Effects of Cellulose Crystallinity, Hemicellulose, and Lignin on the Enzymatic Hydrolysis of<i>Miscanthus sinensis</i>to Monosaccharides

Yoshida, Makoto; Liu, Yuan; Uchida, Satoshi; Kawarada, Kensuke; Ukagami, Yusuke; Ichinose, Hitomi; Kaneko, Satoshi; Fukuda, Kiyoharu

doi:10.1271/bbb.70689

Cited by 322 publications

(187 citation statements)

References 25 publications

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“…The sugar composition of the polysaccharides was analyzed as described previously (14,15). Briefly, the substrate was hydrolyzed with 2 M trifluoroacetic acid by incubating at 121°C for 1 h, and the acid was evaporated under a stream of N 2 gas.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, the substrate was hydrolyzed with 2 M trifluoroacetic acid by incubating at 121°C for 1 h, and the acid was evaporated under a stream of N 2 gas. The sugar composition of the sample was analyzed by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using a CarboPac TM PA20 column (Dionex Corp., Sunnyvale, CA) at a flow rate of 0.5 ml/min, as reported earlier (15).…”

Section: Methodsmentioning

confidence: 99%

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A β-l-Arabinopyranosidase from Streptomyces avermitilis Is a Novel Member of Glycoside Hydrolase Family 27

Ichinose

Fujimoto

Honda

et al. 2009

Journal of Biological Chemistry

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Arabinogalactan proteins (AGPs) are a family of plant cell surface proteoglycans and are considered to be involved in plant growth and development. Because AGPs are very complex molecules, glycoside hydrolases capable of degrading AGPs are powerful tools for analyses of the AGPs. We previously reported such enzymes from Streptomyces avermitilis. Recently, a ␤-Larabinopyranosidase was purified from the culture supernatant of the bacterium, and its corresponding gene was identified. The primary structure of the protein revealed that the catalytic module was highly similar to that of glycoside hydrolase family 27 (GH27) ␣-D-galactosidases. The recombinant protein was successfully expressed as a secreted 64-kDa protein using a Streptomyces expression system. The specific activity toward p-nitrophenyl-␤-L-arabinopyranoside was 18 mol of arabinose/min/mg, which was 67 times higher than that toward pnitrophenyl-␣-D-galactopyranoside. The enzyme could remove 0.1 and 45% L-arabinose from gum arabic or larch arabinogalactan, respectively. X-ray crystallographic analysis reveals that the protein had a GH27 catalytic domain, an antiparallel ␤-domain containing Greek key motifs, another antiparallel ␤-domain forming a jellyroll structure, and a carbohydrate-binding module family 13 domain. Comparison of the structure of this protein with that of ␣-D-galactosidase showed a single amino acid substitution (aspartic acid to glutamic acid) in the catalytic pocket of ␤-L-arabinopyranosidase, and a space for the hydroxymethyl group on the C-5 carbon of D-galactose bound to ␣-galactosidase was changed in ␤-L-arabinopyranosidase. Mutagenesis study revealed that the residue is critical for modulating the enzyme activity. This is the first report in which ␤-L-arabinopyranosidase is classified as a new member of the GH27 family.Arabinogalactan proteins (AGPs) 3 are a family of complex proteoglycans widely distributed in plants (1, 2). AGPs are also found in tree exudate gums and coniferous woods (3) and are characterized by the presence of large amounts of carbohydrate components rich in galactose (all the sugars in the present study are in the D-configuration unless otherwise specified) and L-arabinose and by protein components rich in hydroxyproline, serine, threonine, alanine, and glycine (4). Type II arabinogalactans and short oligosaccharides are the two types of carbohydrates attached to the AGP backbone. Type II arabinogalactans have ␤-1,3-linked galactosyl backbones in mono-or oligo-␤-1,6-galactosyl and/or L-arabinosyl side chains (2, 5). L-Arabinose and lesser amounts of other auxiliary sugars such as glucuronic acid, L-rhamnose, and L-fucose are attached to the side chains primarily at nonreducing termini (2). Molecular and biochemical evidence indicates that AGPs have specific functions during root formation, promotion of somatic embryogenesis, and attraction of pollen tubes to the style (6). However, because many putative protein cores exist and the structures of the carbohydrate moieties are complex, it has been difficult...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A β-l-Arabinopyranosidase from Streptomyces avermitilis Is a Novel Member of Glycoside Hydrolase Family 27

