Effects of BA and Leaf Piece Orientation on Adventitious Bud Formation in Leaf Cutting of Begonia Tuberhybrida Group

Abstract

We studied the effects of the type of leaf cuttings, 6-benzylaminopurine (BA) application, and the section position and orientation of leaf pieces on adventitious bud formation in leaf cuttings inserted in rockwool beds, using expanded young leaf blades of Begonia Tuberhybrida Group 'Tenella'. In BA absence, 73% of whole leaf blades with petioles formed adventitious buds, whereas none of the 2 × 1.5 cm leaf pieces did at all. The percentages of surviving and adventitious bud formation of 2 × 1.5 cm leaf pieces without petioles were 47% and 0%, respectively. When these small leaf pieces were inserted in rockwool beds containing 0.25-2 ppm BA, the percentages of surviving and adventitious bud formation were 100% and 80%, respectively. In BA presence, the percentage of adventitious bud formation became lower from the base towards the tip of the leaf, and the leaf pieces at positions 2 cm away from the leaf bases did not form adventitious buds at all. When they were placed horizontally and vertically inverted, the percentages of adventitious bud formation were 60% and 80%, respectively.

Direct Shoot Bud Differentiation and Plantlet Regeneration from Leaf and Petiole Explants of Begonia tuberhybrida

“…This is particularly true for Begonia tuberhybrida. In fact, only limited data are available on low-frequency shoot regeneration and rooting from leaves and petioles (Debergh and Maene, 1981;Iida et al, 1986;Kiyokawa et al, 2001;Shimada et al, 2007;Viseur and Lievens, 1987). Also, micropropagation of Begonia using microshoots has limited scaleup potential.…”

Cultivo in vitro de pétalos de cuatro variedades de Begonia elatior

“…For the development of this research, micropropagation was used as a tool for the massive propagation of Begonia elatior. For the development of the research, the petal was chosen as explant because although the begonias have been studied by several researchers (Takayama and Misawa,1982;Simmonds and Werry, 1987;Bowes and Curtis, 1991;Nakano et al, 1999;Bouman and Klerk, 2001;Kishimoto et al, 2002;Burritt and Leung, 2003;Espino et al, 2004;Nhut et al, 2005;Shimada et al, 2006Shimada et al, , 2007Mendi et al, 2009;Romocea et al, 2010;Kabirnataj et al, 2012). There is little or no information about the in vitro cultivation of petal of Begonia elatior as mother material, so we think that this type of explant can be interesting and cheap, especially because of the amount of flowers and petals that one has in a plant.…”