Effects of atrazine on Ochrobactrum anthropi membrane fatty acids

Laura, D.; Socio, G De; Frassanito, Rita; Rotilio, Domenico

doi:10.1128/aem.62.7.2644-2646.1996

Cited by 44 publications

(31 citation statements)

References 12 publications

(14 reference statements)

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“…Exposure to DEHP did not have this effect at either concentration. The increase in saturation was similar to that reported for Pseudomonas putida P8, and Ochrobactrum anthropi, after exposure to known membrane-disrupting compounds and is indicative of an increase in the ordering of membrane lipids to reduce fluidity [26,37]. The impact of DEP and DEHP on membrane composition was in agreement with predictions based on the lipophilic characteristics of both phthalates.…”

Section: Resultssupporting

confidence: 84%

“…Such changes are indicative of membrane disruption, and have been reported as markers of environmental stress [23,24]. However, although reported for phenolic compounds such as phenol [25] and the s-triazine herbicide atrazine [26], membrane disruption has not been associated directly with phthalates [17]. Published K ow coefficients are 2.38 for diethyl phthalate (DEP) and 7.50 for di (2-ethyl hexyl) phthalate (DEHP) [5], indicating that of the two, only DEP will be membrane disruptive.…”

Section: Introductionmentioning

confidence: 99%

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Degradation and Impact of Phthalate Plasticizers on Soil Microbial Communities

Cartwright

Thompson²,

Burns

2000

Environ Toxicol Chem

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To assess the impact of phthalates on soil microorganisms and to supplement the environmental risk assessment for these xenobiotics, soil was treated with diethyl phthalate (DEP) or di (2-ethyl hexyl) phthalate (DEHP) at 0.1 to 100 mg/g. Bioavailability and membrane disruption were proposed as the characteristics responsible for the observed fate and toxicity of both compounds. Diethyl phthalate was biodegraded rapidly in soil with a half-life (t 50 deg) of 0.75 d at 20ЊC, and was not expected to persist in the environment. The DEHP, although biodegradable in aqueous solution (t 50 deg Ͻ 15 d at 20ЊC), was recalcitrant in soil, because of poor bioavailability (only 10% degraded by 70 d at 20ЊC) and was predicted to account for the majority of phthalate contamination in the environment. Addition of DEP or DEHP to soil at a concentration similar to that detected in nonindustrial environments (0.1 mg/g) had no impact on the structural diversity (bacterial numbers, fatty acid methyl ester analysis) or functional diversity (BIOLOG) of the microbial community. At concentrations representative of a phthalate spill, DEP (Ͼ1 mg/g) reduced numbers of both total culturable bacteria (by 47%) and pseudomonads (by 62%) within 1 d. This was due to disruption of membrane fluidity by the lipophilic phthalate, a mechanism not previously attributed to phthalates. However, DEHP had no effect on the microbial community or membrane fluidity, even at 100 mg/g, and was predicted to have no impact on microbial communities in the environment.

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Section: Resultssupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Degradation and Impact of Phthalate Plasticizers on Soil Microbial Communities

Cartwright

Thompson²,

Burns

2000

Environ Toxicol Chem

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“…The microorganism was isolated from activated sludge contaminated with atrazine. Culture morphology and biochemical reaction in the API ID 32 E and ID 32 GN (Biome èrieux) system were consistent with the identi¢cation of O. anthropi [10].…”

Section: Microorganism Isolation and Identi¢cationsupporting

confidence: 66%

“…For this reason it is important to understand the structural, kinetic and immunological properties of the glutathione transferases of a wide number of bacterial strains. Here we report the puri¢cation and N-terminal sequence of a novel glutathione transferase from O. anthropi, a bacterium able to degrade atrazine [10]. We have studied the immunological properties of the enzyme of O. anthropi and compared the N-terminal sequence with known glutathione transferases puri¢ed from other bacterial strains.…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of a novel glutathione transferase from Ochrobactrum anthropi

Favaloro¹

1998

FEMS Microbiology Letters

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Glutathione transferase was purified from Ochrobactrum anthropi and its N-terminal sequence was determined to be MKLYYKVGACSLAPHIILSEAGLPY. The apparent molecular mass of the protein (24 kDa) was determined by SDSpolyacrylamide gel electrophoresis analysis. The amino acid sequence obtained showed similarities with known bacterial glutathione transferases in the range of 72^64%. Immunoblotting experiments performed with antisera raised against glutathione transferase from O. anthropi did not show cross-reactivity with two bacterial glutathione transferases belonging to Serratia marcescens and Proteus mirabilis. z 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.The amino acid sequence reported in this paper has been submitted to the EMBL Data Bank with accession number P81065. FEMS Microbiology Letters 160 (1998) 81^86

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“…These include herbicides, hemicellulose, anthracene, nicotine, phenol and other complex organic molecules including polycyclic aromatic hydrocarbons and crude oil. Ochrobactrum strains accumulate heavy metal ions and express degrading properties towards multiple antibiotics (Asano et al, 1992;van den Tweel et al, 1993;Laura et al, 1996;Sonke et al, 2005;Qiu et al, 2006). In our previous study, we found that Ochrobactrum sp.…”

Section: Introductionmentioning

confidence: 98%

Inactivation of AHLs by Ochrobactrum sp. A44 depends on the activity of a novel class of AHL acylase

Czajkowski

Krzyżanowska

Karczewska

et al. 2011

Environ Microbiol Rep

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The soil isolate Ochrobactrum sp. A44 inactivates N-acyl homoserine lactone (AHL) quorum sensing signal molecules and is capable of quenching the AHL-dependent virulence of Pectobacterium carotovorum in planta. To characterize this AHL inactivating activity, Ochrobactrum cell extracts were prepared and their capacity to degrade a broad range of AHLs was determined. AHLs with acyl chains ranging from C4 to C14 with or without 3-oxo or 3-hydroxy substituents were all inactivated to varying extents; long chain AHLs were generally more susceptible than short chain compounds irrespective of the three position substituent. HPLC and LC-tandem mass spectrometry of the AHL degradation products revealed that the AHL inactivating activity present in the Ochrobactrum cell extract cleaved the AHL amide bond. To identify the gene(s) responsible for AHL degradation, Ochrobactrum sp. A44 was subjected to random transposon (Tn) mutagenesis and the resulting mutants screened for the loss of AHL acylase activity. The Tn insertion in mutant A6731 was mapped to a gene termed aiiO, the translated product of which belongs to the α/β hydrolase superfamily which constitutes a novel type of AHL acylase.

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Effects of atrazine on Ochrobactrum anthropi membrane fatty acids

Cited by 44 publications

References 12 publications

Degradation and Impact of Phthalate Plasticizers on Soil Microbial Communities

Degradation and Impact of Phthalate Plasticizers on Soil Microbial Communities

Purification and characterization of a novel glutathione transferase from Ochrobactrum anthropi

Inactivation of AHLs by Ochrobactrum sp. A44 depends on the activity of a novel class of AHL acylase

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