Effects of diammonium glycyrrhizinate on random skin flap survival in rats: An experimental study

Lv, Qingyu; Gao, Xiang; Lin, Dingsheng; Chen, Yun; Cao, Bin; Zhou, Kailiang

doi:10.3892/br.2016.733

Cited by 8 publications

(5 citation statements)

References 36 publications

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“…[6][7][8][9][10][11][12] The evaluation of the results of interventions performed in experimental models of skin grafts and flaps include some methods, such as gene expression analysis, 10,13 protein expression analysis, 11,12,[14][15][16] assessment of histological changes, 11,12,15 and the percentage of viable tissue. 6,7,17,18 The latter is widely used for being fast, inexpensive, and efficient. Three methods can be used to evaluate the percentage of viable tissue: weight of paper template (WPT), 17,18 paper template area (PTA), 19 and photographic area (PA).…”

Section: Introductionmentioning

confidence: 99%

“…6,7,17,18 The latter is widely used for being fast, inexpensive, and efficient. Three methods can be used to evaluate the percentage of viable tissue: weight of paper template (WPT), 17,18 paper template area (PTA), 19 and photographic area (PA). 11,14,16 The paper template method was first described by Sasaki and Pang in 1980 20 and since then it has been used to evaluate the percentage of flap necrosis on the seventh postoperative day.…”

Section: Introductionmentioning

confidence: 99%

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A Comparison of Three Methods for the Analysis of Skin Flap Viability: Reliability and Validity

Tim

Martignago

Silva

et al. 2018

Advances in Wound Care

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Technological advances have provided new alternatives to the analysis of skin flap viability in animal models; however, the interrater validity and reliability of these techniques have yet to be analyzed. The present study aimed to evaluate the interrater validity and reliability of three different methods: weight of paper template (WPT), paper template area (PTA), and photographic analysis. Sixteen male Wistar rats had their cranially based dorsal skin flap elevated. On the seventh postoperative day, the viable tissue area and the necrotic area of the skin flap were recorded using the paper template method and photo image. The evaluation of the percentage of viable tissue was performed using three methods, simultaneously and independently by two raters. The analysis of interrater reliability and viability was performed using the intraclass correlation coefficient and Bland Altman Plot Analysis was used to visualize the presence or absence of systematic bias in the evaluations of data validity. The results showed that interrater reliability for WPT, measurement of PTA, and photographic analysis were 0.995, 0.990, and 0.982, respectively. For data validity, a correlation >0.90 was observed for all comparisons made between the three methods. In addition, Bland Altman Plot Analysis showed agreement between the comparisons of the methods and the presence of systematic bias was not observed. Digital methods are an excellent choice for assessing skin flap viability; moreover, they make data use and storage easier. Independently from the method used, the interrater reliability and validity proved to be excellent for the analysis of skin flaps' viability.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Comparison of Three Methods for the Analysis of Skin Flap Viability: Reliability and Validity

Tim

Martignago

Silva

et al. 2018

Advances in Wound Care

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“…Besides the use of iron chelation, superoxide dismutase (SOD) has been recognized as a hopeful strategy to enhance the outcome of TBI ( Kobeissy et al, 2016 ). The function of SOD is to prevent the production of OH, thereby reducing brain damage ( Lv et al, 2016 ). To decrease the occurrence of ferroptosis, cells make use of antioxidants like GSH and NAC to remove an excess of oxidative free radicals.…”

Section: Ferroptosis and Mitochondrial Dysfunction In Tbimentioning

confidence: 99%

Ferroptosis and mitochondrial dysfunction in acute central nervous system injury

Dong

Gong

Zhao

et al. 2023

Front. Cell. Neurosci.

