Effects of chronic estradiol or testosterone treatment upon sexual behavior in sexually sluggish male rats

Antonio-Cabrera, Edwards; Paredes, Raúl G.

doi:10.1016/j.pbb.2012.01.021

Cited by 21 publications

(9 citation statements)

References 46 publications

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“…In a previous study, male rats identified as SS after being tested five times with sexually receptive females were s.c. implanted with a silastic capsule containing either T or E2 and tested once a week for 12 weeks. Throughout the testing period, subjects displayed mounts and intromissions but did not ejaculate consistently [11], further supporting the observations that lower systemic T or E2 do not explain the lack of ejaculatory behavior in SS males [3,12,13].…”

Section: Introductionsupporting

confidence: 74%

“…The differences in the execution of the behavior between copulating (C, those that consistently ejaculate in less than 30 minutes) and SS male rats are not due to differences in plasma levels of testosterone (T) or estradiol (E 2 ) [3,4]. Moreover, chronic treatment with either hormone does not increase the execution of sexual behavior in SS males [11]. In a previous study, male rats identified as SS after being tested five times with sexually receptive females were s.c. implanted with a silastic capsule containing either T or E2 and tested once a week for 12 weeks.…”

Section: Introductionmentioning

confidence: 97%

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Steroid Receptors and Aromatase Gene Expression in Different Brain Areas of Copulating and Sexually Sluggish Male Rats

Antaramián

González‐Gallardo

García‐Ugalde

et al. 2015

The Journal of Sexual Medicine

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Introduction Sexually sluggish (SS) males have been identified in several species of mammals including rats. These animals take more than 30 minutes to ejaculate; they do not ejaculate or do so inconsistently despite being tested repeatedly with sexually receptive females. Different brain areas and hormones play an important role in the control of male sexual behavior. Aims Determine gene expression for the androgen receptor (AR), the estrogen receptor alpha (ERα), the progesterone receptor (PR), and the aromatase enzyme (ARO), in brain regions important in the control of male sexual behavior including the medial preoptic area (MPOA), the amygdala (AMG), the olfactory bulb (OB), and, as a control, the cortex (CTX) of copulating (C) and SS male rats. Methods Males that ejaculated within 30 minutes in three tests with receptive females were included in the C group, while those males that ejaculated in one or none of the four tests were included in the SS group. RNA was isolated 1 week after the last test of sexual behavior, and cDNA was synthesized from the brain areas listed above. Main Outcomes Measures Expression of the AR, ERα, PR, and ARO genes was determined by quantitative polymerase chain reaction (qPCR). Cyclophilin A (CycA) and tyrosine 3-monooxygenase-tryptophan activation protein zeta (Ywhaz) were housekeeping genes used to determine relative gene expression with the 2−ΔΔCt method. Results The expression of mRNA for AR and ARO increased in the MPOA of SS males. ARO mRNA was increased in the AMG of SS males. In the OB, ERα mRNA was increased and AR mRNA reduced in SS males. Conclusion These results indicate SS and C males show differences in gene expression within brain regions controlling sexual behavior.

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Section: Introductionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 97%

Steroid Receptors and Aromatase Gene Expression in Different Brain Areas of Copulating and Sexually Sluggish Male Rats

Antaramián

González‐Gallardo

García‐Ugalde

et al. 2015

The Journal of Sexual Medicine

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“…Males were s.c. implanted with a silastic capsule containing either testosterone or E 2 and tested once weekly, for 7 weeks. All subjects remained as sexually sluggish; they displayed mounts and intromissions but not a consistent ejaculatory pattern . Taken together, these results suggest that lower systemic testosterone or E 2 levels do not fully explain the alterations in sexual behaviour in sexually sluggish and NC males.…”

Section: Discussionmentioning

confidence: 78%

Testosterone or Oestradiol Implants in the Medial Preoptic Area Induce Mating in Noncopulating Male Rats

