Effects of abscisic acid and high concentrations of PEG on Hevea brasiliensis somatic embryos development

Linossier, Laurent; Veisseire, Philippe; Cailloux, Françoise; Coudret, A.

doi:10.1016/s0168-9452(97)04597-4

Cited by 67 publications

(32 citation statements)

References 26 publications

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“…The highest concentration of endogenous ABA (17.45 nmol g -1 DW) was recorded after direct 1-week ABA treatment of torpedo embryos of tulip. Similar results have been obtained in somatic embryos of Hevea brasiliensis, while an addition of 10 -5 M of ABA increased its endogenous level from 10 nmol/g DW (control) to above 150 nmol/g DW (Linossier et al 1997). In our experiment, fluridone treatment of the tulip somatic embryos led to a nearly 50% decrease in ABA content.…”

Section: Discussionsupporting

confidence: 85%

“…By controlling gene expression connected with the vegetative development of plants (Seo and Koshiba 2002), ABA stimulates somatic embryo development and quality, which has been observed in many plants, e.g. Asparagus officinalis (Li and Wolyn 1996), Quercus suber (Garcia-Martin et al 2005), Quercus ilex (Mauri and Manzanera 2004), Picea abies (Vágner et al 1998), Hevea brasiliensis (Linossier et al 1997). Fluridone was applied to unambiguously verify the influence of ABA (Gamble andMullet 1986, Kępczyńska andZielińska 2006).…”

Section: Discussionmentioning

confidence: 99%

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Endogenous ABA content in relation to maturation of somatic embryos in Tulipa (L.) ‘Apeldoorn’ cultures

2016

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The aim of the present study was to estimate the endogenous abscisic acid (ABA) content in tulip 'Apel-doorn' torpedo and mature somatic embryos. Moreover, the effect of exogenous ABA and/or its inhibitor fluridone on somatic embryo maturation and conversion into plantlets was investigated. Torpedo-stage somatic embryos were subcultured on media containing 5 lM of picloram and 1 lM of 6-benzyl-aminopurine (BAP)-control, and combinations of ABA (0 or 10 lM) and/or fluridone (0 or 30 lM) for 1 week. Then, the torpedo embryos were transferred to a maturation medium containing 0.25 lM of a-naphthaleneacetic acid (NAA) and 2.5 lM of BAP, without ABA and fluridone treatment, and cultivated under darkness or light for ten weeks. Endogenous ABA content (first time measured in tulip somatic embryos) was evaluated by ELISA test. The obtained results revealed that the highest level of endogenous ABA, at 17.45 nmol g-1 dry weight (DW), was recorded in torpedo-stage of tulip embryo development, only after 1 week of ABA treatment, and was nearly 10 times higher in comparison with the control. Simultaneous addition of ABA and fluridone to the medium resulted in the lowering of the ABA concentration to 9.58 nmol g-1 DW. During ten weeks of maturation of the embryos, the endogenous ABA content in mature tissue of tulip somatic embryo considerably decreased to an amount 0.87-1.33 nmol g-1 DW (irrespective of ABA and fluridone treatment) and did not differ significantly from control (0.59 nmol g-1 DW). Exogenous ABA and fluridone significantly decreased the growth value of fresh weight (FW) of the tulip torpedo-shaped and mature embryos under light conditions. Percentage of the DW of the torpedo embryos treated with exogenous ABA was significantly higher (15.43-17.02) in comparison with the control (10.87). Three to three and a half times more malformed mature embryos were noted under light conditions than in darkness, irrespective of ABA and fluridone treatment. The highest percentage of mature embryos forming shoots (conversion) was observed under light conditions in the control and after fluridone treatment (26 and 20%, respectively).

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Endogenous ABA content in relation to maturation of somatic embryos in Tulipa (L.) ‘Apeldoorn’ cultures

2016

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“…PEG molecules are too large to move through the cell wall and do not cause plasmolysis. Non-plasmolyzing osmotic are more effective in promoting somatic embryo maturation (Linossier et al, 1997;Walker and Parrott, 2001). In our findings conversion of guava somatic embryos into plantlets achieve successfully on 1/2 MS medium fortified with BAP (0.…”

Section: Effect Of Carrier Substrate In Acclimatization Of Plantletsmentioning

confidence: 99%

Recurrent Somatic Embryogenesis and Plantlet Regeneration in Psidium guajava L.

Bajpai

Kalim

Chandra

et al. 2016

Braz. arch. biol. technol.

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“…Exogenous ABA application has also been used to enhance desiccation tolerance in somatic embryos of a diverse range of species including white spruce (Attree et al, 1991), alfalfa (Lecouteux et al, 1993), Havea brasiliensis (Linossier et al, 1997), cassava (Groll et al, 2002) and tea (Mondal et al, 2002) Furthermore, structural abnormality of somatic embryos is linked to low conversion into plant (Rodriguez and Wetzstein, 1994;Soh et al, 1996). It has been reported that 2,4 -D and cytokinins increase abnormal somatic embryos in Aralia cordata (Lee and Soh, 1994) but ABA enhances development of normal embryos in caraway, and carrot (Kamada and Harada, 1981).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Abscisic Acid in the Conversion of Cocoa Somatic Embryos into Plantlets

Quainoo¹

2012

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The effects of abscisic acid (ABA) (0, 10, 30 and 50 μMl -1 ) in embryo development (ED) medium on the conversion of primary and secondary somatic embryos of cocoa at different developmental stages (globular, heart and torpedor) were evaluated in order to overcome the low rate of conversion into plantlets. Genotype COCA 3370-5 was more efficient than genotype AMAZ 3-2 in terms of embryogenesis and response to ABA treatments with secondary embryogenesis being superior over primary embryogenesis. Torpedor stage embryos were more efficient than globular and heart stage embryos in converting into plantlets. The most effective treatments for embryo conversion were 0 and 10 µMl -1 ABA at 2 weeks (wk). ABA concentrations at 0 and 10 µMl -1 were more effective in converting primary and secondary somatic embryos into plantlets than the 30 and 50 µMl -1 . The effects of ABA treatments on conversion of secondary somatic embryos into plantlets were superior to primary embryos. Time of ABA treatment in ED medium influenced the conversion of the somatic embryos. ABA treatments at 0 and 10 µMl -1 recorded decreasing embryo conversion rates from two to six wk, the other treatments recorded higher conversion rates at four wk followed by two and six wk respectively.

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Effects of abscisic acid and high concentrations of PEG on Hevea brasiliensis somatic embryos development

Cited by 67 publications

References 26 publications

Endogenous ABA content in relation to maturation of somatic embryos in Tulipa (L.) ‘Apeldoorn’ cultures

Endogenous ABA content in relation to maturation of somatic embryos in Tulipa (L.) ‘Apeldoorn’ cultures

Recurrent Somatic Embryogenesis and Plantlet Regeneration in Psidium guajava L.

The Effect of Abscisic Acid in the Conversion of Cocoa Somatic Embryos into Plantlets

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