Effects of coconut milk alone or supplementation with pyridoxine in tris-extenders on viability of buck spermatozoa during vitrification

Daramola, J. O.; Adekunle, E.O.; Iyasere, Oluwaseun S.; Oke, O.E.; Sorongbe, T. A.; Iyanda, O. A.; Kehinde, A.R.; Aluko, S.P.; Olaoye, I. O.; Gbadebo, O.E.; Falolu, L.I.; Olukayode, E.O.; Ajayi, Richardson; Enikannaye, O.J.; Osunjaiye, E.D.

doi:10.1016/j.smallrumres.2016.02.004

Cited by 15 publications

(10 citation statements)

References 46 publications

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“…It was found that spermatozoa in semen extenders supplemented with vitamin B 6 during cryopreservation had higher motility compared with the control (Daramola et al, 2015(Daramola et al, , 2016. In vivo, a study conducted by Audet et al on young boars revealed that dietary supplements of water-soluble vitamins, including vitamin B 6 , improve sperm parameters, particularly sperm motility and count (Audet, Laforest, Martineau, & Matte, 2004).…”

Section: Discussionmentioning

confidence: 99%

Seminal plasma vitamin B ₆ levels in men with asthenozoospermia and men with normal sperm motility, a measurement using liquid chromatography with tandem mass spectrometry

Banihani

Aljabali

2020

Andrologia

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There is growing evidence that vitamin B6 has a valuable contribution in maintaining normal sperm parameters; however, this contribution has not yet well‐identified. Here, we aimed to measure the level of seminal plasma vitamin B6 in men with asthenozoospermia compared to men with normal sperm motility. Ninety‐seven human males with asthenozoospermia and eighty‐eight human males with normal sperm motility (control) were recruited in this study. Collected semen samples were assessed for sperm motility, sperm count and semen volume. Liquid chromatography with tandem mass spectrometry was used to measure seminal plasma vitamin B6 concentrations. A highly significant difference (p < .0001) in concentrations of seminal plasma vitamin B6 was found between asthenozoospermic and control groups. Besides, no statistical correlations were found between seminal plasma vitamin B6 level and sperm motility, sperm count, semen volume and men age in both tested groups. In conclusion, men with asthenozoospermia have lower seminal plasma vitamin B6 level compared to men with normal sperm motility. Also, seminal plasma vitamin B6 was found not to be correlated with sperm motility and count, semen volume and men age in both tested groups. These results may provide new contribution in the management of male infertility.

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Section: Discussionmentioning

confidence: 99%

Seminal plasma vitamin B ₆ levels in men with asthenozoospermia and men with normal sperm motility, a measurement using liquid chromatography with tandem mass spectrometry

Banihani

Aljabali

2020

Andrologia

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“…In this sense, further studies including a larger number of sperm parameters assessed as well as different sucrose concentrations in comparison with conventional freezing are needed. Previous studies were able to vitrify larger volumes of semen samples in human (Isachenko, Maettner, et al., ) and goat sperm (Daramola et al., ). Therefore, for a better application of this technique in the equine industry, it would be interesting to vitrify sperm in different packages with higher volume and sperm concentration.…”

Section: Discussionmentioning

confidence: 99%

“…A new aseptic technique packing sperm in sterile straws has been successfully developed for human (Isachenko, Maettner, et al., ; Sanchez et al., ; Slabbert, du Plessis, & Huyser, ) and goat (Daramola et al., ) sperm vitrification. The extenders used for sperm vitrification include carbohydrates, proteins and other non‐permeable substances.…”

Section: Introductionmentioning

confidence: 99%

Effect of different extenders for donkey sperm vitrification in straws

Díaz-Jiménez

Pereira

Ortiz

et al. 2017

Reprod Domestic Animals

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Contents Aseptic vitrification of semen samples packed in straws has been successfully developed in human but not in donkeys. The aim of this study was to compare the effect of two extenders for donkey sperm vitrification using straws. Ejaculates from four Andalusian donkeys were collected, and samples were extended in INRA‐96 (I) or Gent (G) supplemented with sucrose 0.25 M and 1% bovine serum albumin (BSA). Extended samples were cooled for one hour at 5°C. For vitrification, samples were filled in covered 0.25 ml straws and then plunged directly into liquid nitrogen. For warming, straws were immersed in INRA‐96 at 43°C. Results showed no significant differences between I and G treatments for TM (34.2% ± 8.7 vs. 30.7% ± 9.6) and PM (26.8% ± 7.3 vs. 24.6% ± 7.9), respectively. In conclusion, donkey sperm could be vitrified in straws either with INRA‐96 or with Gent in combination with sucrose and BSA.

