Effects of acute and chronic arecoline in adult zebrafish: Anxiolytic-like activity, elevated brain monoamines and the potential role of microglia

Serikuly, Nazar; Alpyshov, Erik T.; Wang, Dong Mei; Wang, Jing Tao; Long, Yang; Hu, Guo Jun; Dong, Yan; Demin, Konstantin A.; Колесникова, Т. А.; Galstyan, David S.; Amstislavskaya, Tamara G.; Babashev, Abdrazak M.; Mor, Mikael S.; Efimova, Evgeniya V.; Gainetdinov, Raul R.; Strekalova, Tatyana; Abreu, Murilo S. de; Song, Cai; Kalueff, Allan V.

doi:10.1016/j.pnpbp.2020.109977

Cited by 45 publications

(47 citation statements)

References 60 publications

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“…All fish were from the same population, and were randomly divided into experimental groups using an online random number generator. The strain selection for the present study was based on population validity considerations and their relevance for the present study 77 . Specifically, while genetically controlled inbred zebrafish strains may offer reproducible and more reliable systems for neurogenetics research, modeling CNS disorders (such as in the present study) involves mimicking ‘real’ human maladies that affect genetically heterogenous clinical populations.…”

Section: Methodsmentioning

confidence: 99%

Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model

Demin

Колесникова

Galstyan

et al. 2021

Sci Rep

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Long-term recurrent stress is a common cause of neuropsychiatric disorders. Animal models are widely used to study the pathogenesis of stress-related psychiatric disorders. The zebrafish (Danio rerio) is emerging as a powerful tool to study chronic stress and its mechanisms. Here, we developed a prolonged 11-week chronic unpredictable stress (PCUS) model in zebrafish to more fully mimic chronic stress in human populations. We also examined behavioral and neurochemical alterations in zebrafish, and attempted to modulate these states by 3-week treatment with an antidepressant fluoxetine, a neuroprotective omega-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA), a pro-inflammatory endotoxin lipopolysaccharide (LPS), and their combinations. Overall, PCUS induced severe anxiety and elevated norepinephrine levels, whereas fluoxetine (alone or combined with other agents) corrected most of these behavioral deficits. While EPA and LPS alone had little effects on the zebrafish PCUS-induced anxiety behavior, both fluoxetine (alone or in combination) and EPA restored norepinephrine levels, whereas LPS + EPA increased dopamine levels. As these data support the validity of PCUS as an effective tool to study stress-related pathologies in zebrafish, further research is needed into the ability of various conventional and novel treatments to modulate behavioral and neurochemical biomarkers of chronic stress in this model organism.

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Section: Methodsmentioning

confidence: 99%

Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model

Demin

Колесникова

Galstyan

et al. 2021

Sci Rep

Self Cite

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“…Qualitative real-time polymerase chain reaction (qRT-PCR). To reconfirm the validity of our RNAseq data, we also conducted a small qualitative real-time PCR study of selected genes, quantifying wholebrain expression of four 'reference' genes (isg15, saga, otx5 and tpm4b) and one housekeeping gene (b-act) using the established qrt-PCR protocol 48,119,122,123 , with minor modifications. The genes were chosen based on their hubness in core PPI groups and on different expression patterns between groups (isg15-differentially expressed in both stress and fluoxetine vs. control, saga-in stress vs. fluoxetine, otx5-stress vs. both control and fluoxetine and tpm4b-in stress vs. control).…”

Section: Rna-sequencingmentioning

confidence: 97%

Understanding complex dynamics of behavioral, neurochemical and transcriptomic changes induced by prolonged chronic unpredictable stress in zebrafish

Demin

Lakstygal

Krotova

et al. 2020

Sci Rep

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Stress-related neuropsychiatric disorders are widespread, debilitating and often treatment-resistant illnesses that represent an urgent unmet biomedical problem. Animal models of these disorders are widely used to study stress pathogenesis. A more recent and historically less utilized model organism, the zebrafish (Danio rerio), is a valuable tool in stress neuroscience research. Utilizing the 5-week chronic unpredictable stress (CUS) model, here we examined brain transcriptomic profiles and complex dynamic behavioral stress responses, as well as neurochemical alterations in adult zebrafish and their correction by chronic antidepressant, fluoxetine, treatment. Overall, CUS induced complex neurochemical and behavioral alterations in zebrafish, including stable anxiety-like behaviors and serotonin metabolism deficits. Chronic fluoxetine (0.1 mg/L for 11 days) rescued most of the observed behavioral and neurochemical responses. Finally, whole-genome brain transcriptomic analyses revealed altered expression of various CNS genes (partially rescued by chronic fluoxetine), including inflammation-, ubiquitin- and arrestin-related genes. Collectively, this supports zebrafish as a valuable translational tool to study stress-related pathogenesis, whose anxiety and serotonergic deficits parallel rodent and clinical studies, and genomic analyses implicate neuroinflammation, structural neuronal remodeling and arrestin/ubiquitin pathways in both stress pathogenesis and its potential therapy.

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“…Arecoline is a pyridine-type alkaloid which can quickly cross the blood-brain barrier and that depends on its chemical structure [ 6 ], which lacks quaternary ammonium ions compared with methacholine and has higher lipophilicity, so it can cross the cell membrane into the body better. In each period of betel nut from young fruit to mature and then to processing and placement, the content of arecoline changed accordingly.…”

Section: Chemical Compositionmentioning

confidence: 99%

Chemical Composition, Pharmacological, and Toxicological Effects of Betel Nut

Chen

Deng

2021

Evidence-Based Complementary and Alternative Medicine

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Betel nut, the fruit of Areca catechu L, has a long medical history in Southeast Asia. It is native to Malaysia and is cultivated and processed extensively in subtropical regions, such as South China and India. Betel nut almost appears as a “snack” in various occasions in most parts of China. Clinically, betel nut can play a certain pharmacology role and was used in malaria, ascariasis, arthritis, enterozoic abdominalgia, stagnation of food, diarrhea, edema, and beriberi. The nervous excitement of betel nut chewing has made it gradually become popular. However, chewing betel nut can induce oral submucosal fibrosis (OSF) and oral cancer (OC). At the same time, long-term chewing of betel nut also causes inhaled asthma, sperm reducing, betel quid dependence (BQD), and uterine and esophageal cancers. The main components of processed betel nut are the goal of this review. This study will mainly start from the pharmacological activity and toxicology study of betel nut in recent years, aiming to seek its advantages and disadvantages. In the meantime, this study will analyze and emphasize that betel nut and arecoline are the high-risk factors for oral cancer, which should arouse attention and vigilance of the public.

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Effects of acute and chronic arecoline in adult zebrafish: Anxiolytic-like activity, elevated brain monoamines and the potential role of microglia

Cited by 45 publications

References 60 publications

Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model

Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model

Understanding complex dynamics of behavioral, neurochemical and transcriptomic changes induced by prolonged chronic unpredictable stress in zebrafish

Chemical Composition, Pharmacological, and Toxicological Effects of Betel Nut

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