Ichinose

Fujimoto

Honda

et al. 2009

Journal of Biological Chemistry

Self Cite

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“…The enzymatic digestibility could be correlated with the total lignin content and the content of p-hydroxycinnamates. Yoshida et al (2008) indicated that lignin is the most significant resistance factor during enzymatic hydrolysis of normal M. sinensis, and some lignin might bind strongly to cellulose in chemically pretreated M. sinensis using the sodium chlorite delignification method, which inhibits cellulase activity. Our data showed that increasing of delignification led to increasing glucose yield in the enzymatic hydrolysis of AHP-treated Miscanthus.…”

Section: Enzymatic Digestibility Of Chemically Pretreated Miscanthusmentioning

confidence: 99%

Comparison of the enzymatic digestibility of physically and chemically pretreated selected line of diploid- Miscanthus sinensis Shiozuka and triploid- M. × giganteus

Hideno

Kawashima

Anzoua

et al. 2013

Bioresource Technology

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The diploid Miscanthus sinensis "Shiozuka" which was selected as a high-biomass producing line, and the triploid M. × giganteus (M×G) were treated by ball milling (physical treatment) and alkaline hydrogen peroxide treatment (AHP; chemical treatment), and their structural sugar compositions and enzymatic digestibility were compared. The structural sugar content of Shiozuka was moderate and lower than that of M×G. The Klason lignin content of Shiozuka was also lower than that of M×G.However, Shiozuka was sensitive to ball milling and AHP treatment; ball milled and AHP-treated Shiozuka had higher enzymatic digestibility than ball milled and AHPtreated M×G. Shiozuka would be promising feedstock to obtain fermentable sugars with low energy consumption. Finally, enzymes for the hydrolysis of chemically treated Miscanthus were isolated from Trichoderma reesei ATCC 66589 and Penicillium pinophilum. The sugar yield could be increased by enzymatic hydrolysis of AHP-treated samples with NaOH and H 2 O 2 and the isolated enzymes. Highlights> Miscanthus sinensis "Shiozuka" contained moderate sugar but low lignin contents.> Shiozuka was more sensitive to physical and chemical treatments than M×G. >Enzymatic digestibilities of the pretreated Shiozuka were higher than those of M×G.>Shiozuka is a promising bioenergy grass cultivar.3

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“…Such correlations are well known 25 and could be related to considerable adherence between cellulose and the noncellulosic timber portion. This results in the cellulose becoming obstructed during enzymatic hydrolysis, higher lignin content in the lignocellulosic matrix leading to stronger hydrolysis hindrance.…”

Section: 18mentioning

confidence: 83%

Processing Pine Wood into Vanillin and Glucose by Sequential Catalytic Oxidation and Enzymatic Hydrolysis

Tarabanko

Kaygorodov

Скиба

et al. 2016

Journal of Wood Chemistry and Technology

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Pine wood was processed into vanillin (up to 18 wt. % based on the lignin) and cellulose (typically 84-93 % of the initial amount in the wood) by one-stage catalytic oxidation, followed by enzymatic hydrolysis of the resulting cellulose into glucose (reducing sugar yield up to 70 % based on the cellulose). Correlation between the cellulose conversion in hydrolysis and the lignin 2 content in the post-oxidation lignocellulosic material was established, conforming to the general trend for the products of various delignification methods. The obtained results demonstrate the practical possibility of efficient two-step processing of wood into vanillin and glucose.

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Effects of Cellulose Crystallinity, Hemicellulose, and Lignin on the Enzymatic Hydrolysis ofMiscanthus sinensisto Monosaccharides

Cited by 322 publications

References 25 publications

A β-l-Arabinopyranosidase from Streptomyces avermitilis Is a Novel Member of Glycoside Hydrolase Family 27

A β-l-Arabinopyranosidase from Streptomyces avermitilis Is a Novel Member of Glycoside Hydrolase Family 27

Comparison of the enzymatic digestibility of physically and chemically pretreated selected line of diploid- Miscanthus sinensis Shiozuka and triploid- M. × giganteus

Processing Pine Wood into Vanillin and Glucose by Sequential Catalytic Oxidation and Enzymatic Hydrolysis

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