Antonio-Cabrera

Paredes

2014

J Neuroendocrinology

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Noncopulating (NC) male rats are those males that do not mount, intromit or ejaculate when repeatedly tested with receptive females. The lack of sexual behaviour in these males is not associated with alterations in testosterone or oestradiol (E2) plasma concentrations. Instead, it has been shown that androgen receptors are higher and oestrogen receptors are lower in the medial preoptic area (MPOA) of NC male rats than those observed in copulating (C) male rats. We have also observed reduced aromatase activity in the MPOA (but not in other brain regions) of NC male rats. The aim of the present study was to determine whether testosterone or E2 implants in the MPOA of NC male rats could induce sexual behaviour. Accordingly, in Experiment 1, we evaluated the long-term effects of testosterone or E2 implants in the MPOA, the ventromedial nucleus of the hypothalamus or the medial amygdala with respect to inducing sexual behaviour in castrated C male rats. Male rats were bilaterally implanted with a guide cannula, either empty or containing testosterone or E2. Starting 1 week later, all male rats were mated once weekly for 5 months. As described previously, the site where hormone implants most consistently induced sexual behaviour in castrated C male rats was the MPOA. Experiment 1 extended these findings showing that the males continued mating even 5 months after the implant. In the second experiment, NC males were implanted in the MPOA with a guide cannula empty or filled with testosterone or E2. One week after the testosterone or E2 implant, the percentage of males that mounted and intromitted started to increase and, 5 weeks after the implant, 50% of the subjects displayed mounts and intromissions. All NC males implanted with testosterone ejaculated consistently from week 11 after the implant until the end of testing (5 months), whereas all subjects implanted with E2 ejaculated from week 16 after the implant until the end of testing. These results support the hypothesis that, in the MPOA of NC male rats, there is a hormonal alteration associated with the lack of sexual behaviour.

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“…Water and commercial rat chow (RismartÒ) were provided ad libitum. All the experimental procedures were carried out according to the Official Mexican Norm for use and care of laboratory animals (NOM-062-ZOO-1999) [23] and the International Guiding Principles for Biomedical Research [24].…”

Section: Methodsmentioning

confidence: 99%

Stress During Puberty Facilitates Precancerous Prostate Lesions in Adult Rats

Herrera-Covarrubias¹,

Coria-Ávila²,

Hernández-Aguilar³

et al. 2017

Exp. Onc.

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Puberty can be a critical period for the long-term development of diseases, especially for stress-related disorders that depend on neuroendocrine and immune responses. Some organs like the prostate are prone to diseases that result from neuroendocrine or immune challenges, such as cancer. Aim: In the present study, we assessed the long-term effects of an acute pubertal stressor (immune-challenge) on the development of precancerous lesions in adult rats, and compared them with testosterone-induced prostatic lesions. Materials and Methods: Pubertal male rats received a single injection of lipopolysaccharide (LPS) or saline during puberty (5 weeks old). At adulthood (8 weeks old) males were subcutaneously implanted with either an empty capsule or filled with testosterone propionate (100 mg/kg). This resulted in a total of five groups: 1) intact untreated, 2) saline-treated and implanted with a blank capsule, 3) saline-treated and implanted with a testosterone capsule, 4) LPS-treated and implanted with a blank capsule, 5) LPS-treated and implanted with a testosterone capsule. Four weeks later, the rats were sacrified and their prostates processed for histology (hematoxylin and eosin stain) and blood serum processed for hormone analysis (testosterone and corticosterone). Results: Males treated with LPS (stressed during puberty via immune challenge) expressed epithelium dysplasia (specially in the ventral prostate), anisocytosis, presence of mononuclear cells, anisokariosis, non-basal polarity, abnormal nucleus-cytoplasm ratio, proplastic myoepithelium, and granular content in the lumen. These histological alterations were similar, but less severe than those observed in males implanted with testosterone during adulthood. Conclusion: These results indicate that pubertal exposure to an immune challenge (stress) facilitates the long-term development of prostatic lesions in adult male rats.

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Effects of chronic estradiol or testosterone treatment upon sexual behavior in sexually sluggish male rats

Cited by 21 publications

References 46 publications

Steroid Receptors and Aromatase Gene Expression in Different Brain Areas of Copulating and Sexually Sluggish Male Rats

Steroid Receptors and Aromatase Gene Expression in Different Brain Areas of Copulating and Sexually Sluggish Male Rats

Testosterone or Oestradiol Implants in the Medial Preoptic Area Induce Mating in Noncopulating Male Rats

Stress During Puberty Facilitates Precancerous Prostate Lesions in Adult Rats

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