Effects of a twin strain of Saccharomyces cerevisiae live cells on mixed ruminal microorganism fermentation in vitro1

Lila, Zeenat Ara; Mohammed, Nazimuddin; Yasui, Takashi; Kurokawa, Yoshika; Kanda, Shuhei; Itabashi, Hideyuki

doi:10.2527/2004.8261847x

Cited by 110 publications

(119 citation statements)

References 29 publications

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“…In the present study, the rumen ammonia concentration was not affected by the yeast addition, as reported by others (Lila et al, 2004;Mwenya et al, 2005), although slight decreases in the ammonia concentration by LYC and YC for the L-NDF diet were observed. Decreased ammonia concentrations in the rumen caused by Saccharomyces cerevisiae were reported by Abd-El-Ghani (2004) and may indicate lower protein decomposition as well as faster flow of undegraded protein to the duodenum.…”

Section: Resultssupporting

confidence: 88%

“…This effect was not observed in the present study. Lila et al (2004) found in their in vitro study that yeast increased total VFAs and elevated levels of propionate during batch culture incubations, which was confirmed in the case of the LYC additive for the L-NDF diet. Nonetheless, in the experiments conducted by Newbold et al (1995), LYC did not affect VFA concentrations.…”

Section: Resultsmentioning

confidence: 74%

“…Yeast cultures can stimulate utilization of hydrogen by acetogenic bacteria, lactate uptake by Selenomonas ruminatium, and enhance the growth of cellulolytic bacteria (Martin and Nisbet, 1992;Chaucheyras et al, 1995). Live yeast cultures increased the proportion of propionate, decreased the lactate concentration, and increased the numbers of total viable bacteria and cellulolytic bacteria (Newbold et al, 1990;Mutsvangwa et al, 1992;Lila et al, 2004). Corona et al (1999) emphasised considerable inconsistencies between the quoted research results.…”

Section: Introductionmentioning

confidence: 99%

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The effect of <i>Saccharomyces cerevisiae</i> on ruminal fermentation in sheep fed high- or low-NDF rations

Grochowska¹,

Nowak²,

Mikuła³

et al. 2012

J. Anim. Feed Sci.

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The aim of the study was to determine the effect of live yeast culture (LYC) and dehydrated yeast culture (YC) containing Saccharomyces cerevisiae on fermentation parameters and degradability in the rumen of sheep fed a high-(H-NDF; 56% DM) or low-(L-NDF; 35% DM) NDF diet. Three Polish Merino sheep fitted with ruminal cannulas were used. The effective degradability of maize grain and wheat straw was measured by an in situ procedure. Ruminal pH, ammonia, total volatile fatty acid concentrations, and molar percentages of acetate, propionate and butyrate were not modified by the addition of LYC or YC, irrespective of the applied diet. Compared with control (CON) and LYC supplementation of the L-NDF diet significantly (P<0.05) increased the caproate concentration, but in the H-NDF diet, it increased the lactate concentration (P<0.05). In situ rumen NDF degradability of maize grain and wheat straw was not affected by the treatments. When the sheep were fed the H-NDF diet, however, degradability of dry matter maize grain was significantly higher (P<0.05) than in the CON group. The results of this study did not confirm the hypothesis that NDF in the diet is the main reason for the considerable inconsistencies in the results of experiments with Saccharomyces cerevisiae products.

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Section: Resultssupporting

confidence: 88%

Section: Resultsmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

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The effect of <i>Saccharomyces cerevisiae</i> on ruminal fermentation in sheep fed high- or low-NDF rations

Grochowska¹,

Nowak²,

Mikuła³

et al. 2012

J. Anim. Feed Sci.

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show abstract

“…Similarly, Lila et al (2004) observed no effect of a twin-strain of Saccharomyces cerevisiae live cells (0.33 to 1.32 g/l) on pH. In contrast, Bach et al (2007) demonstrated that yeast supplementation (Saccharomyces cerevisiae Strain CNCM I-1077) to dairy cattle (5 g/day) contributes to the rumen environment by enhancing the ruminal pH.…”

Section: Discussionmentioning

confidence: 96%

Effects of hydrolysed yeasts on ruminal fermentation in the rumen simulation technique (Rusitec)

Oeztuerk¹,

Emre²,

Breves³

2016

Vet. Med.

