Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane

Fornazier, Ricardo Francisco; Ferreira, Renato Rodrigues; Vitória, Ângela Pierre; Molina, Silvia; Lea, Peter J.; Azevedo, Ricardo Antunes

doi:10.1023/a:1015100624229

Cited by 131 publications

(29 citation statements)

References 47 publications

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“…Sugarcane leaves exposed to paraquat-induced oxidative stress also showed differences between the intensity of activity bands and total SOD activity, which can be explained by the lower sensitivity of the former method and/or the lack of detection of further isoforms [8]. In fact, seven Cu-Zn isoforms were found in sugarcane seedlings under the effect of cadmium, although SOD activity remained constant [18]. In GM, particularly MSOD but also CZSOD, were significantly up-regulated in vitro, with the transcript of MnSOD still increasing significantly thereafter (Fig.…”

Section: Discussionmentioning

confidence: 99%

Sugarcane (Saccharum sp. Hybrid) Propagated in Headspace Renovating Systems Shows Autotrophic Characteristics and Develops Improved Anti-oxidative Response

Aragón¹,

Carvalho²,

González

et al. 2008

Tropical Plant Biol.

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Previous results have shown that sugarcane plantlets micropropagated in Temporary Immersion Bioreactors (TIB) demonstrated a better morphology and physiological behaviour when compared to plantlets propagated in Gelled Medium (GM). The present work focuses on the onset of oxidative stress symptoms at transfer to ex vitro and during acclimatization. The specific ROS being produced were identified and tissue-located by infiltrating leaves with specific O 2 −. and H 2 O 2 staining dyes, respectively NBT and DAB. TIB plantlets showed trichomes stained with NBT and DAB, their density decreasing with time. Stomata were coloured with NBT and DAB in GM and, at the end of acclimatization, plantlets from both systems presented the lowest level of staining of both stomata and trichomes. The response of the anti-oxidative system was also analysed through in vitro and in gel enzyme activities and transcription levels of genes for key response enzymes. At the end of the in vitro phase, GM plantlets showed higher activities of APX and MDHAR, while CAT, GR, GT and DHAR activities increased in TIB. At the end of acclimatization SOD and CAT increased mainly in TIB, while GM induced the increase of APX. The immunobloting of peroxiredoxins showed that Prxs were expressed at higher levels in TIB plantlets, some showing polymerization. The transcription of genes coding for key response enzymes was strongly upregulated in GM plantlets. In conclusion and comparing with GM, TIB produced plantlets closer to autotrophy and with improved mobilization of the anti-oxidative response.

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Section: Discussionmentioning

confidence: 99%

Sugarcane (Saccharum sp. Hybrid) Propagated in Headspace Renovating Systems Shows Autotrophic Characteristics and Develops Improved Anti-oxidative Response

Aragón¹,

Carvalho²,

González

et al. 2008

Tropical Plant Biol.

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“…Acid phosphatase and alkaline phosphatase activity can be used as marker enzymes for Cd toxicity in plant as the enzymes have been shown to be sensitive to Cd. Also the toxicity of Cd has been related with the increase of lipid peroxidation and alterations in antioxidant systems in plants (Fornazier et al, 2002;Tran and Popova, 2013).…”

Section: Introductionmentioning

confidence: 99%

Biochemical effects of ethylene diamine tetra-acetic acid (EDTA) on cadmium treated maize (Zea mays L.) and cowpea (Vigna unguiculata L.)

Agbadah¹,

Nwachukwu²,

Okoh³

2016

Afr. J. Biotechnol.

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The effects of ethylenediaminetetra-acetic acid (EDTA) on germination, length of stem, area of leaf, fresh weight, level of lipid per oxidation, alkaline phosphatase, acid phosphatase, super oxide dismutase (SOD) and catalase in the roots of cadmium (Cd) treated maize (Zea mays L) and cowpea (Vigna unguiculata L) seedlings after 7 and 21 days of germination were determined in this study. The results obtained, indicate that at the end of 7 and 21 days of exposure to Cd, percentage germination of the seeds were not significantly different in both control and test soil (p>0.05). Morphological parameters (area of leaf, length of stem and fresh weight) were significantly reduced by Cd after 7 and 21 days. The supplementation of the soil sample with EDTA (0.5 mM or 1.0 mM) reversed the effect of Cd on these parameters as it significantly increased length of stems, area of leaf and plant fresh weight. There was a significant decrease in root acid phosphatase, root alkaline phosphatase, super-oxide dismutase (SOD) and catalase activity in both plant species. Both used doses of EDTA to ameliorate the above biochemical parameters. Increased level of root lipid peroxidation in Cd treated maize and cowpea seedlings was observed after 7 and 21 days of germination. Albeit, the level of lipid peroxidation in the root of Cd treated maize was significantly higher than that of cowpea, an indication that cowpea may be more tolerant than maize to Cd toxicity. The treatment of plant with, concentrations of EDTA (0.5 and 1.0 mM) failed to decrease the Cd induced, but increased the level of root lipid peroxidation. These results indicate that EDTA (0.5 mM and 1.0 mM) could be used for the treatment of Cd toxicity in plants; although, EDTA did not totally protect cowpea seedling from oxidative stress.

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“…In accordance to the results obtained with Trigonella sp. and sugar cane (Fornazier et al 2002;Niknam et al 2006), only one band of CAT activity was observed during PAGE analysis of the genotype CAB-6P. The CAT inactivation under saline conditions is attributed either to a possible modification of its biosynthesis (Feierabend and Dehne 1996) or to its photoinactivation (Polle 1997).…”

Section: Discussionmentioning

confidence: 99%

Effect of NaCl and CaCl2 on the antioxidant mechanism of leaves and stems of the rootstock CAB-6P (Prunus cerasus L.) under in vitro conditions

Chatzissavvidis

Veneti

Papadakis

et al. 2008

Plant Cell Tiss Organ Cult

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The effect of salinity on the non-enzymic and enzymic antioxidant activity, shoot proliferation and nutrient accumulation was studied in in vitro cultures of the rootstock CAB-6P (Prunus cerasus L.). Three concentrations (0, 30 and 60 mM) of NaCl or CaCl 2 were added to a modified MS medium. Between the two salt treatments used, only the explants treated with CaCl 2 presented significant decrease in growth parameters. The concentrations of Na + and Cl -in the explants treated with NaCl were increased, as NaCl in the culture medium increased. Furthermore, in the explants treated with CaCl 2

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Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane

Cited by 131 publications

References 47 publications

Sugarcane (Saccharum sp. Hybrid) Propagated in Headspace Renovating Systems Shows Autotrophic Characteristics and Develops Improved Anti-oxidative Response

Sugarcane (Saccharum sp. Hybrid) Propagated in Headspace Renovating Systems Shows Autotrophic Characteristics and Develops Improved Anti-oxidative Response

Biochemical effects of ethylene diamine tetra-acetic acid (EDTA) on cadmium treated maize (Zea mays L.) and cowpea (Vigna unguiculata L.)

Effect of NaCl and CaCl2 on the antioxidant mechanism of leaves and stems of the rootstock CAB-6P (Prunus cerasus L.) under in vitro conditions

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