Effects of Bacillus thuringiensis var. israelensis on the Black Fly Communities (Diptera, Simuliidae) in Tropical Streams

Docile, Tatiana Nascimento; Figueiró, Ronaldo; Molina, Óscar Sánchez; Gil-Azevedo, Leonardo H.; Nessimian, Jorge Luiz

doi:10.1007/s13744-020-00842-2

Cited by 5 publications

(2 citation statements)

References 45 publications

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“…Vector biocontrol strategies include the use of entomopathogenic bacteria, such as Bacillus spp. [4,5], and the use of mosquito densoviruses has also been considered [6,7]. In 1972, a Russian laboratory first identified a densovirus in Ae.…”

Section: Introductionmentioning

confidence: 99%

Recombinant Mosquito Densovirus with Bti Toxins Significantly Improves Pathogenicity against Aedes albopictus

Batool

Álam

Liu

et al. 2022

Toxins

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Mosquito densoviruses (MDVs) are mosquito-specific viruses that are recommended as mosquito bio-control agents. The MDV Aedes aegypti densovirus (AeDNV) is a good candidate for controlling mosquitoes. However, the slow activity restricts their widespread use for vector control. In this study, we introduced the Bacillus thuringiensis (Bti) toxin Cry11Aa domain II loop α8 and Cyt1Aa loop β6-αE peptides into the AeDNV genome to improve its mosquitocidal efficiency; protein expression was confirmed using nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS). Recombinant plasmids were transfected into mosquito C6/36 cell lines, and the expression of specific peptides was detected through RT-PCR. A toxicity bioassay against the first instar Aedes albopictus larvae revealed that the pathogenic activity of recombinant AeDNV was significantly higher and faster than the wild-type (wt) viruses, and mortality increased in a dose-dependent manner. The recombinant viruses were genetically stable and displayed growth phenotype and virus proliferation ability, similar to wild-type AeDNV. Our novel results offer further insights by combining two mosquitocidal pathogens to improve viral toxicity for mosquito control.

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Section: Introductionmentioning

confidence: 99%

Recombinant Mosquito Densovirus with Bti Toxins Significantly Improves Pathogenicity against Aedes albopictus

Batool

Álam

Liu

et al. 2022

Toxins

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“…Біопродукти на основі Bti є високоселективними з короткою стійкістю в навколишньому середовищі, і, отже, вони мають дуже низький потенціал завдати шкоди популяціям нецільових організмів. До сьогодні не було задокументовано жодного прикладу несподіваного розвитку патогенного організму в польових умовах [1]. Є певні ознаки того, що значне зниження біомаси комах може статися після тривалого використання Bti у прісноводних водно-болотних угіддях.…”

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Бактеріальне Зараження Личинок Мошок Та Використання Бакпрепаратів Для Контролю Чисельності Симуліїд

Сухомлін,

Зінченко,

Теплюк

et al. 2024

NCBio

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Considerable attention is paid to the biological method of controlling the number of blood-sucking insects, as it is highly selective, effective and environmentally safe. 340 reservoirs were examined within Volyn Polissia, among which 86 (14,9%) contained Simuliidae larvae with signs of bacterial lesion. Damaged larvae were recorded in small rivers and ameliorative canals, most of which, 71,5%, were mesosaprobic in terms of water quality. Bacterial infection was detected in the larvae of 10 species: S. pusillum, S. nigrum, S. equinum, S. erythrocephalum, S. ornatum, S. dolini, S. noelleri S. paramorsitans, S. promorsitans, S. morsitans. Оn Volyn Polissia a study was conducted on the effectiveness of reservoirs delarvation with three preparations based on Bacillus thuringiensis H14 bacteria - bactoculicide, bactolarvicide and BLP-2477. Experiments were carried out in ameliorative canals with a bed up to 2 m wide, 0.5 m deep and with a current speed of 0.7 m/s, which were densely populated by preimaginal phases of black flies. Treatment of the reservoir with preparations was carried out in two ways. The first consists in a one-time introduction of the entire dose, the second - in a gradual introduction of the dose over 6 and 24 hours. It was established that a single application of the entire dose of the preparation ensures only partial death (up to 75%) of black flies. An uneven death of larvae was registered in the entire area of treatment. The method of gradual introduction of the preparation, when it is washed out over 6 or 24 hours, is more effective (death of larvae up to 100%). To the quantity control of the black flies pre-imaginal phases development, preparations BLP-2477 and bactolarvicide are recommended as the most effective. The optimal dose for the death of black flies larvae at a distance of up to 1.5 km in ameliorative canals with width of up to 2 m, depth of 0.5 m and a flow speed of 0.7 m/s should be considered 400 g of the preparation. The deaths of non-target hydrobionts, caddisflies, mayflies, molluscs, and fishes were not registered. Key words: Simuliidae, Bacillus thuringiensis, reservoirs delarvation, bactoculicide, bactolarvicide, BLP-2477.

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The Biodiversity of Blackflies (Diptera: Simuliidae) in Brazil: New Distribution Records and Updated Checklist

Molina,

Aranda,

Chimes

2024

Neotrop Entomol

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Effects of Bacillus thuringiensis var. israelensis on the Black Fly Communities (Diptera, Simuliidae) in Tropical Streams

Cited by 5 publications

References 45 publications

Recombinant Mosquito Densovirus with Bti Toxins Significantly Improves Pathogenicity against Aedes albopictus

Recombinant Mosquito Densovirus with Bti Toxins Significantly Improves Pathogenicity against Aedes albopictus

Бактеріальне Зараження Личинок Мошок Та Використання Бакпрепаратів Для Контролю Чисельності Симуліїд

The Biodiversity of Blackflies (Diptera: Simuliidae) in Brazil: New Distribution Records and Updated Checklist

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