Effects of a new generation of fish protein hydrolysate on
                    performance, intestinal microbiology, and immunity of broiler
                    chickens

Khattab, A.; Peters, Sunday O.; Adenaike, A. S.; Sallam, A. A.; Atya, Mahasan; Ahmed, Heba A.

doi:10.5187/jast.2022.e99

Cited by 3 publications

(1 citation statement)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These species are notorious pathogens implicated in causing substantial foodborne illnesses in humans, despite their asymptomatic manifestation in poultry hosts [ 37 , 47 , 48 ]. Concurrently, this intervention fosters the proliferation of advantageous microbial communities, exhibiting a composition distinctively varied from those observed in broilers, prominently including genera such as Oscioospira , Lactobacillus , and Coprococcus [ 37 , 49 , 50 ]. Among these, Coprococcus is particularly noteworthy for its commensal attributes, contributing to the enhancement of gut health through its role in fermentation processes and the synthesis of SCFAs [ 51 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

Dietary L-Methionine modulates the gut microbiota and improves the expression of tight junctions in an in vitro model of the chicken gastrointestinal tract

Kwak,

Kang,

Eor

et al. 2024

anim microbiome

View full text Add to dashboard Cite

Background The poultry industry encounters a number of factors that affect growth performance and productivity; nutrition is essential for sustaining physiological status and protecting against stressors such as heat, density, and disease. The addition of vitamins, minerals, and amino acids to the diet can help restore productivity and support the body’s defense mechanisms against stress. Methionine (Met) is indispensable for poultry’s energy metabolism, physiology, performance, and feed utilization capacity. Through this study, we aimed to examine the physiological effects of methionine supplementation on poultry as well as alterations of intestinal microbiome. Methods We utilized the DL- and L- form of methionine on Caenorhabditis elegans and the FIMM (Fermentor for intestine microbiota model) in-vitro digesting system. A genomic-analysis of the transcriptome confirmed that methionine supplementation can modulate growth-related physiological metabolic pathways and immune responses in the host poultry. The C. elegans model was used to assess the general health benefits of a methionine supplement for the host. Results Regardless of the type or concentration of methionine, supplementation with methionine significantly increased the lifespan of C. elegans. Feed grade L-Methionine 95%, exhibited the highest lifespan performance in C. elegans. Methionine supplementation increased the expression of tight junction genes in the primary intestinal cells of both broiler and laying hens, which is directly related to immunity. Feed grade L-Methionine 95% performed similarly or even better than DL-Methionine or L-Methionine treatments with upper doses in terms of enhancing intestinal integrity. In vitro microbial cultures of healthy broilers and laying hens fed methionine revealed changes in intestinal microflora, including increased Clostridium, Bacteroides, and Oscillospira compositions. When laying hens were given feed grade L-Methionine 95% and 100%, pathogenic Campylobacter at the genus level was decreased, while commensal bacteria were increased. Conclusions Supplementation of feed grade L-Methionine, particularly L-Methionine 95%, was more beneficial to the host poultry than supplementing other source of methionine for maintaining intestinal integrity and healthy microbiome.

show abstract

Section: Discussionmentioning

confidence: 99%

Dietary L-Methionine modulates the gut microbiota and improves the expression of tight junctions in an in vitro model of the chicken gastrointestinal tract

Kwak,

Kang,

Eor

et al. 2024

anim microbiome

View full text Add to dashboard Cite

show abstract

Cottonseed meal protein hydrolysate influences growth performance, carcass characteristics, serum biochemical indices, and intestinal morphology in yellow‐feather broilers

