Effects of Cultivation Media on In vitro Callus Induction and Regeneration Capabilities of Pakaumpuel Rice (Oryza sativa L.), Thai Rice Landrace

Trunjaruen, Attachai; Raso, Sayam; Maneerattanarungroj, Pitakpong; Taratima, Worasitikulya

doi:10.48048/wjst.2020.5349

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…is result also concurred with many previous reports that BA and NAA can be used for plantlet regeneration from rice callus [43,45,46]. However, shoot formation in our experiment was more dominant than root formation for high BA : NAA ratios.…”

Section: Resultssupporting

confidence: 93%

“…MS medium supplemented with all concentrations of 2,4-D promoted calli formation in Luem Pua rice seed. Previous studies also concurred that appropriate 2,4-D concentration promoted callus formation by encouraging embryogenic capability on the scutellar cells, resulting in proliferation and expansion of rice embryogenic calli [41][42][43][44]. New plantlets from calli of Luem Pua rice were regenerated after culture on MS medium with BA and NAA.…”

Section: Resultsmentioning

confidence: 68%

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Salinity Stress Response of Rice (Oryza sativa L. cv. Luem Pua) Calli and Seedlings

2022

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Soil salinity limits plant growth and production. This research investigated a suitable medium for callus induction and plantlet regeneration in the Luem Pua rice cultivar. The effect of salt stress on seedling growth was determined using in vitro culture and soil conditions. An efficient protocol for callus induction has been developed by culture sterilized seeds on the Murashige and Skoog (MS, 1962) medium containing 0.5 mg/l benzyladenine (BA) with 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) that resulted in a 100% callus induction. Plantlet regeneration percentage of 49% was recorded on the MS medium containing 4 mg/l BA with 0.5 mg/l 1-naphthaleneacetic acid (NAA) after 4 weeks. For salt stress investigation, the calli were treated on an induction medium containing various concentrations of NaCl (0, 50, 100, 150, and 200 mM), while two-week-old rice seedlings were planted in soil and treated with the same concentration of NaCl for 4 weeks. In vitro culture revealed that callus survival percentage decreased when NaCl concentration increased, similar to soil culture. Seedling growth under salinity treatment also decreased when NaCl concentration increased, while other physiological parameters such as total chlorophyll, chlorophyll a, chlorophyll b, green intensity, and chlorophyll fluorescence under light conditions increased under salinity stress. These changes define the growth and physiological salinity tolerance characteristics of Luem Pua rice calli and seedlings. They can be utilized as a baseline for demand-driven in vitro rice propagation, providing useful information that can be combined with other agronomic features in rice development or breeding programs to improve the flexibility of abiotic stress-tolerant cultivars.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 68%

Salinity Stress Response of Rice (Oryza sativa L. cv. Luem Pua) Calli and Seedlings

2022

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“…Callus induction from bamboo ( Dendrocalamus hamiltonii ) was carried out by shoot tip culture on medium with 3 mg/l 2,4-D and 1 mg/l benzyladenine ( Zang et al, 2016 ). Callus induction medium with 2 mg/l 2,4-D was identified as the optimal condition for landrace rice ( Oryza sativa ) from Thailand ( Trunjaruen et al, 2020 ). Auxins, e.g., 2,4-D and NAA in callus induction medium were reported to promote expression of important genes like LBD family and ARF19 ( Ikeuchi, Sugimoto & Iwase, 2013 ), resulting in callus formation.…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of pokeweed (Phytolacca americana L.) and comparison of phenolic, flavonoid content, and antioxidant activity between pokeweed callus and other parts

Trunjaruen

Luecha

Taratima

2022

PeerJ

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Background Pokeweed (Phytolacca americana L.) is regarded as an invasive plant in many parts of the world but possesses therapeutic characteristics used for antitumor and rheumatism treatment. This study investigated the effects of auxins and four explants on pokeweed callus induction. The effects of cytokinins and combinations between cytokinins and NAA on shoot and root induction were also studied. TPC, TFC and antioxidant activity of calli were screened and compared with other pokeweed plant parts. Methods Four explants were used to induce callus using 2,4-D and IBA at 1, 2, 3 and 4 mg/l for each auxin. Direct shoot organogenesis from nodal explants was investigated using BAP, kinetin and TDZ (1, 2 and 4 mg/l for each cytokinin). Combined effects between cytokinins and NAA at 0.1, 0.2 and 0.3 mg/l were further simultaneously estimated with root induction. Calli derived from the leaves were compared with other plant parts for TPC, TFC and antioxidant activity using the Folin-Ciocalteu, AlCl3 colorimetric assay and DPPH assays, respectively. Results Results showed that MS medium containing 2 mg/l 2,4-D induced callus formation on leaf explants that provided highest fresh and dry weights. Three types of synthetic cytokinins as kinetin, TDZ and BAP were used for direct shoot organogenesis from pokeweed nodes. MS medium containing 2 mg/l kinetin was effective in stimulating normal shoots, with the largest number of shoots and leaves and the longest shoots. The combination between cytokinins and NAA showed no positive effect on shoot and root induction from pokeweed nodal explants. For TPC and TFC determination, pokeweed seeds and leaves possessed the highest phenolic and flavonoid contents, respectively. Highest phenolic content of pokeweed seeds led to lowest IC50 by DPPH assay. Phenolic content was higher than flavonoid content. Conclusion Results suggested promising conditions for callus induction. Leaf explants cultured on MS medium with 2 mg/l 2,4-D and nodal explants cultured on MS medium with 2 mg/l kinetin provided the largest number of normal shoots and leaves. NAA did not show positive effects on shoot and root induction when combined with cytokinins. Chemical constituent screening indicated that seeds and leaves provided highest TPC and TFC, respectively, while pokeweed calli contained higher phenolic than flavonoid content. This is the first report describing chemical constituent screening and antioxidant activity of calli and other parts of the pokeweed plant. Results provided significant information to further enhance bioactive compound contents of pokeweed calli using elicitation methods.

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Optimization of Rice (Oryza sativa L.) ‘Tubtim Chumphae', for Callus Induction, Proliferation and Plantlet Regeneration

Taratima¹,

Plaikhunto²,

Wichachai³

et al. 2022

Asian J. of Plant Sciences

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Effects of Cultivation Media on In vitro Callus Induction and Regeneration Capabilities of Pakaumpuel Rice (Oryza sativa L.), Thai Rice Landrace

Cited by 5 publications

References 13 publications

Salinity Stress Response of Rice (Oryza sativa L. cv. Luem Pua) Calli and Seedlings

Salinity Stress Response of Rice (Oryza sativa L. cv. Luem Pua) Calli and Seedlings

Micropropagation of pokeweed (Phytolacca americana L.) and comparison of phenolic, flavonoid content, and antioxidant activity between pokeweed callus and other parts

Optimization of Rice (Oryza sativa L.) ‘Tubtim Chumphae', for Callus Induction, Proliferation and Plantlet Regeneration

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