Effects of 7 T static magnetic fields on the expression of biological markers and the formation of bone in rats

Yamaguchi-Sekino, Sachiko; Kira, Tsutomu; Sekino, Masaki; Akahane, Manabu

doi:10.1002/bem.22161

Cited by 8 publications

(4 citation statements)

References 35 publications

(65 reference statements)

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“…In particular, the effect of the magnetic field strength on cells has been a focus of research. As reported, S. Yamaguchi-Sekino, T. Kira, M. Sekino et al found that the differentiation ability of cells was inhibited under the high-intensity magnetic field environment of 7 T [41]. They consider that SMFs may interfere with the opening of ion channels and hinder BMSC osteogenesis.…”

Section: Discussionmentioning

confidence: 87%

Magnesium–Magnetic Field Synergy Enhances Mouse Bone Marrow Mesenchymal Stem Cell Differentiation into Osteoblasts Via the MAGT1 Channel

Wang

Yang

et al. 2022

Journal of Nanomaterials

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Magnesium ion (Mg2+)-based materials are known to exert osteogenic effects that can be enhanced by the bioelectrical properties of magnetic fields. In this study, we examined the effect of a medium-strength static magnetic field (SMF), combined with a Mg2+-containing medium, on the proliferation and osteogenic differentiation of mouse bone marrow mesenchymal stem cells (BMSCs). Mouse BMSCs were divided into a control group, 7.5 mM Mg2+ group, 15 mT SMF group, and 7.5 mM Mg2+ plus 15 mT SMF group. Osteoblast proliferation was measured using a Cell Counting Kit-8 assay, whereas osteogenic differentiation was detected using alkaline phosphatase (ALP) staining and western blot analysis, respectively. The number and size of calcium nodules were determined using Alizarin Red staining. Compared with those in the control group, the ALP activity, calcium nodule formation, and osteogenic protein expression were promoted in other groups. In particular, Mg2+-SMF had a significant effect after 7 days of intervention and more effectively promoted BMSC differentiation and proliferation than either Mg2+ or the SMF alone, suggesting that Mg2+-SMF synergistically contributed to osteogenic differentiation and cell proliferation. To examine their roles in bone differentiation, the Magt1 and Creb1 genes were silenced in BMSCs, and the findings indicated that the synergistic intervention with Mg2+ and magnetic fields might exert osteogenic effects via the MAGT1 channel and CREB1 protein. This study provides an experimental basis for a potential Mg2+-SMF synergistic artificial bone material that could be clinically applied in the treatment of bone defects.

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Section: Discussionmentioning

confidence: 87%

Magnesium–Magnetic Field Synergy Enhances Mouse Bone Marrow Mesenchymal Stem Cell Differentiation into Osteoblasts Via the MAGT1 Channel

Wang

Yang

et al. 2022

Journal of Nanomaterials

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“… 38 While most studies showed positive effects, Yamaguchi-Sekino et al reported that exposure to 7 T SMF for 3 h/day for 6 days had no effect on the expression of genes and proteins of osteogenic markers of MSCs in tricalcium phosphate (TCP)/MSC constructs. 39 …”

Section: Bone Regenerationmentioning

confidence: 99%

Static magnetic fields in regenerative medicine

Xie,

Song,

Guo

et al. 2024

APL Bioengineering

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All organisms on Earth live in the weak but ubiquitous geomagnetic field. Human beings are also exposed to magnetic fields generated by multiple sources, ranging from permanent magnets to magnetic resonance imaging (MRI) in hospitals. It has been shown that different magnetic fields can generate various effects on different tissues and cells. Among them, stem cells appear to be one of the most sensitive cell types to magnetic fields, which are the fundamental units of regenerative therapies. In this review, we focus on the bioeffects of static magnetic fields (SMFs), which are related to regenerative medicine. Most reports in the literature focus on the influence of SMF on bone regeneration, wound healing, and stem cell production. Multiple aspects of the cellular events, including gene expression, cell signaling pathways, reactive oxygen species, inflammation, and cytoskeleton, have been shown to be affected by SMFs. Although no consensus yet, current evidence indicates that moderate and high SMFs could serve as a promising physical tool to promote bone regeneration, wound healing, neural differentiation, and dental regeneration. All in vivo studies of SMFs on bone regeneration and wound healing have shown beneficial effects, which unravel the great potential of SMFs in these aspects. More mechanistic studies, magnetic field parameter optimization, and clinical investigations on human bodies will be imperative for the successful clinical applications of SMFs in regenerative medicine.

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“…In the meantime, magnetic fields have various effects on living organisms ( 5 , 6 , 7 ), but the underlying mechanisms still remain incompletely understood. Similar to other nonliving materials, biological samples may be affected by magnetic torque and/or force in magnetic fields, and one of the most important physical quantities is their magnetic susceptibility, the degree of magnetization in response to an applied magnetic field ( 5 , 8 ).…”

Section: Introductionmentioning

confidence: 99%

Magnetic Susceptibility Difference-Induced Nucleus Positioning in Gradient Ultrahigh Magnetic Field

Tao

Zhang

Han

et al. 2019

Biophysical Journal

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Despite the importance of magnetic properties of biological samples for biomagnetism and related fields, the exact magnetic susceptibilities of most biological samples in their physiological conditions are still unknown. Here we used superconducting quantum interferometer device to detect the magnetic properties of nonfixed, nondehydrated live cell and cellular fractions at a physiological temperature of 37°C (310 K). It is obvious that there are paramagnetic components within human nasopharyngeal carcinoma CNE-2Z cells. More importantly, the magnetic properties of the cytoplasm and nucleus are different. Although within a single cell, the magnetic susceptibility difference between cellular fractions (nucleus and cytoplasm) could only cause ∼41–130 pN forces to the nucleus by gradient ultrahigh magnetic fields of 13.1–23.5 T (92–160 T/m), these forces are enough to cause a relative position shift of the nucleus within the cell. This not only demonstrates the importance of magnetic susceptibility in the biological effects of magnetic field but also illustrates the potential application of high magnetic fields in biomedicine.

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Effects of 7 T static magnetic fields on the expression of biological markers and the formation of bone in rats

Cited by 8 publications

References 35 publications

Magnesium–Magnetic Field Synergy Enhances Mouse Bone Marrow Mesenchymal Stem Cell Differentiation into Osteoblasts Via the MAGT1 Channel

Magnesium–Magnetic Field Synergy Enhances Mouse Bone Marrow Mesenchymal Stem Cell Differentiation into Osteoblasts Via the MAGT1 Channel

Static magnetic fields in regenerative medicine

Magnetic Susceptibility Difference-Induced Nucleus Positioning in Gradient Ultrahigh Magnetic Field

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