Effects of chitin synthase double-stranded RNA on molting and oogenesis in the Chagas disease vector Rhodnius prolixus

Mansur, Juliana F.; Alvarenga, Evelyn S.L.; Figueira-Mansur, Janaina; Franco, Thiago A.; Ramos, Isabela; Masuda, Hatisaburo; Melo, Ana C.A.; Moreira, Mônica F.

doi:10.1016/j.ibmb.2013.12.006

Cited by 46 publications

(49 citation statements)

References 70 publications

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“…Mansur et al. also reported that CHS ‐RNAi treatment in Rhodnius prolixus adult females affected oogenesis, reducing the size of the ovary and resulting in a greater number of atresic oocytes compared with controls . This result is consistent with the findings of the present study, suggesting that MysCHSA plays an important role in R. prolixus ecdysis and fecundity, and further suggesting that MysCHSA is a good target for controlling the pest.…”

Section: Discussionsupporting

confidence: 91%

“…This study is the first report that identified the functions of MysCHSA in M. separata , as well as global changes at the protein and transcript levels after MysCHSA ‐RNAi treatment. Together, the data confirmed these observations and demonstrated that the MysCHSA silencing interfered with ecdysis and oogenesis . Therefore, we proposed a possible mechanism involving CHSA affecting ecdysis and the eggshell, via the canonical chitin biosynthesis pathway regulating the biosynthesis of chitin.…”

Section: Discussionsupporting

confidence: 81%

“…They belong to a large family of glycosyltransferases, with most insects possessing two different genes. CHS1/A is involved in the synthesis of chitin in the epidermis, trachea cuticle, and eggshell, while CHS2/B is expressed in the peritrophic matrix of the midgut . Notably in the CHS1/A gene, the use of alternate exons leads to the expression of different splice variants in Lepidopterans .…”

Section: Discussionmentioning

confidence: 99%

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Identification and Functional Analysis of Chitin Synthase A in Oriental Armyworm, Mythimna separata

et al. 2017

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Chitin synthases are very important enzymes for chitin synthesis in various species, which makes them a specific target of insecticides. In the present study, the function of the chitin synthase A (CHSA) gene isolated from Mythimna separate is investigated. The majority of dsMysCHSA treated larvae (89.50%) exhibit lethal phenotypes, including three phenotypes with severe cuticle deformations. The dsMysCHSA treatment in adult females affects oogenesis, and significantly reduce the ovary size and the oviposition number compared with controls. To determine how MysCHSA affects female fecundity, combined analyses of RNA-sequencing (RNA-Seq) transcriptome and TMT proteome (tandem mass tags) data in M. separata after treatment with MysCHSA-RNAi is performed. The differentially expressed proteins and genes affect fecundity-related proteins, energy metabolism, fatty acid metabolism, amino sugars, and nucleotide sugar metabolism pathways. Taken together, these results suggest that MysCHSA acts on M. separata ecdysis and fecundity, and has the potential as a target gene for pest control.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

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Identification and Functional Analysis of Chitin Synthase A in Oriental Armyworm, Mythimna separata

et al. 2017

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“…Injection of ds ChS s at an amount from 200 to 3000 ng per individual caused larval lethality, impaired ecdysis, decreased adult fecundity and/or affected egg hatching in Orthopteran Locusta migratoria 17, 22, Hemipteran Rhodnius prolixus 23 and Nilaparvata lugens 45, Coleopteran T. castaneum 7, 8, 21, Lepidopteran Spodoptera exigua 19, Ostrinia furnacalis 14 and Bombyx mori 46, 47, and Dipteran Drosophila melanogaster 48, 49 and Bactrocera dorsalis 24. These data demonstrate that ChS s are the potential candidate genes for an RNAi-based pest control strategy.…”

Section: Discussionmentioning

confidence: 99%

RNA interference of chitin synthase genes inhibits chitin biosynthesis and affects larval performance in Leptinotarsa decemlineata (Say)

Shi

Chen

et al. 2016

Int. J. Biol. Sci.

