Effects of angiogenic factors and 3D-microenvironments on vascularization within sandwich cultures

Nishiguchi, Akihiro; Matsusaki, Michiya; Asano, Yoshiya; Shimoda, Hiroshi; Akashi, Mitsuru

doi:10.1016/j.biomaterials.2014.01.079

Cited by 85 publications

(84 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Endothelium grown in 3D conditions by the use of matrigel or hydrogel substrates displays the typical ability to form interconnected tubules and its gene expression and cell signaling are clearly different from the monolayer cultures (Blobel, 2010;Engelse, Laurens, Verloop, Koolwijk, & van Hinsbergh, 2008;Hofmann et al, 2008;X. Ma et al, 2013;Nishiguchi, Matsusaki, Asano, Shimoda, & Akashi, 2014;Pedersen et al, 2013b).…”

Section: Critical Concernsmentioning

confidence: 99%

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

et al. 2015

View full text Add to dashboard Cite

Bone plays several physiological functions and is the second most commonly transplanted tissue after blood. Since the treatment of large bone defects is still unsatisfactory, researchers have endeavoured to obtain scaffolds able to release growth and differentiation factors for mesenchimal stem cells, osteoblasts and endothelial cells in order to obtain faster mineralization and prompt a reliable vascularization.Nowadays, the application of osteoblastic cultures spans from cell physiology and pharmacology to cytocompatibility measurement and osteogenic potential evaluation of novel biomaterials. To overcome the simple traditional monocultures in vitro, co-cultures of osteogenic and vasculogenic precursors were introduced with very interesting results. Increasingly complex culture systems have been developed, where cells are seeded on proper scaffolds and stimulated so as to mimic the physiological conditions more accurately. These bioreactors aim at enabling bone regeneration by incorporating different cells types into bioinspired materials within a surveilled habitat.This review is focused on the most recent developments in the organomimetic cultures of osteoblasts and vascular endothelial cells for bone tissue engineering.

show abstract

Section: Critical Concernsmentioning

confidence: 99%

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Early osteoblastic and 24 endothelial differentiations were observed and cell pro- 25 liferation was increased after 7 days of culture. In 3D, cell 26 migration was observed between layers of LBL constructs, 27 as well as an osteoblastic differentiation. These results 28 indicate that LBL assembly of PLA layers could be suitable 29 …”

Section: Tissue Engineering Constructs and Cell Substratesmentioning

confidence: 99%

Layer-by-layer bioassembly of cellularized polylactic acid porous membranes for bone tissue engineering

Gudurić

Metz

Siadous

et al. 2017

J Mater Sci: Mater Med

View full text Add to dashboard Cite

“…We observed that both the expression of angiogenic factors from surrounding NHDFs and effect of 3D microenvironments affected the vascularization by sandwich cultures. 83 Therefore, such blood capillaries with a lumen have enormous potential for drug permeability testing or tumor angiogenesis and metastasis assays.…”

Section: Cell Accumulation Technique For Vascularized 3d Tissue Constmentioning

confidence: 99%

Control of extracellular microenvironments using polymer/protein nanofilms for the development of three-dimensional human tissue chips

Matsusaki

Akashi

2014

Polym J

Self Cite

View full text Add to dashboard Cite

Cell functions, such as adhesion, migration, proliferation, differentiation, organization, protein expression and death, are dynamically regulated at the surface of cell membranes by the interaction between a ligand molecule of the cellular microenvironments and the membrane protein receptors. Furthermore, physical signals of cellular microenvironments, stiffness, elasticity and hydration, are also important factors for the modulation of cellular functions. In the body, natural extracellular matrices (ECMs) and ECM-bound growth factors regulate cell functions. The universal control of cell functions at the single-cell level through the artificial modulation of extracellular microenvironments by mimicking the natural ECMs would be a key technique for biomedical fields. In the present review, the fabrication of nanometer-sized artificial ECM films using various polymers and proteins on single-cell surfaces to control cell functions is described. The thickness, charge and component of the ECM films were observed to significantly affect the cell functions. Moreover, the optimized ECM films induced cell-cell organization to construct three-dimensional human tissue chips. Control of extracellular microenvironments using a nanometersized polymer/protein film as an artificial ECM would be useful as a novel technique to regulate cell functions as desired. INTRODUCTIONTissue cells in the body reside in the micrometer-sized fibrous meshwork of the extracellular matrix (ECM). Tissue dynamics, such as formation, function and regeneration after damage, are the result of an intricate temporal and spatial coordination of numerous individual cell fate processes, each of which is induced by a myriad of signals originating from the extracellular microenvironment (Scheme 1). 1 The extracellular microenvironment is a hydrated protein and proteoglycan-based ECM gel network comprising soluble and physically bound signals as well as signals arising from cell-cell interactions. 2 The ECM is typically composed of fibronectin (FN), collagen and laminin and provides complex biochemical and physical signals. 3,4 The ECM not only stores growth factors and cytokines but also induces cell-cell contacts and cell-matrix interactions. 2 Accordingly, control of the cellular microenvironment using artificial ECM and growth factors will be an important in vitro technique to control cell growth, cytokine expression, stem cell differentiation and extracellular assembly. To control the cellular microenvironment, the patterning of substrate surfaces 5 and the chemical modifications of cell surfaces 6-8 have been generally used. However, substrate modification cannot modify the important cell apical membrane, and chemical modifications have limitations because of cytotoxicity, complicated methodology or less application.

show abstract

Effects of angiogenic factors and 3D-microenvironments on vascularization within sandwich cultures

Cited by 85 publications

References 34 publications

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

Layer-by-layer bioassembly of cellularized polylactic acid porous membranes for bone tissue engineering

Control of extracellular microenvironments using polymer/protein nanofilms for the development of three-dimensional human tissue chips

Contact Info

Product

Resources

About