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Acute central nervous system injuries (ACNSI), encompassing traumatic brain injury (TBI), non-traumatic brain injury like stroke and encephalomeningitis, as well as spinal cord injuries, are linked to significant rates of disability and mortality globally. Nevertheless, effective and feasible treatment plans are still to be formulated. There are primary and secondary injuries occurred after ACNSI. Most ACNSIs exhibit comparable secondary injuries, which offer numerous potential therapeutic targets for enhancing clinical outcomes. Ferroptosis, a newly discovered form of cell death, is characterized as a lipid peroxidation process that is dependent on iron and oxidative conditions, which is also indispensable to mitochondria. Ferroptosis play a vital role in many neuropathological pathways, and ACNSIs may induce mitochondrial dysfunction, thereby indicating the essentiality of the mitochondrial connection to ferroptosis in ACNSIs. Nevertheless, there remains a lack of clarity regarding the involvement of mitochondria in the occurrence of ferroptosis as a secondary injuries of ACNSIs. In recent studies, anti-ferroptosis agents such as the ferroptosis inhibitor Ferrostain-1 and iron chelation therapy have shown potential in ameliorating the deleterious effects of ferroptosis in cases of traumatic ACNSI. The importance of this evidence is extremely significant in relation to the research and control of ACNSIs. Therefore, our review aims to provide researchers focusing on enhancing the therapeutic outcomes of ACNSIs with valuable insights by summarizing the physiopathological mechanisms of ACNSIs and exploring the correlation between ferroptosis, mitochondrial dysfunction, and ACNSIs.

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“…Previous studies have shown attempts to enhance the tolerance of the skin flaps to ischemia and congestion using different drug preparations 1 , 4 . Various agents have been stated to be beneficial in improving flap survival through inhibiting inflammation, getting rid of free radicals, improving angiogenesis, and/or increasing the microvascular network 1 .…”

Section: Introductionmentioning

confidence: 99%

Experimental study of the effects of nitroglycerin, botulinum toxin A, and clopidogrel on bipedicled superficial inferior epigastric artery flap survival

Ellabban

Elmasry

Abdelrahman

et al. 2022

Sci Rep

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Beneficial effects could be achieved by various agents such as nitroglycerin, botulinum toxin A (BoTA), and clopidogrel to improve skin flap ischaemia and venous congestion injuries. Eighty rats were subjected to either arterial ischaemia or venous congestion and applied to a bipedicled U-shaped superficial inferior epigastric artery (SIEA) flap with the administration of nitroglycerin, BoTA, or clopidogrel treatments. After 7 days, all rats were sacrificed for flap evaluation. Necrotic area percentage was significantly minimized in flaps treated with clopidogrel (24.49%) versus the ischemic flaps (34.78%); while nitroglycerin (19.22%) versus flaps with venous congestion (43.26%). With ischemia, light and electron microscopic assessments revealed that nitroglycerin produced degeneration of keratinocytes and disorganization of collagen fibers. At the same time, with clopidogrel administration, there was an improvement in the integrity of these structures. With venous congestion, nitroglycerin and BoTA treatments mitigated the epidermal and dermal injury; and clopidogrel caused coagulative necrosis. There was a significant increase in tissue gene expression and serum levels of vascular endothelial growth factor (VEGF) in ischemic flaps with BoTA and clopidogrel, nitroglycerin, and BoTA clopidogrel in flaps with venous congestion. With the 3 treatment agents, gene expression levels of tumor necrosis factor-α (TNF-α) were up-regulated in the flaps with ischemia and venous congestion. With all treatment modalities, its serum levels were significantly increased in flaps with venous congestion and significantly decreased in ischemic flaps. Our analyses suggest that the best treatment option for ischemic flaps is clopidogrel, while for flaps with venous congestion are nitroglycerin and BoTA.

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Effects of diammonium glycyrrhizinate on random skin flap survival in rats: An experimental study

Cited by 8 publications

References 36 publications

A Comparison of Three Methods for the Analysis of Skin Flap Viability: Reliability and Validity

A Comparison of Three Methods for the Analysis of Skin Flap Viability: Reliability and Validity

Ferroptosis and mitochondrial dysfunction in acute central nervous system injury

Experimental study of the effects of nitroglycerin, botulinum toxin A, and clopidogrel on bipedicled superficial inferior epigastric artery flap survival

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