Effects of aerobic exercise on the inflammatory cytokine profile and expression of lipolytic and thermogenic genes in β1-AR−/− mice adipose tissue

Rodrigues, Aurora Corrêa; Leal, Tiago Ferreira; Costa, A. C.; Silva, Franciany de Jesus; Soares, Leôncio Lopes; Brum, Patrícia Chakur; Hermsdorff, Helen Hermana Miranda; Pelúzio, Maria do Carmo Gouveia; Prímola-Gomes, Thales Nicolau; Natali, Antônio José

doi:10.1016/j.lfs.2019.02.031

Cited by 9 publications

(5 citation statements)

References 39 publications

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“…Additionally, no significant change was observed in Adrb3 expression in HFD-AT group as compared to both HFD-OW and control groups. This finding was similar to that in a previous work by Rodrigues et al who reported that endurance training for eight weeks did not change in Adrb3 gene expression in WAT of mice (38). However, there are a few studies that reported an increase in Adrb3 expression following endurance training in WAT of rats that had a normal (21) and HFD (20).…”

Section: Discussionsupporting

confidence: 91%

The effect of aerobic exercise training on gene expression of beta3-adrenergic receptor and beta-arrestin2 in inguinal white adipose tissue of mice fed with a high fat diet

Daneshyar

Bahmani

Fourotan

2021

J Shahrekord Univ Med Sci

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Background and aims: Beta-adrenergic signaling deficiency has been established to be related to obesity and related diseases. Beta3- adrenergic receptor (Adrb3) and beta-arrestin2 (Barr2) are pivotal agents in the beta-adrenergic-signaling pathway. This study aimed to investigate the preventive effect of aerobic training on dysregulation of Adrb3 and Barr2 gene expression that was induced by high-fat diet (HFD) in inguinal white adipose tissue of mice. Materials and Methods: Twenty-one C57BL/6 mice were assigned to three groups as follows: 1) control group (n=7), 2) high-fat diet-induced overweight (HFD-OW) (n=7), and 3) high-fat diet with aerobic training (HFD-AT) (n=7). The HFD-OW group were fed with a HFD for 12 weeks. The HFD-AT group had aerobic training for six weeks on a treadmill in addition to feeding with the HFD. The real-time polymerase chain reaction (PCR) method was used to measure the gene expression of Adrb3 and Barr2 in inguinal white adipose tissue. Results: The gene expression of Adrb3 did not significantly change between groups (P>0.05). However, the expression of Barr2 in HFD-OW group was significantly increased as compared to the control group (1.5-fold: P=0.001). Interestingly, the Barr2 expression in HFD-AT group was significantly lower compared with HFD-OW group (P=0.045). Conclusion: The results indicated that aerobic training could inhibit the upregulation of Barr2 induced by HFD. It seems that a portion of the preventive effect of aerobic training on the development of obesity may be mediated by inhibiting the Barr2 expression in adipose tissue.

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Section: Discussionsupporting

confidence: 91%

The effect of aerobic exercise training on gene expression of beta3-adrenergic receptor and beta-arrestin2 in inguinal white adipose tissue of mice fed with a high fat diet

Daneshyar

Bahmani

Fourotan

2021

J Shahrekord Univ Med Sci

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“…Our current data show that long-term exercise was able to counteract the iWAT inflammation that accompanies obesity and aging, as revealed by: i) the lower macrophages content (F4/80 + /Cd11b + ) in iWAT SVF; ii) the decrease observed in proinflammatory cytokines ( Tnf , Il6) ; and iii) the increase in antiinflammatory adipocytokines Adiponectin ( Adipoq) and Il4 mRNA levels. Similar antiinflammatory effects have been widely described in the current literature for different types of exercise in the iWAT and visceral WAT depots of adult DIO animals [ 50 , 70 , 74 , 90 ]. Concerning the mechanisms by which exercise could promote beige adipocytes markers in iWAT, the observed local increases in adiponectin and IL-4 could be involved, since both have been shown to promote browning of subcutaneous WAT by inducing proliferation of M2 macrophages [ 35 , 63 ].…”

