Effects of a Grapevine Shoot Extract Containing Resveratrol and Resveratrol Oligomers on Intestinal Adenoma Development in Mice: In Vitro and In Vivo Studies

Empl, Michael T.; Cai, Hong; Wang, Shan; Junginger, Johannes; Kostka, Tina; Hewicker‐Trautwein, M.; Brown, Karen; Gescher, Andreas J.; Steinberg, Pablo

doi:10.1002/mnfr.201700450

Cited by 10 publications

(6 citation statements)

References 41 publications

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“…Resveratrol, a naturally occurring antibiotic plant, has been shown to have potent anti-tumor effect through suppressing proliferation and inducing apoptosis in multiple cancers. 17 , 18 In this study, we also found that resveratrol, as an SIRT1 activator, can also induce protective autophagy which may inhibit the anti-tumor effect of resveratrol via inhibiting Akt/ mTOR and activating p38-MAPK. Therefore, suppressing protective autophagy enhanced anti-tumor effect of resveratrol, making it an effective reagent against NSCLC.…”

Section: Discussionsupporting

confidence: 55%

Resveratrol, an activator of SIRT1, induces protective autophagy in non-small-cell lung cancer via inhibiting Akt/mTOR and activating p38-MAPK

Wang

Cao

et al. 2018

OTT

127

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BackgroundResveratrol, a natural polyphenolic phytoalexin, has potent anti-tumor activity. Recently, it was found to induce autophagy in cancer cells. However, the effects of resveratrol on autophagy in non-small-cell lung cancer (NSCLC) cells have not yet been clearly elucidated.Materials and methodsA549 and H1299 cells were treated with different concentrations of resveratrol. Cell growth and apoptosis were measured by CCK-8 assay and flow cytometry, respectively. A549 cells were then treated with 200 μM resveratrol or SRT1720. Cell autophagy was detected by western blot and immunofluorescence.ResultsIn this study, we found that resveratrol exerted the anti-tumor effect through inhibiting cell proliferation and promoting cell apoptosis in NSCLC cells dose-dependently. Resveratrol has also increased the relative expression of Beclin1 and LC3 II/I while decreased p62 expression, suggesting that resveratrol induced autophagy in NSCLC cells. In addition, resveratrol increased SIRT1 expression and SIRT1 activator SRT1720-induced autophagy of NSCLC cells. SIRT1 knockdown reduced resveratrol-induced autophagy significantly. These results indicated that resveratrol might induce autophagy through upregulating SIRT1 expression. Moreover, inhibiting autophagy by autophagy inhibitor 3-methyladenine or SIRT1 inhibitor nicotinamide significantly suppressed proliferation while promoted apoptosis compared with the resveratrol 200 μM group, suggesting that resveratrol-induced autophagy might act as a protective mechanism to promote NSCLC cell survival and inhibiting autophagy can enhance the anti-tumor effect of resveratrol. Besides that, resveratrol treatment inhibited Akt/mTOR while p38-MAPK was activated in NSCLC cells in a dose-dependent manner. Activating Akt/ mTOR pathway by IGF-1 or inhibiting p-38-MAPK pathway by doramapimod significantly inhibited cell proliferation while increased cell apoptosis of NSCLC cells compared with the resveratrol 200 μM group.ConclusionTaken together, our findings suggest that resveratrol inhibited proliferation but induced apoptosis and autophagy via inhibiting Akt/mTOR and activating p38-MAPK pathway. Resveratrol-induced autophagy might act as a protective mechanism to promote NSCLC cell survival. Therefore, inhibition of autophagy may enhance the anti-tumor activity of resveratrol in NSCLC.

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Section: Discussionsupporting

confidence: 55%

Resveratrol, an activator of SIRT1, induces protective autophagy in non-small-cell lung cancer via inhibiting Akt/mTOR and activating p38-MAPK

Wang

Cao

et al. 2018

OTT

127

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“…Grapevine shoot extracts were tested by Empl et al [ 138 ] in the prevention of human gastrointestinal cancer. Using an ApcMin mice model, which was subject to an HFD like a human model of adenomatous polyposis, the authors observed that grapevine shoot extracts reduce the number (in males) and volume (in females) of intestinal adenoma.…”

Section: Health Benefitsmentioning

confidence: 99%

The Wine Industry By-Products: Applications for Food Industry and Health Benefits

Ferrer‐Gallego

Silva

2022

Antioxidants

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Each year, 20 million tons of wine by-products are generated, corresponding to 30% of the total quantity of vinified grapes. Wine by-products are a source of healthy bioactive molecules, such as polyphenols and other molecules (pigments, fibers, minerals, etc.). The abundance of bioactive compounds assures a promising future for nutritional foodstuff production. Wine by-products can be used to fortify aromatized waters and infusions, bread, pasta, dairy products, alcohol, sugary beverages, and processed foods. These innovative products are part of the Mediterranean diet and are of great interest to both human and environmental health. Pre-clinical studies show that consumption of food produced with wine by-products or with their extracts attenuates the inflammatory state and increases antioxidant status. As such, wine by-products provide protective effects against the underlying pathophysiological hallmarks of some chronic diseases such as atherosclerosis, diabetes, hypertension, obesity, and cancer. However, the poor bioavailability warrants further investigation on how to optimize the efficacy of wine by-products, and more clinical trials are also needed. The scientific evidence has validated the uses of the dietary nature of wine by-products and has helped to promote their use as a functional food to prevent chronic human diseases.

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“…Two experimental pathways have been adopted by Empl et al [ 119 ] to investigate the possibility of using grapevine shoots extracts as agents in the prevention of human gastrointestinal cancer. In vivo research on ApcMin mice, which were subject to a high-fat diet similar to a human model of adenomatous polyposis, reported that both low and high doses of grapevine shoot extracts have the ability to reduce the number (in males) and volume (in females) of intestinal adenoma.…”

Section: Potential Uses Of Grapevine Waste-derived Products In Biomed...mentioning

confidence: 99%

Grapevine Wastes: A Rich Source of Antioxidants and Other Biologically Active Compounds

et al. 2022

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Wine production is one of the most critical agro-industrial sectors worldwide, generating large amounts of waste with negative environmental impacts, but also with high economic value and several potential applications. From wine shoots to grape pomace or seeds, all of the wastes are rich sources of bioactive compounds with beneficial effects for human health, with these compounds being raw materials for other industries such as the pharmaceutical, cosmetic or food industries. Furthermore, these compounds present health benefits such as being antioxidants, supporting the immune system, anti-tumoral, or preventing cardiovascular and neural diseases. The present work aims to be a critical discussion of the extraction methods used for bioactive compounds from grapevine waste and their beneficial effects on human health.

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Effects of a Grapevine Shoot Extract Containing Resveratrol and Resveratrol Oligomers on Intestinal Adenoma Development in Mice: In Vitro and In Vivo Studies

Abstract: Vineatrol®30 may merit further investigation as a potential dietary gastrointestinal cancer chemopreventive agent in humans.

Cited by 10 publications

References 41 publications

Resveratrol, an activator of SIRT1, induces protective autophagy in non-small-cell lung cancer via inhibiting Akt/mTOR and activating p38-MAPK

Resveratrol, an activator of SIRT1, induces protective autophagy in non-small-cell lung cancer via inhibiting Akt/mTOR and activating p38-MAPK

The Wine Industry By-Products: Applications for Food Industry and Health Benefits

Grapevine Wastes: A Rich Source of Antioxidants and Other Biologically Active Compounds

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