Effects of early cleavage patterns of human embryos on subsequent in vitro development and implantation

Mizobe, Yamato; Oya, Naoto; Iwakiri, Reiko; Yoshida, Naomi; Sato, Yumi; Miyoshi, Katsuhiko; Tokunaga, Makoto; Ezono, Yuji

doi:10.1016/j.fertnstert.2016.04.020

Cited by 33 publications

(23 citation statements)

References 32 publications

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“…Those reports demonstrated that morphokinetic evaluation by time-lapse imaging can provide a more comprehensive and detailed analysis of the developmental events than that of static evaluation and thus may assist in selecting top quality embryos for transfer. Other studies, however, have questioned the benefit of the time-lapse system over the traditional incubators and assessment grading systems [24][25][26][27][28][29].…”

Section: % Of All Biopsy Procedures Performed In Europe Betweenmentioning

confidence: 99%

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Bar-El

Kalma

Malcov

et al. 2016

J Assist Reprod Genet

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Purpose The purpose of the study was to explore the effect of blastomere biopsy for preimplantation genetic diagnosis (PGD) on the embryos' dynamics, further cleavage, development, and implantation. Methods The study group included 366 embryos from all PGD treatments (September 2012 to June 2014) cultured in the EmbryoScope™ time-lapse monitoring system. The control group included all intracytoplasmic sperm injection (ICSI) embryos cultured in EmbryoScope™ until day 5 during the same time period (385 embryos). Time points of key embryonic events were analyzed with an EmbryoViewer™. Results Most (88 %) of the embryos were biopsied at ≥8 cells. These results summarize the further dynamic development of the largest cohort of biopsied embryos and demonstrate that blastomere biopsy of cleavage-stage embryos significantly delayed compaction and blastulation compared to the control non-biopsied embryos. This delay in preimplanation developmental events also affected postimplantation development as observed when the dynamics of non-implanted embryos (known implantation data (KID) negative) were compared to those of implanted embryos (KID positive). Conclusion Analysis of morphokinetic parameters enabled us to explore how blastomere biopsy interferes with the dynamic sequence of developmental events. Our results show that biopsy delays the compaction and the blastulation of the embryos, leading to a decrease in implantation.

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Section: % Of All Biopsy Procedures Performed In Europe Betweenmentioning

confidence: 99%

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Bar-El

Kalma

Malcov

et al. 2016

J Assist Reprod Genet

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“…Time-lapse (TL) imaging of developing embryos offers more accurate quantification of cellular kinetics and cell cycle events than was possible with conventional static morphology assessment. A wide array of kinetic markers have been proposed to be predictive for continued embryonic development and blastocyst formation (8,(10)(11)(12)(13)(14)(15)(16). TL has also revealed the prevalence of specific dysmorphisms such as multinucleation (MU), reverse cleavage (RC), direct uneven cleavage (DUC), and irregular chaotic division (ICD) in human preimplantation embryos (10,(17)(18)(19)(20).…”

mentioning

confidence: 99%

Are cleavage anomalies, multinucleation, or specific cell cycle kinetics observed with time-lapse imaging predictive of embryo developmental capacity or ploidy?

Desai

Goldberg

Austin

et al. 2018

Fertility and Sterility

110

103

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“…When embryos show high synchrony of the first division and sufficient numbers are likely to develop into blastocysts, all the blastocysts should be cryopreserved, instead of early embryo transfer. Cryopreserved embryo transfer, which provides higher pregnancy rates, can be performed after the next cycle. In the present study, cryopreserved‐thawed blastocyst transfer achieved good pregnancy rates for all groups, suggesting that this was an effective transfer technique.…”

Section: Discussionmentioning

confidence: 99%

“…The smaller the time lag, the greater the synchrony. The study used 25.90 hours as a reference value for the definition of early‐cleavage embryos in the first division, according to the authors’ previous study . The average time of synchrony of the first division in a single oocyte retrieval cycle was 3.96 hours for 137 cycles.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have demonstrated the methods for selecting good embryos, with a focus on the division speed and morphology of early‐stage embryos . Although the early‐stage transfer of embryos that are selected by using a time‐lapse system does not increase pregnancy rates, blastocyst transfer does so . Thus, the use of a time‐lapse system is important in order to determine whether embryos have reached the blastocyst stage at an early stage of culture.…”

Section: Introductionmentioning

confidence: 99%

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Synchrony of the first division as an index of the blastocyst formation rate during embryonic development

Mizobe¹,

Tokunaga²,

Oya³

et al. 2017

Reprod Medicine & Biology

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PurposeTo devise an uninvasive selection system for human embryos with high developmental potential after a single oocyte retrieval cycle by comparing the in vitro and in vivo effectiveness of first division synchrony against subsequent embryonic developmental stages.MethodsThe effects of using assisted reproductive technology on 948 embryos that were produced in 137 cycles were examined by dividing the embryos into “early cleavage” (first division within 25.90 hours) and “late cleavage” (first division at or after 25.90 hours) groups and comparing the blastocysts and good‐quality blastocyst formation rates between the two groups. These two groups were each divided further into “high synchrony” (first division synchrony within 3.96 hours) and “low synchrony” (first division synchrony at or after 3.96 hours) groups. The blastocysts, good‐quality blastocyst formation rates, and pregnancy rates were compared among these four groups.ResultsBoth the blastocysts and good‐quality blastocyst formation rates were significantly higher in the early‐cleavage groups than in the late‐cleavage groups. The blastocyst formation rate of the latter was also significantly increased in the high‐synchrony, compared with the low‐synchrony, group.ConclusionFirst division synchrony in a single oocyte retrieval cycle could be a useful assessment of the blastocyst formation rate that enables the selection of viable embryos at an early stage of culture.

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Effects of early cleavage patterns of human embryos on subsequent in vitro development and implantation

Abstract: Transfer of blastocysts derived from embryos that completed first and second divisions within 25.90 and 37.88 hours after culture, respectively, brings about high pregnancy rates.

Cited by 33 publications

References 32 publications

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Are cleavage anomalies, multinucleation, or specific cell cycle kinetics observed with time-lapse imaging predictive of embryo developmental capacity or ploidy?

Synchrony of the first division as an index of the blastocyst formation rate during embryonic development

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