Effects of different intensities of extremely low frequency pulsed electromagnetic fields on formation of osteoclast‐like cells

Chang, Kuo-Ming; Chang, Walter Hong‐Shong; Wu, Ming-Hsien; Shih, Chun Kuang

doi:10.1002/bem.10118

Cited by 64 publications

(45 citation statements)

References 38 publications

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“…No study has evaluated the action of PEMF irradiation on the production of cytokines in fibroblast-like cell cultures, and the most similar data refer to the post-irradiation release of cytokines in osteoblasts [20] and in the osteoclast-like cells [8]. In both studies, ELISA was used for quantification and demonstrated an increase of TNF-a, IL-1b and PG-E 2 in relation both to the recruitment of the osteoclast-like cells and to the intensity of the electrical field.…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Pulsed electromagnetic fields decrease proinflammatory cytokine secretion (IL-1β and TNF-α) on human fibroblast-like cell culture

Gómez-Ochoa

Gómez-Casal

et al. 2010

Rheumatol Int

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The clinical use of pulsed electromagnetic fields (PEMF) in osteoarticular pathology is widely extended, although the mechanisms involved are unknown. The aim of this study was to evaluate the action of a new protocol of treatment with PEMF on liquid medium cultures of fibroblast-like cells derivates of mononuclear peripheral blood cells. Fibroblast-like cells growth was obtained in liquid medium culture from mononuclear cells (MNC) of human peripheral blood. The PEMF irradiation protocol included an intensity of 2.25 mT, a frequency of 50 Hz and an application time of 15 min on days 7, 8 and 9 of cell culture. Immunophenotype was performed with specific heterologous monoclonal antibodies for each cell receptor (Vimentin, Cytokeratin, CD34, CD41, CD61 and CD68). The cytokines' production was determined in the supernatant of the culture medium by means of the Luminex technology. The immunophenotype did not show any statistical difference on comparing treated against non-treated cell cultures on any of the days. In the treatment cell population, the proinflammatory cytokines, IL-1β and TNF-α showed a significant decrease on days 14 and 21 of the culture, whilst IL-10 increased significantly on day 21. It is concluded that PEMF irradiation does not alter the cell immunophenotype of the fibroblast-like cell population, but does provoke a decrease in the production of inflammatory-type cytokines (IL-1β, TNF-α) and an increase in cytokines of lymphocytic origin (IL-10). These facts coincide with the chronology of the clinical effect undergone by patients with osteoarticular pathology after PEMF irradiation.

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Section: Discussionmentioning

confidence: 97%

“…Since then, studies have been made of the action on osteoblast cultures [11], osteoclasts [8], chondrocytes [18] and, recently, on human bone marrow mesenchymal stem cells (MSC) [33]. It is remarkable that this selection, based on its close relationship with the muscle-skeletal system, does not include fibroblast-like cells [6].…”

Section: Introductionmentioning

confidence: 98%

Pulsed electromagnetic fields decrease proinflammatory cytokine secretion (IL-1β and TNF-α) on human fibroblast-like cell culture

Gómez-Ochoa

Gómez-Casal

et al. 2010

Rheumatol Int

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“…The levels of expression of SP and TNF-a are known to be affected by exposure to ionizing radiation, for example, radiotherapy [Forsgren et al, 2000]. Indications of an effect of non-ionizing radiation on the release of TNF-a and other cytokines have been reported [Chang et al, 2003], but results from other studies contradict this [Ikeda et al, 2003]. Non-ionizing radiation has also been shown to affect the concentration and activity of BDNF after sciatic nerve transection in the rat [Longo et al, 1999].…”

Section: Introductionmentioning

confidence: 97%

No effect of mobile phone‐like RF exposure on patients with atopic dermatitis

Johansson

Forsgren

Stenberg

et al. 2008

Bioelectromagnetics

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This study investigates the effect of exposure to a mobile phone-like radiofrequency (RF) electromagnetic field on people with atopic dermatitis (AD). Fifteen subjects with AD were recruited and matched with 15 controls without AD. The subjects were exposed for 30 min to an RF field at 1 W/kg via an indoor base station antenna attached to a 900 MHz GSM mobile phone. Blood samples for ELISA analysis of the concentration of substance P (SP), tumor necrosis factor receptor 1 (TNF R1), and brain derived neurotrophic factor (BDNF) in serum were drawn before and after the provocation (exposure/sham). Baseline heart rate and heart rate variability, local blood flow, and electrodermal activity were also recorded. No significant differences between the subject groups were found for baseline neurophysiological data. The cases displayed a serum concentration of TNF R1 significantly higher than the control subjects and a significantly lower serum concentration of BDNF in the baseline condition. For SP there was no difference between groups. However, no effects related to RF exposure condition were encountered for any of the measured substances. As to symptoms, a possible correlation with exposure could not be evaluated, due to too few symptom reports. The result of the study does not support the hypothesis of an effect of mobile phone-like RF exposure on serum levels of SP, TNF R1, and BDNF in persons with AD.

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“…Several studies have shown that applied EFs stimulate cell proliferation and differentiation, as well as synthesis of growth factors and matrix proteins [24][25][26][27]. The potential of electrical stimulation for soft tissues also has been explored in cartilage and ligaments.…”

Section: Introductionmentioning

confidence: 99%

Effects of Applied DC Electric Field on Ligament Fibroblast Migration and Wound Healing

Chao

Hung

et al. 2007

Connective Tissue Research

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Applied electric fields (static and pulsing) are widely used in orthopedic practices to treat nonunions and spine fusions and have been shown to improve ligament healing in vivo. Few studies, however, have addressed the effect of electric fields (EFs) on ligament fibroblast migration and biosynthesis. In the current study, we applied static and pulsing direct current (DC) EFs to calf anterior cruciate ligament (ACL) fibroblasts. ACL fibroblasts demonstrated enhanced migration speed and perpendicular alignment to the applied EFs. The motility of ligament fibroblasts was further modulated on type I collagen. In addition, type I collagen expression increased in ACL fibroblasts after exposure to pulsing EFs. In vitro wound-healing studies showed inhibitory effects of static EFs, which were alleviated with a pulsing EF. Our results demonstrate that applied EFs augment ACL fibroblast migration and biosynthesis and provide potential mechanisms by which EFs may be used for enhancing ligament healing and repair.

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Effects of different intensities of extremely low frequency pulsed electromagnetic fields on formation of osteoclast‐like cells

Cited by 64 publications

References 38 publications

Pulsed electromagnetic fields decrease proinflammatory cytokine secretion (IL-1β and TNF-α) on human fibroblast-like cell culture

Pulsed electromagnetic fields decrease proinflammatory cytokine secretion (IL-1β and TNF-α) on human fibroblast-like cell culture

No effect of mobile phone‐like RF exposure on patients with atopic dermatitis

Effects of Applied DC Electric Field on Ligament Fibroblast Migration and Wound Healing

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