Zhang,

Nan,

Zhang

et al. 2024

Animal Physiology Nutrition

View full text Add to dashboard Cite

This study investigated the effects of cottonseed meal protein hydrolysate (CPH) on the growth performance, carcass characteristics, serum biochemical indices, intestinal morphology, and enzyme activities of yellow‐feather broilers. We randomly divided 240 chicks into four groups, each with six replicates: a basal diet with 0% (CON), 1% (LCPH), 3% (MCPH), or 5% (HCPH) CPH. The trail spanned 63 days and included three phases: Days 1–21, 22–42, and 43–63. Increased average daily gain (ADG) and decreased ratio of feed to gain (F/G) with LCPH were observed in 21‐day‐old broilers (P < 0.05). MCPH led to higher ADG and average daily feed intake (ADFI) in 42‐day‐old broilers (P < 0.05). Additionally, CPH supplementation resulted in increased dressing percentage, percentage of half‐eviscerated yield, percentage of eviscerated yield, breast muscle rate, and leg muscle rate were observed (P < 0.05) with diet. The serum levels of total protein (TP), high‐density lipoprotein cholesterol (HDL‐C), calcium (Ca), and phosphorus (P) were enhanced, and blood urea nitrogen (BUN) and triglyceride (TG) levels decreased with diet and CPH (P < 0.05). CPH increased the length of the jejunum and ileum and the weight of the duodenum, jejunum, and ileum in 21‐day‐old broilers (P < 0.05). Alterations in the duodenal villus structure in broilers occurred on Days 21 and 42, and the CPH groups performed better; however, a similar change occurred in the jejunum on Days 42 and 63 (P < 0.05). MCPH and HCPH enhanced trypsin activity in the duodenum of 21‐day‐old and 63‐day‐old broilers (p < 0.05). Chymotrypsin activity increased (P > 0.05) in the duodenum of 63‐day‐old broilers fed MCPH. Lipase activity increased (P < 0.05) in the jejuna of 21‐day‐old broilers treated with HCPH. CPH increased trypsin activity in the ilea of 21‐day‐old broilers (P < 0.05). These results showed that CPH influenced the growth performance, carcass characteristics, serum biochemical indices, and intestinal morphology of yellow‐feather broilers, which are related to growth stage. The recommended CPH level in broilers is 1% before 21 days of age and 3% after 21 days of age.

show abstract

Effects of cottonseed meal protein hydrolysate on intestinal microbiota of yellow-feather broilers

Zhang,

Wang,

Niu

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

We evaluated the effects of cottonseed meal protein hydrolysate (CPH) on the intestinal microbiota of yellow-feather broilers. We randomly divided 240 chicks into four groups with six replicates: basal diet with 0% (CON), 1% (LCPH), 3% (MCPH), or 5% (HCPH) CPH. The test lasted 63 days and included days 1–21, 22–42, and 43–63 phases. The ACE, Chao1, and Shannon indices in the MCPH and HCPH groups of 42-day-old broilers were higher than those in the CON group (p < 0.05), indicating that the cecum microbial diversity and richness were higher in these groups. Firmicutes and Bacteroidetes were the dominant phyla; however, the main genera varied during the different periods. The abundance of Lactobacillus in CPH treatment groups of 21-day-old broilers was high (p < 0.05); in the 42-day-old broilers, the abundances of Barnesiella, Clostridia_vadinBB60_group, and Parasutterella in the LCPH group, Desulfovibrio, Lactobacillus, Clostridia_vadinBB60_group, and Butyricicoccus in the MCPH group, and Megamonas and Streptococcus in the HCPH group increased; in the 63-day-old broilers, the abundance of Clostridia_UCG-014 and Synergistes in the LCPH and HCPH group, respectively, increased (p < 0.05), and that of Alistipes in the LCPH and MCPH groups decreased (p < 0.05). And changes in the abundance of probiotics were beneficial to improve the intestinal morphology and growth performance. In addition, the LCPH treatment increased the complexity of the microbial network, while the MCPH treatment had the same effect in 42-day-old broilers. Thus, CPH increased the relative abundance of beneficial intestinal microbiota and enhanced the richness and diversity of the bacterial microbiota in broilers aged <42 days; this effect was weakened after 42 days.

show abstract

Effects of a new generation of fish protein hydrolysate on performance, intestinal microbiology, and immunity of broiler chickens

Abstract: Effects of a new generation of fish protein hydrolysate on performance, intestinal microbiology, and immunity of broiler chickens

Cited by 3 publications

References 37 publications

Dietary L-Methionine modulates the gut microbiota and improves the expression of tight junctions in an in vitro model of the chicken gastrointestinal tract

Dietary L-Methionine modulates the gut microbiota and improves the expression of tight junctions in an in vitro model of the chicken gastrointestinal tract

Cottonseed meal protein hydrolysate influences growth performance, carcass characteristics, serum biochemical indices, and intestinal morphology in yellow‐feather broilers

Effects of cottonseed meal protein hydrolysate on intestinal microbiota of yellow-feather broilers

Contact Info

Product

Resources

About