Effects of Different Prenatal Nutrition Strategies on the Liver Metabolome of Bulls and Its Correlation with Body and Liver Weight

Polizel, Guilherme Henrique Gebim; Cançado, Fernando Augusto Correia Queiroz; Fernandes, Arícia Christofaro; Cracco, Roberta Cavalcante; Carmona, Brenda Tonsic; Castellar, Henrique Hespanhol; Poleti, Mirele Daiana; Santana, Miguel Henrique de Almeida

doi:10.3390/metabo12050441

Cited by 6 publications

(8 citation statements)

References 56 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…During the rearing period, the young bulls received two types of supplements: an energetic supplement in the dry season (winter); and a protein supplement in the wet season (summer). Details of both supplements can be found in Polizel et al [ 8 ]. From calving to the start of the finishing phase, the young bulls grazed on Brachiaria brizantha cv.…”

Section: Methodsmentioning

confidence: 99%

“…More details about the phenotypic effects on the bulls, and the finishing and rearing phase can be found in Polizel et al [ 8 , 9 , 10 ].…”

Section: Methodsmentioning

confidence: 99%

“…The main economic interest traits in beef cattle production (growth and muscle development) are dependent on genetics and post-natal environmental conditions [ 1 , 2 ]. However, prenatal life can also impact these characteristics and others as immunology, stress response, reproduction, microbiota, organ development, and metabolic changes [ 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. These alterations may persist long-term in an offspring’s life—a concept known as fetal programming.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impacts of Different Prenatal Supplementation Strategies on the Plasma Metabolome of Bulls in the Rearing and Finishing Phase

Polizel¹,

Fernandes²,

Furlan³

et al. 2023

Metabolites

Self Cite

View full text Add to dashboard Cite

This study investigated the effects of maternal nutrition on the plasma metabolome of Nellore bulls in the rearing and finishing phases, and metabolic differences between these phases. For this study, three nutritional approaches were used in 126 cows during pregnancy: NP—(control) mineral supplementation; PP—protein-energy supplementation in the final third; and FP—protein-energy supplementation during the entire pregnancy. We collected blood samples from male offspring in the rearing (450 ± 28 days old) and finishing phases (660 ± 28 days old). The blood was processed, and from plasma samples, we performed the targeted metabolome analysis (AbsoluteIDQ® p180 Kit). Multiple linear regression, principal component analysis (PCA), repeated measures analysis over time, and an enrichment analysis were performed. PCA showed an overlap of treatments and time clusters in the analyses. We identified significant metabolites among the treatments (rearing phase = six metabolites; finishing phase = three metabolites) and over time (21 metabolites). No significant metabolic pathways were found in the finishing phase, however, we found significant pathways in the rearing phase (Arginine biosynthesis and Histidine metabolism). Thus, prenatal nutrition impacted on plasma metabolome of bulls during the rearing and finishing phase and the different production stages showed an effect on the metabolic levels of bulls.

show abstract

Section: Methodsmentioning

confidence: 99%

“…More details about the phenotypic effects on the bulls, and the finishing and rearing phase can be found in Polizel et al [ 8 , 9 , 10 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impacts of Different Prenatal Supplementation Strategies on the Plasma Metabolome of Bulls in the Rearing and Finishing Phase

Polizel¹,

Fernandes²,

Furlan³

et al. 2023

Metabolites

Self Cite

View full text Add to dashboard Cite

show abstract

“…Marandu). In the rearing period, the animals received an energy supplement of 0.3% of the average weight of the batch during the 6 months in the dry season (winter), (TDN = 67.55%; CP = 24.78%; NDF = 11.24%; fat = 2.61%; 0.3% of BW), and protein supplementation in the 6 months following, which corresponds with the wet season (summer), (TDN = 53.15%; CP = 30.03%; NDF = 9.14%; fat = 1.65%; 0.1% of BW) with the offer of 0.1% of the average batch weight, until the animals entered the feedlot, according to Polizel et al [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Fetal Programming and Its Effects on Meat Quality of Nellore Bulls

