Effects of Different Suture Materials Used for the Repair of Hypospadias: A Stereological Study in a Rat Model

Abstract

Introduction: Repair of the penile urethra in hypospadias is done with different suture materials. The aim of this study was to quantify the impact of different suture threads on histological parameters of the penis and urethra following hypospadias repair in rats. Materials and Methods: Six groups of the rats, except for the control group, underwent incision and repair of the penis and urethra in the ventral line using different suture materials including Chromic, Vicryl (polyglactic acid), PDS (polydioxanone), Rapid Vicryl (polyglactin 910), Monocryl (poliglecaprone 25) and control groups. After 3 weeks the body of the penis was excised and histological sections were studied using stereological methods. Results: Monocryl (poliglecaprone 25) was associated with a higher percentage of vessel density, higher volume of urethral lumen and lower lymphocyte infiltration. Volume of urethral epithelium was highest in the PDS group compared to the other operated groups. There was no significant difference regarding collagen deposition and fibroblast infiltration among the operated groups. Conclusions: The Monocryl and PDS groups showed better results.

Grade of hypospadias is the only factor predicting for re-intervention after primary hypospadias repair: A multivariate analysis from a cohort of 474 patients

“…The ideal suture material should be waterproof, last long enough to allow sufficient healing, but be resorbed in a time sufficient to avoid excessive scar formation and fibrous tissue. Small series retrospectively comparing two suture types [21,22], or evaluating suture material in animals [23] led us to hypothesize that monofilament absorbable suture could be superior to polyfilament. The rationale was that a monofilament suture attracts less, or no, fluid and urine into the tissues compared with multifilament suture, because of capillarity.…”

Quantitative Evaluation of Inflammatory Response in Rats Submitted to the Abdominal Wall Surgery Using Peritoneum of Paca Preserved in 300% Supersaturated Solution of Sucrose or 98% Glycerin

“…The microtome of this material was carried out in an automatic microtome (Leica RM 2155), using glass knives, to obtain 6 sections (3 pairs) with 2 µm per block at 12 µm intervals previously determined through a pilot experiment, which measured the size of the cell under evaluation-section sampling fraction (ssf 4/30,000). After performing this procedure, the sampled sections were collected on slides and subject to HE staining, according to [21].…”

“…Stereology presents advantages over qualitative studies, such as histology, since the results are quantitative instead of qualitative, being, therefore, replicable in any other laboratory, in addition to the well-defined statistical groundwork which supports this method. For evaluating the inflammatory response, stereology was used by [21] in a comparative study of the healing process in different suture wires in the repair of hypospadia in rats.…”

Does the Choice of Suture Material Affect the Incidence of Urethrocutaneous Fistulae After Hypospadias Repair? – A Comparison of Polyglactin 910 with Polydioxanone

“…We aimed to address the last question in this study and focused on the two most commonly used suture materials used worldwide, polyglactin 910 and polydioxanone. There are numerous studies about suture material and how they behave in living tissue or on exposure to human urine 9,10 . Some have compared different sutures used with different techniques 11 .…”