Effects of arbuscular mycorrhizal fungi on <i>Leymus chinensis</i> seedlings under salt–alkali stress and nitrogen deposition conditions: from osmotic adjustment and ion balance

Lin, Jixiang; Peng, Xiaoyuan; Hua, Xiaoyu; Sun, Shengnan; Wang, Yingnan

doi:10.1039/c8ra00721g

Cited by 25 publications

(23 citation statements)

References 41 publications

(40 reference statements)

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“…Plants can accumulate osmotic adjustment substances, which is an effective way to alleviate osmotic stress cause by AS (Lin et al, ). Many of these osmotic adjustment substances are N ‐containing compounds, such as amino acids, amides, and betaines.…”

Section: Discussionmentioning

confidence: 99%

“…In the northeast of China, alkaline meadows cover >70% of the land, and the area continues to expand (Lin, Wang, Sun, Mu, & Yan, 2017). Alkaline stress (NaHCO 3 or Na 2 CO 3 ) is more severe than saline stress due to its unique high pH, which can inhibit ion uptake and disrupt the ionic balance of plant cells (Lin et al, 2018;Yang, Zheng, Tian, Wu, & Zhou, 2011). However, some halophytes in northern China, including Puccinellia tenuiflora, Leymus chinesis, and Suaeda glauca, have evolved various regulatory and metabolic mechanisms and can naturally survive in alkali-degraded soil.…”

Section: Introductionmentioning

confidence: 99%

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Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

et al. 2019

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Alkali soil is a major abiotic constraint that limits plant distribution and yield in the northeast of China. Puccinellia tenuiflora is considered the most promising grass species for salt‐alkali grassland restoration. However, there is little information on the molecular mechanisms underlying how arbuscular mycorrhizal fungi (AMF) enhances P. tenuiflora stress responses in alkali‐degraded soil. In this study, AMF colonization, growth, photosynthetic pigments, and inorganic ion contents were measured. Isobaric tags for relative and absolute quantification‐based quantitative proteomic technology were employed to identify the differentially abundant proteins in P. tenuiflora seedlings with or without AMF under alkalinity stress. The results showed that AMF colonization increased seedling biomass, photosynthetic pigment contents, and K+/Na+ ratio under alkalinity stress. Moreover, a total of 598 proteins were significantly differentially regulated in P. tenuiflora seedlings after AMF inoculation under alkalinity stress compared with under alkalinity stress alone. The results showed that AMF inoculation significantly improved protein synthesis, reactive oxygen species scavenging, and nitrogen metabolism to promote the biosynthesis of osmotic substances in response to alkalinity stress. In addition, P. tenuiflora seedlings produced more energy to compensate for the energy loss caused by alkalinity stress after AMF inoculation. In conclusion, these findings provide new insights into the physiological mechanisms of the response to alkali‐degraded soil in P. tenuiflora seedlings with AMF and also clarify the role of AMF in the molecular regulation network of P. tenuiflora under alkalinity stress.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

et al. 2019

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“…Previous studies have demonstrated that alkaline salt stress and neutral salt (e.g., NaCl and Na 2 SO 4 ) stress are actually two entirely different stress types [5][6][7]. Alkaline stress not only has the same inhibitory effects as saline stress but also has the unique influence of high pH, which inhibits ion uptake and disrupts the ionic balance of plant cells [8,9]. However, to the best of our knowledge, the majority of studies have emphasized the toxic effects of salt stress and paid little attention to alkaline stress, and the specific alkali-tolerance mechanisms of halophytes are still not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Metabolomics Analysis Reveals the Alkali Tolerance Mechanism in Puccinellia tenuiflora Plants Inoculated with Arbuscular Mycorrhizal Fungi