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“…The sphere method has shown to protect sperm parameters after vitrification and warming in human (Isachenko et al, ), dog (Sánchez et al., ), fish (Merino et al., ), wild goat (Pradiee et al., ) and cat (Swanson et al, ). Likewise, vitrification in straws has obtained good results in human (Sanchez et al., ), domestic goat (Daramola et al., ) and donkeys (Diaz‐Jimenez et al., ). Even though both techniques have the same principle: an ultra‐rapid cooling rate of small volume samples (30–100 μl); results may be altered depending on the device used, as was previously showed for fish vitrification (Zilli et al., ).…”

Section: Introductionmentioning

confidence: 99%

Vitrification in straws conserves motility features better than spheres in donkey sperm

Díaz-Jiménez

Dorado

Pereira

et al. 2018

Reprod Domestic Animals

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Contents Sperm vitrification as alternative to conventional freezing is increasing in popularity in many species. It has been achieved by direct exposure of diluted semen to liquid nitrogen in spheres or straws. Both techniques have been successfully developed, but they had not been compared yet in donkeys. The aim of this study was to compare these two methods of vitrification for donkey semen. Ejaculates from six Andalusian donkeys were collected and extended in Gent without glycerol supplemented with sucrose 0.1 M (Molar). Samples were slowly cooled at 5°C. For vitrification, 30 μl suspensions (spheres) were dropped directly into liquid nitrogen (LN2) or filled in covered 0.25 ml straws and then plunged into the LN2 (straws). For warming, straws and spheres were directly immersed in 3 ml of INRA‐96 at 43°C. Total (TM, %) and progressive motility (PM, %) were objectively evaluated by computer‐assisted sperm analysis and plasma membrane integrity (PMI, %) by epifluorescence microscopy. Results showed the straw method resulted in significantly higher values than spheres for: TM (54.7% ± 10.1 vs. 28.6% ± 6.5) and PM (44.2% ± 9.4 vs. 17.7% ± 6.4), but no significant differences were found between straws or spheres for PMI (31.5 ± 10.7 vs. 41.6 ± 14.3) respectively. In conclusion, donkey sperm could be vitrified in straws obtaining better sperm motility parameters after warming in comparison to the sphere method.

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Effects of coconut milk alone or supplementation with pyridoxine in tris-extenders on viability of buck spermatozoa during vitrification

Cited by 15 publications

References 46 publications

Seminal plasma vitamin B ₆ levels in men with asthenozoospermia and men with normal sperm motility, a measurement using liquid chromatography with tandem mass spectrometry

Seminal plasma vitamin B ₆ levels in men with asthenozoospermia and men with normal sperm motility, a measurement using liquid chromatography with tandem mass spectrometry

Effect of different extenders for donkey sperm vitrification in straws

Vitrification in straws conserves motility features better than spheres in donkey sperm

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Effects of coconut milk alone or supplementation with pyridoxine in tris-extenders on viability of buck spermatozoa during vitrification

Cited by 15 publications

References 46 publications

Seminal plasma vitamin B 6 levels in men with asthenozoospermia and men with normal sperm motility, a measurement using liquid chromatography with tandem mass spectrometry

Seminal plasma vitamin B 6 levels in men with asthenozoospermia and men with normal sperm motility, a measurement using liquid chromatography with tandem mass spectrometry

Effect of different extenders for donkey sperm vitrification in straws

Vitrification in straws conserves motility features better than spheres in donkey sperm

Contact Info

Product

Resources

About

Seminal plasma vitamin B ₆ levels in men with asthenozoospermia and men with normal sperm motility, a measurement using liquid chromatography with tandem mass spectrometry

Seminal plasma vitamin B ₆ levels in men with asthenozoospermia and men with normal sperm motility, a measurement using liquid chromatography with tandem mass spectrometry