Christofaro Fernandes,

Beline,

Polizel

et al. 2023

Veterinary Sciences

Self Cite

View full text Add to dashboard Cite

This work aimed to evaluate the effects of prenatal nutritional stimulation at different pregnancy stages on carcass traits and meat quality in bovine progeny. For this purpose, 63 Nellore bulls, born from cows submitted to three nutritional plans, were used: not programmed (NP), which did not receive protein supplementation; partially programmed (PP), which had protein-energy supplementation (0.3% of mean body weight of each batch) only in the final third of pregnancy; and full programming (FP), which received supplementation (0.3% of mean body weight of each batch) throughout pregnancy. The averages of parameters were submitted to the ANOVA, and the supplementation periods, which were different when p value < 0.05, were compared. Carcass weights and rib eye area (REA) did not differ between treatments (p > 0.05), but subcutaneous fat thickness (SFT) showed a tendency (p = 0.08) between groups. For lipids and marbling, no differences were found (p > 0.05). In the analyses of maturation time and shelf life, no difference was observed between treatments. However, there was a tendency between treatments at 14 days of maturation time for cooking loss (CL) (p = 0.08). Treatments did not affect shear force in the progenies (p > 0.05). Fetal programming had no effect on the meat quality of Nellore bulls.

show abstract

“…Some studies have already tested different feeding strategies and characterized the metabolome of animals destined for meat production [ 12 , 21 , 22 , 23 , 24 , 25 ]. However, few studies have investigated the consequences of prenatal nutrition on the metabolism of their offspring [ 26 , 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolomics Changes in Meat and Subcutaneous Fat of Male Cattle Submitted to Fetal Programming

Fernandes,

Polizel,

Cracco

et al. 2023

Metabolites

Self Cite

View full text Add to dashboard Cite

This study investigated changes in meat and subcutaneous fat metabolomes and possible metabolic pathways related to prenatal nutrition in beef cattle. For this purpose, 18 Nellore bulls were used for meat sampling and 15 for fat sampling. The nutritional treatments during the gestation were: NP—not programmed or control, without protein-energy supplementation; PP—partially programmed, with protein-energy supplementation (0.3% of body weight (BW)) only in the final third of pregnancy; and FP—full programming, with protein-energy supplementation (0.3% of BW) during the entire pregnancy. The meat and fat samples were collected individually 24 h after slaughter, and the metabolites were extracted using a combination of chemical reagents and mechanical processes and subsequently quantified using liquid chromatography or flow injection coupled to mass spectrometry. The data obtained were submitted to principal component analysis (PCA), analysis of variance (ANOVA), and functional enrichment analysis, with a significance level of 5%. The PCA showed an overlap between the treatments for both meat and fat. In meat, 25 metabolites were statistically different between treatments (p ≤ 0.05), belonging to four classes (glycerophospholipids, amino acids, sphingolipids, and biogenic amine). In fat, 10 significant metabolites (p ≤ 0.05) were obtained in two classes (phosphatidylcholine and lysophosphatidylcholine). The functional enrichment analysis showed alterations in the aminoacyl-tRNA pathway in meat (p = 0.030); however, there was no pathway enriched for fat. Fetal programming influenced the meat and fat metabolomes and the aminoacyl-tRNA metabolic pathway, which is an important candidate for the biological process linked to meat quality and related to fetal programming in beef cattle.

show abstract

Effects of Different Prenatal Nutrition Strategies on the Liver Metabolome of Bulls and Its Correlation with Body and Liver Weight

Cited by 6 publications

References 56 publications

Impacts of Different Prenatal Supplementation Strategies on the Plasma Metabolome of Bulls in the Rearing and Finishing Phase

Impacts of Different Prenatal Supplementation Strategies on the Plasma Metabolome of Bulls in the Rearing and Finishing Phase

Fetal Programming and Its Effects on Meat Quality of Nellore Bulls

Metabolomics Changes in Meat and Subcutaneous Fat of Male Cattle Submitted to Fetal Programming

Contact Info

Product

Resources

About