et al. 2020

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Soil alkalization is a major environmental threat that affects plant distribution and yield in northeastern China. Puccinellia tenuiflora is an alkali-tolerant grass species that is used for salt-alkali grassland restoration. However, little is known about the molecular mechanisms by which arbuscular mycorrhizal fungi (AMF) enhance P. tenuiflora responses to alkali stress. Here, metabolite profiling in P. tenuiflora seedlings with or without arbuscular mycorrhizal fungi (AMF) under alkali stress was conducted using liquid chromatography combined with time-of-flight mass spectrometry (LC/TOF-MS). The results showed that AMF colonization increased seedling biomass under alkali stress. In addition, principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) demonstrated that non-AM and AM seedlings showed different responses under alkali stress. A heat map analysis showed that the levels of 88 metabolites were significantly changed in non-AM seedlings, but those of only 31 metabolites were significantly changed in AM seedlings. Moreover, the levels of a total of 62 metabolites were significantly changed in P. tenuiflora seedlings after AMF inoculation. The results suggested that AMF inoculation significantly increased amino acid, organic acid, flavonoid and sterol contents to improve osmotic adjustment and maintain cell membrane stability under alkali stress. P. tenuiflora seedlings after AMF inoculation produced more plant hormones (salicylic acid and abscisic acid) than the non-AM seedlings, probably to enhance the antioxidant system and facilitate ion balance under stress conditions. In conclusion, these findings provide new insights into the metabolic mechanisms of P. tenuiflora seedlings with arbuscular mycorrhizal fungi under alkali conditions and clarify the role of AM in the molecular regulation of this species under alkali stress.

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“…Sodium is one of the most dominating toxic ions that induce soil salinization. Extremely accumulated Na can induce ionic imbalance and disrupt normal physiological metabolisms ( Lin et al, 2018 ; He et al, 2020 ). Our observations showed that Na was significantly accumulated in plants in both S. salsa community and P. tenuiflora community, indicating that these plants can survive from excess Na from soils.…”

Section: Discussionmentioning

confidence: 99%

Ionomic and Metabolomic Analyses Reveal Different Response Mechanisms to Saline–Alkali Stress Between Suaeda salsa Community and Puccinellia tenuiflora Community

et al. 2021

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Soil salinization imposes severe stress to plants, inhibits plant growth, and severely limits agricultural productivity and land utilization. The response of a single plant to saline-alkali stress has been well investigated. However, the plant community that usually works as a group to defend against saline–alkali stress was neglected. To determine the functions of plant community, in our current work, Suaeda salsa (S. salsa) community and Puccinellia tenuiflora (P. tenuiflora) community, two communities that are widely distributed in Hulun Buir Grassland in Northeastern China, were selected as research objects. Ionomic and metabolomic were applied to compare the differences between S. salsa community and P. tenuiflora community from the aspects of ion transport and phenolic compound accumulation, respectively. Ionomic studies demonstrated that many macroelements, including potassium (K) and calcium (Ca), were highly accumulated in S. salsa community whereas microelement manganese (Mn) was highly accumulated in P. tenuiflora community. In S. salsa community, transportation of K to aboveground parts of plants helps to maintain high K+ and low Na+ concentrations whereas the accumulation of Ca triggers the salt overly sensitive (SOS)-Na+ system to efflux Na+. In P. tenuiflora community, enrichment of Mn in roots elevates the level of Mn-superoxide dismutase (SOD) and increases the resistance to saline–alkali stress. Metabolomic studies revealed the high levels of C6C1-compounds and C6C3C6-compounds in S. salsa community and also the high levels of C6C3-compounds in P. tenuiflora community. C6C1-compounds function as signaling molecules to defend against stress and may stimulate the accumulation of C6C3C6-compounds. C6C3-compounds contribute to the elimination of free radicals and the maintenance of cell morphology. Collectively, our findings determine the abundance of phenolic compounds and various elements in S. salsa community and P. tenuiflora community in Hulun Buir Grassland and we explored different responses of S. salsa community and P. tenuiflora community to cope with saline–alkali stress. Understanding of plant response strategies from the perspective of community teamwork may provide a feasible and novel way to transform salinization land.

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Effects of arbuscular mycorrhizal fungi on Leymus chinensis seedlings under salt–alkali stress and nitrogen deposition conditions: from osmotic adjustment and ion balance

Abstract: We evaluated the contribution of arbuscular mycorrhizal fungi to the growth, ion content, and solute accumulation of Leymus chinensis seedlings under salt–alkali stress and nitrogen deposition.

Cited by 25 publications

References 41 publications

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

Metabolomics Analysis Reveals the Alkali Tolerance Mechanism in Puccinellia tenuiflora Plants Inoculated with Arbuscular Mycorrhizal Fungi

Ionomic and Metabolomic Analyses Reveal Different Response Mechanisms to Saline–Alkali Stress Between Suaeda salsa Community and Puccinellia tenuiflora Community

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