Effects of catalpol on bronchial asthma and its relationship with cytokines

Abstract

An animal (BALB/c mice) model of catalpol associated with bronchial asthma in vivo was established, and the effects of catalpol and its relationship with cytokines were investigated. A total of 30 adult BALB/c mice were randomly divided into a positive control group, a model group, and a catalpol group, with 10 mice in each group. The lung function of mice, the cell count, and the cytokine concentrations in bronchoalveolar lavage fluid (BALF) were detected.The levels of cytokines [interleukin 4 (IL-4), interleukin 5 (IL5), and interferon gamma (IFN-γ)] in BALF were measured with enzyme-linked immunosorbent assay methods. The total number of cells in the BALF of the group treated with catalpol was significantly lower than the model group. After treatment with catalpol, the eosinophils and neutrophils of the mice were remarkably reduced compared with the model group. The malondialdehyde content in the lung tissue homogenate of the mice was also decreased in the catalpol group. The cytokines IL-5 and IL-4 exhibited a similar tendency: the concentrations of IL-4 and IL-5 for the catalpol group were dramatically decreased compared with the model group. However, the IFN-γ concentration for the catalpol group was higher than the model group. The results indicated that IL-5 may involve in the pathologic process of asthma-like IL-4, and an inflammatory reaction may still exist in the airway during the remission stage of asthma. The imbalances of the cytokine network might be an important molecular basis in the asthma pathogenesis. It is suggested that catalpol may be a potential drug for the clinical treatment of asthma. K E Y W O R D S bronchial asthma, catalpol, IFN-γ, IL-4, IL-5 J Cell Biochem. 2019;120:8992-8998. wileyonlinelibrary.com/journal/jcb 8992 |

Multiple Biological Effects of an Iridoid Glucoside, Catalpol, and Its Underlying Molecular Mechanisms

“…Some of its anti-inflammatory potential has been also discussed later sections, for example in the context of neurodegenerative, cardiovascular disorders, cancer and in hepatoprotective effect (Section 3, Section 5, Section 6, and Section 7.1). The anti-inflammatory potential of catalpol had also been reported in acute pancreatitis, hyperhomocysteinemia, diabetic complications, acute lung injury, multiple sclerosis, and various lipopolysaccharide (LPS)-induced inflammatory models [9][10][11][12]. The anti-inflammatory and antioxidant effects of catalpol are summarized in Table 1.…”

Therapeutic Potential of Chungsangboha-tang for the Treatment of Asthma: A Review of Preclinical and Clinical Studies

“…Catalpol, a major component of Rehmanniae Radix, not only reduced transforming growth factor (TGF-β1) and epidermal growth factor (EGF), which are important factors in lung tissue damage and airway remodeling [ 52 ], but also lowered IL-4 and IL-5 and inhibited eosinophil infiltration and increase of CCL11, CCR3, and IL-5Rα in the OVA-induced asthma model [ 53 ]. In another study, catalpol showed immunomodulatory effects by reducing IL-4 and elevating decreased IFN-γ in BALF [ 54 ]. Corni Fructus has been reported to reduce IL-5, CCL11, and OVA-specific IgE and inhibit eosinophil infiltration in the OVA-induced asthma model [ 55 ].…”

The Herbal Cocktail GSYJ Attenuated Airway Inflammatory Cell Infiltration in a Chronic Asthmatic Mouse Model

“…In our recent study, we found that DFSG contains multiple compounds and exerts multitarget effects, as it regulates the cytokines IL-12, IFN-c, IL-5, and IL-13 through the coordinated action of its various compounds [29]. e isolated ingredients of medicinal plants include steroids, flavonoids, phenylpropanoids, and alkaloids, and compounds such as catalpol [30], β-sitosterol [31], chlorogenic acid [32], oleanolic acid [33], and ursolic acid [34] exert pharmacological antiasthma, immunomodulatory, and anti-inflammatory effects, as verified using in vitro and in vivo bioassays. erefore, we identified twenty-two different chemical constituents in GSYJ (data not shown).…”