Section: Discussionsupporting

confidence: 77%

Differential remodeling of subcutaneous white and interscapular brown adipose tissue by long-term exercise training in aged obese female mice

et al. 2023

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Obesity exacerbates aging-induced adipose tissue dysfunction. This study aimed to investigate the effects of long-term exercise on inguinal white adipose tissue (iWAT) and interscapular brown adipose tissue (iBAT) of aged obese mice. Two-month-old female mice received a high-fat diet for 4 months. Then, six-month-old diet-induced obese animals were allocated to sedentarism (DIO) or to a long-term treadmill training (DIOEX) up to 18 months of age. In exercised mice, iWAT depot revealed more adaptability, with an increase in the expression of fatty acid oxidation genes (Cpt1a, Acox1), and an amelioration of the inflammatory status, with a favorable modulation of pro/antiinflammatory genes and lower macrophage infiltration. Additionally, iWAT of trained animals showed an increment in the expression of mitochondrial biogenesis (Pgc1a, Tfam, Nrf1), thermogenesis (Ucp1), and beige adipocytes genes (Cd137, Tbx1). In contrast, iBAT of aged obese mice was less responsive to exercise. Indeed, although an increase in functional brown adipocytes genes and proteins (Pgc1a, Prdm16 and UCP1) was observed, few changes were found on inflammation-related and fatty acid metabolism genes. The remodeling of iWAT and iBAT depots occurred along with an improvement in the HOMA index for insulin resistance and in glucose tolerance. In conclusion, long-term exercise effectively prevented the loss of iWAT and iBAT thermogenic properties during aging and obesity. In iWAT, the long-term exercise program also reduced the inflammatory status and stimulated a fat-oxidative gene profile. These exercise-induced adipose tissue adaptations could contribute to the beneficial effects on glucose homeostasis in aged obese mice.

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“…So far, three beta isoforms of AR have been reported (β1–β3). Although β3-AR predominates in white adipose tissue [ 60 ], studies have confirmed that gene polymorphisms of all three isoforms could affect the fat catabolism induced by aerobic exercise [ 61 – 63 ], although the relationship between HIIT and different isoforms had been unclear. To determine whether the VAT adaptation to HIIT is only related to β3-AR or also to other isoforms, we blocked this receptor with SR59230a, a selective antagonist, concomitantly with 10 μM ISO stimulation.…”

Section: Discussionmentioning

confidence: 99%

Comparison of visceral fat lipolysis adaptation to high-intensity interval training in obesity-prone and obesity-resistant rats

Liu

Cheng

et al. 2022

Diabetol Metab Syndr

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Background/objectives Visceral obesity is one of the key features of metabolic syndrome. High-intensity interval training (HIIT) could effectively reduce visceral fat, but its effects show strong heterogeneity in populations with different degrees of obesity. The mechanism may be related to the differential adaptation to training between obesity phenotypes, namely obesity prone (OP) and obesity resistant (OR). The aim of the present study was to compare adaptive changes of visceral adipose lipolysis adaptation to HIIT between OP and OR animals and further explore the upstream pathway. Methods OP and OR Sprague Dawley rats were established after feeding a high-fat diet for 6 weeks; they were then divided into HIIT (H-OP and H-OR) and control (C-OP and C-OR) groups. After 12 weeks of HIIT or a sedentary lifestyle, animals were fasted for 12 h and then sacrificed for histology as well as gene and protein analysis. Visceral adipocytes were isolated without fasting for catecholamine stimulation and β3-adrenergic receptor (β3-AR) blockade in vitro to evaluate the role of upstream pathways. Results After training, there were no differences in weight loss or food intake between OP and OR rats (P > 0.05). However, the visceral fat mass, adipocyte volume, serum triglycerides and liver lipids of OP rats decreased by more than those of OR rats (P < 0.05). Meanwhile, the cell lipolytic capacity and the increase in the expression of β3-AR were higher in the OP compared with OR groups (P < 0.05). Although training did not increase sympathetic nervous system activity (P > 0.05), the cell sensitivity to catecholamine increased significantly in the OP compared with OR groups (P < 0.05). Following blocking β3-AR, the increased sensitivity disappeared. Conclusion With HIIT, OP rats lost more visceral fat than OR rats, which was related to stronger adaptive changes in lipolysis. Increased β3-AR expression mediated this adaptation.

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Effects of aerobic exercise on the inflammatory cytokine profile and expression of lipolytic and thermogenic genes in β1-AR−/− mice adipose tissue

Cited by 9 publications

References 39 publications

The effect of aerobic exercise training on gene expression of beta3-adrenergic receptor and beta-arrestin2 in inguinal white adipose tissue of mice fed with a high fat diet

The effect of aerobic exercise training on gene expression of beta3-adrenergic receptor and beta-arrestin2 in inguinal white adipose tissue of mice fed with a high fat diet

Differential remodeling of subcutaneous white and interscapular brown adipose tissue by long-term exercise training in aged obese female mice

Comparison of visceral fat lipolysis adaptation to high-intensity interval training in obesity-prone and obesity-resistant rats

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