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ABSTRACT:The objective of the present study was to investigate the effects of three different hydrolysed yeast products derived from Saccharomyces cerevisiae [hydrolysed whole yeast (HWY), less hydrolysed whole yeast (LHWY), and yeast cell wall (YCW)] on microbial fermentation characteristics using the rumen simulation technique (Rusitec) with three consecutive experiments. The Rusitec system consisted of six fermentation vessels. Each vessel received 5 g chopped meadow hay and 4 g concentrate (as-fed basis) daily for up to 22 days. Yeast products were added to the fermentation vessels at a concentration of 0.25 or 0.75 g/day. In most cases, ruminal microbial activity was stimulated by HWY and YCW, particularly at the 0.75 g/day level. HWY resulted in a decrease (P < 0.05) in ruminal pH and an increase (P < 0.05) in total short-chain fatty acid (SCFA), acetate, propionate and methane productions, and an increase in NH 3 -N concentration when compared with the control values. Ruminal pH was not altered, but total SCFA, acetate, propionate, butyrate, and methane productions as well as NH 3 -N concentration increased (P < 0.05) in response to YCW treatment. Digestibility of organic matter was not significantly affected by either HWY or YCW. The effects of LHWY on ruminal fermentation characteristics were negligible. These results indicate that degree of hydrolysation (low or high) and composition of yeasts (whole cell or cell wall) have remarkable effects on ruminal microbial activity in the Rusitec system.

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“…After sampling, the bottle was immediately and anaerobically warmed in a portable incubator (39 ± 1°C). Ruminal fluid was transferred to the laboratory and then filtered through four layers of surgical gauze into a polypropylene beaker under flushing with O 2 -free CO 2 CO 3 , and 600 mg cysteine hydrochloride) in accordance with the method of Lila et al [14]. The ruminal fluid (10 ml) and buffer (20 ml) were immediately mixed in a 50 ml polypropylene test tube containing a substrate of micronised wood powder.…”

Section: In Vitro Experimentsmentioning

confidence: 99%

In vitro and in vivo ruminal fermentation of micronised wood powder for volatile fatty acid production in beef cattle

Hamano¹,

Takahashi²,

Agematu³

2017

Anim Husb Dairy Vet Sci

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The aim of the present study was to evaluate in vitro and in vivo ruminal fermentation of micronised wood powder in beef cattle. Micronised wood powder was prepared by pulverizing sapwood of Japanese cedar to a mean particle size of 52 µm. Ruminal fluid from four beef cows was anaerobically incubated with different amounts of micronised wood powder (50, 100, 200, or 400 mg per 30 ml rumen fluid) for 24 hours at 39°C. In the in vivo experiment, four cows were fed micronised wood powder for four weeks in a crossover design. During the initial feeding period of seven days, micronised wood powder was gradually increased to the equivalent to 0.3% of body weight, and then animals were fed micronised wood powder for three weeks at this level. In batch fermentation, total concentrations of volatile fatty acid were increased by micronised wood powder supplementation except at 50 mg. This response was mainly attributable to increased concentrations of the major volatile fatty acids of acetate, propionate, and butyrate. However, volatile fatty acid production from micronised wood powder by in vitro ruminal fermentation was low and limited, even when the supplemented levels were increased to 400 mg. The ruminal fermentability of micronised wood powder was shown by the lack of change in the volatile fatty acid profile during in vivo ruminal fermentation. Therefore, the present study found that ruminal microorganisms enable fermentation and digestion of lignocelluloses in micronised wood powder directly for volatile fatty acid production. However, further consideration of micronised wood powder, such as improvement in particle size or lignin, is needed to accelerate the fermentation kinetics of micronised wood powder in the rumen.

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Effects of a twin strain of Saccharomyces cerevisiae live cells on mixed ruminal microorganism fermentation in vitro1

Cited by 110 publications

References 29 publications

The effect of <i>Saccharomyces cerevisiae</i> on ruminal fermentation in sheep fed high- or low-NDF rations

The effect of <i>Saccharomyces cerevisiae</i> on ruminal fermentation in sheep fed high- or low-NDF rations

Effects of hydrolysed yeasts on ruminal fermentation in the rumen simulation technique (Rusitec)

In vitro and in vivo ruminal fermentation of micronised wood powder for volatile fatty acid production in beef cattle

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