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Dietary introduction of bacterially expressed double-stranded RNA (dsRNA) has great potential for management of Leptinotarsa decemlineata. Identification of the most attractive candidate genes for RNA interference (RNAi) is the first step. In the present paper, three complete chitin synthase cDNA sequences (LdChSAa, LdChSAb and LdChSB) were cloned. LdChSAa and LdChSAb, two splicing variants of LdChSA gene, were highly expressed in ectodermally-derived epidermal cells forming epidermis, trachea, foregut and hindgut, whereas LdChSB was mainly transcribed in midgut cells. Feeding bacterially expressed dsChSA (derived from a common fragment of LdChSAa and LdChSAb), dsChSAa, dsChSAb and dsChSB in the second- and fourth-instar larvae specifically knocked down their target mRNAs. RNAi of LdChSAa+LdChSAb and LdChSAa lowered chitin contents in whole body and integument samples, and thinned tracheal taenidia. The resulting larvae failed to ecdyse, pupate, or emerge as adults. Comparably, knockdown of LdChSAb mainly affected pupal-adult molting. The LdChSAb RNAi pupae did not completely shed the old larval exuviae, which caused failure of adult emergence. In contrast, silencing of LdChSB significantly reduced foliage consumption, decreased chitin content in midgut sample, damaged midgut peritrophic matrix, and retarded larval growth. As a result, the development of the LdChSB RNAi hypomorphs was arrested. Our data reveal that these LdChSs are among the effective candidate genes for an RNAi-based control strategy against L. decemlineata.

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“…In the other hemipteran, Rhodnius prolixus , knocked down of crustacean cardioactive peptide by dsRNA had up to 84% mortality (Lee et al., ). Meanwhile, in this hemipteran insect, injection of chitin synthase dsRNA reduced transcript levels and oviposition, also caused reduction of ovary size, deformed eggs, and ecdysis abnormalities (Mansur et al., ). Ingestion of catalase dsRNA knocked down gene expression in S. avenae and reduced survival rate and ecdysis index (Deng and Zhao et al., ).…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of Cysteine and Trypsin Protease, Effect of Different Hosts on Protease Expression, and Rnai Mediated Silencing of Cysteine Protease Gene in the Sunn Pest

Amiri

Bandani

2015

Arch Insect Biochem Physiol

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Sunn pest, Eurygaster integriceps, is a serious pest of cereals in the wide area of the globe from Near and Middle East to East and South Europe and North Africa. This study described for the first time, identification of E. integriceps trypsin serine protease and cathepsin-L cysteine, transcripts involved in digestion, which might serve as targets for pest control management. A total of 478 and 500 base pair long putative trypsin and cysteine gene sequences were characterized and named Tryp and Cys, respectively. In addition, the tissue-specific relative gene expression levels of these genes as well as gluten hydrolase (Gl) were determined under different host kernels feeding conditions. Result showed that mRNA expression of Cys, Tryp, and Gl was significantly affected after feeding on various host plant species. Transcript levels of these genes were most abundant in the wheat-fed E. integriceps larvae compared to other hosts. The Cys transcript was detected exclusively in the gut, whereas the Gl and Tryp transcripts were detectable in both salivary glands and gut. Also possibility of Sunn pest gene silencing was studied by topical application of cysteine double-stranded RNA (dsRNA). The results indicated that topically applied dsRNA on fifth nymphal stage can penetrate the cuticle of the insect and induce RNA interference. The Cys gene mRNA transcript in the gut was reduced to 83.8% 2 days posttreatment. Also, it was found that dsRNA of Cys gene affected fifth nymphal stage development suggesting the involvement of this protease in the insect growth, development, and molting.

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Effects of chitin synthase double-stranded RNA on molting and oogenesis in the Chagas disease vector Rhodnius prolixus

Cited by 46 publications

References 70 publications

Identification and Functional Analysis of Chitin Synthase A in Oriental Armyworm, Mythimna separata

Identification and Functional Analysis of Chitin Synthase A in Oriental Armyworm, Mythimna separata

RNA interference of chitin synthase genes inhibits chitin biosynthesis and affects larval performance in Leptinotarsa decemlineata (Say)

Molecular Identification of Cysteine and Trypsin Protease, Effect of Different Hosts on Protease Expression, and Rnai Mediated Silencing of Cysteine Protease Gene in the Sunn Pest

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