Fertilization Assaysmentioning

confidence: 99%

Measuring Ca2+-signalling at fertilization in the sea urchin Psammechinus miliaris: Alterations of this Ca2+-signal by copper and 2,4,6-tribromophenol

Schäfer

Bickmeyer

Köehler

2009

Comparative Biochemistry and Physiology Part C: Toxicology &

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During fertilization, eggs undergo a temporary rise in the intracellular concentration of free Ca 2+ ions. Using the membrane permeable acetoxymethylester of the fluorescent calcium indicator dye Fura-2, Fura-2 AM, the Ca 2+ -signal at fertilization was not detectable in eggs of the sea urchin Psammechinus miliaris. However, after treatment of the eggs with Fura-2 AM in combination with MK571, an inhibitor for multidrug resistance associated proteins, clear Ca 2+-signals at fertilization could be measured without microinjection of the dye. We used this methodology to detect possible alterations of Ca 2+ -signalling at fertilization by exposure of eggs to environmental pollutants. For this purpose, the heavy metal copper, the bromophenol 2,4,6-tribromophenol, the organic compound bisphenol A and the polycyclic aromatic hydrocarbon phenanthrene were tested for their potential to inhibit fertilization success of P. miliaris. Copper and 2,4,6-tribromophenol showed a dose-dependent effect on fertilization rates of P. miliaris and significantly inhibited fertilization at 6.3 µM Cu 2+ and 1 µM 2,4,6-tribromphenol. Bisphenol A significantly inhibited fertilization success at 438 µM while phenanthrene had no effect up to 56 µM. 6.3 µM copper and 100 µM 2,4,6-tribromophenol significantly increased the Ca 2+ -signal at fertilization. This alteration may contribute to the reduced fertilization rates of P. miliaris after exposure to copper and 2,4,6-tribromophenol.

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“…Estrone promoted both testicular growth and spermatogenesis (Unuma et al 1999), and increased body weight (Unuma et al 1996). In our previous report (Kiyomoto et al 2006), embryogenesis was affected in a dose-dependent manner at each stage and a typical abnormal morphology was an exogastrula, induced by treatment with β-estradiol or estrone, at a high ratio in Strongylocentrotus nudus and also inducible in Hemicentortus pulcherimus. However, these findings are not sufficient for a complete understanding of the physiological roles of estrogens in echinoderms and the influence of environmental estrogenic compounds on their reproduction and development.…”

Section: Introductionmentioning

confidence: 96%

“…Exogastrulation is induced by estradiol or ethynylestradiol (EER) at relatively high concentrations (3-10 μM) (Mwatibo and Green 1998;Kiyomoto et al 2006), but larval growth and metamorphosis are less sensitive to estrogens and the growth of juveniles is promoted by these chemicals (Kiyomoto et al 2006). Roepke et al (2005) found that sea urchin embryos are sensitive to estrogens and endocrine-disrupting compounds, even at environmentally relevant concentrations, which manifested as a reduction in normal development, and that sensitivity was changed by the administration of antiestrogen chemicals.…”

Section: Introductionmentioning

confidence: 98%

“…The larvae of sea urchins during the feeding stage of metamorphosis appear to be less sensitive to estrogen and develop without morphological abnormalities (Kiyomoto et al 2006), but they may be affected at the molecular level. In vertebrates, the expression of vitellogenin, a precursor of the yolk protein, is often used as a sensitive biomarker of the estrogenic effects of endocrine disruptors (Hiramatsu et al 2006).…”

Section: Introductionmentioning

confidence: 98%

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Exogastrulation and interference with the expression of major yolk protein by estrogens administered to sea urchins

et al. 2008

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Although estrogens have been detected in some echinoderm species, their role is not clearly understood; so we examined the effects of estrogens administered to sea urchin embryos and larvae. A typical malformation was exogastrulation, induced by the exposure to ethynylestradiol (EER) in a defined period of 12 h from 12 h after fertilization (HAF). Morphogenesis for gastrulation was delayed in the treated embryos: protrusion of the archenteron started at 30 HAF when gastrulation had already finished in normal embryos. Exogastrulation induced by EER was cancelled by the antiestrogen chemical, ICI182,780. Feeding larvae were less sensitive to estrogens than those in early embryogenesis and, at certain concentrations, developed without abnormal morphology. The effect of estrogens was examined at the level of gene expression of the major yolk protein (MYP). MYP expression started during the larval stage and was suppressed by estrone at the six-armed stage, but not by beta-estradiol, and in later stage larvae, the expression was not affected by treatment with either estrogen. Estrogens affect sea urchins in the early stage of embryogenesis, leading to abnormal morphogenesis and interference with gene expression.

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Effects of ethynylestradiol and bisphenol A on the development of sea urchin embryos and juveniles

Cited by 22 publications

References 13 publications

Bisphenol A: Understanding Its Health Effects from the Studies Performed on Model Organisms

Bisphenol A: Understanding Its Health Effects from the Studies Performed on Model Organisms

Measuring Ca2+-signalling at fertilization in the sea urchin Psammechinus miliaris: Alterations of this Ca2+-signal by copper and 2,4,6-tribromophenol

Exogastrulation and interference with the expression of major yolk protein by estrogens administered to sea urchins

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