Effects of Collagen-Derived Oligopeptide Prolylhydroxyproline on Differentiation of Mouse 3T3-L1 Preadipocytes

Abstract

Beneficial effects of collagen peptide ingestion to reduce serum triacylglycerides have been reported, suggesting that the physiological conditions of adipocytes are modulated following collagen peptide ingestion. In this study, the effects of prolylhydroxyproline, a major digestive product of ingested collagen peptide in the blood, on the differentiation of mouse 3T3-L1 preadipocytes in vitro were investigated. 3T3-L1 preadipocytes were induced to differentiate to adipocytes and treated with prolylhydroxyproline, or with an amino acid mixture of proline and hydroxyproline as a control. The amount of lipid was not affected by these treatments. However, the size of the lipid droplet was significantly smaller when treated with prolylhydroxyproline compared to the amino acid mixture or the non-treated control. Proton-coupled oligopeptide transporters were expressed in non-differentiated and/or differentiated 3T3-L1 cells. These results suggest that prolylhydroxyproline might modulate the morphology of lipid droplets by incorporation into adipocytes through the transporters.

Changes in composition and content of food‐derived peptide in human blood after daily ingestion of collagen hydrolysate for 4 weeks

“…In addition, we demonstrated that Pro‐Hyp and Hyp‐Gly stimulate the growth of mouse skin fibroblasts on collagen gels . Minaguchi et al . reported that Pro‐Hyp reduces the size of droplets in mouse 3 T3‐L1 preadipocytes.…”

Effects of Collagen Peptide Ingestion on Blood Lipids in Rats Fed a High-Lipid and High-Sucrose Diet

“…In addition, Pro-Hyp may affect TG metabolism, since it can modulate adipocyte differentiation. Specifically, the size of lipid droplets was found to be significantly smaller in differentiated mouse 3T3-L1 preadipocytes than in cells treated with a mixture of proline and hydroxyproline or in untreated cells (Minaguchi et al, 2012). However, further studies are required to elucidate the role of Pro-Hyp in blood lipid profiles.…”

Remodeling of rat stromal-vascular cells to brite/beige adipocytes by prolyl-hydroxyproline

“…Two hundred ng/ml Pro-Hyp was added to the medium every 2 days according to the method established by Minaguchi et al . [25]. The effects of Pro-Hyp on proliferation of cultured cells, morphology and number of LDs and mitochondria, and concentration of triglycerides in SVCs were evaluated on days 1, 3, 5, 7 and 8.…”

“…Pro-Hyp has been shown to stimulate the growth of mouse skin fibroblasts on collagen gels [37] and to modulate osteoarthritis induced by a high phosphorus diet in rat [27]. In addition, we previously reported that ingestion of Pro-Hyp alters the metabolic state of 3T3-L1 cells [25]. 3T3-L1 cells derived from mouse embryo fibroblasts undergo a highly conserved and efficient program of adipogenesis in culture and upon transplantation in vivo [17].…”