Effects of 50Hz sinusoidal electromagnetic fields of different intensities on proliferation, differentiation and mineralization potentials of rat osteoblasts

Zhou, Jian; Ming, Leiguo; Ge, Bao-Feng; Wang, Jiaqi; Zhu, Ran; Wei, Zhe; Ma, Hui‐Ping; Chen, Xian; Chen, Keming

doi:10.1016/j.bone.2011.06.026

Cited by 70 publications

(77 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increase in the intracellular Ca 2C is associated with the differentiation and maturation of the osteoblasts and is reported to increase the osteogenic activity. [38] Our biomechanical test results also indicated a similar improved bone healing by activating osteoblasts when rabbits were exposed to 2100 MHz EMF.…”

Section: Histopathological Analysissupporting

confidence: 66%

“…[36,37] On the contrary, in an in vitro study, it was reported that 50 Hz 1.8 mT and 3.6 mT sinusoidal EMF inhibited the osteoblast proliferation and increased osteoblast differentiation and mineralization. [38] In another in vitro study, it was reported that 50 Hz EMF at 0.8 mT increased the intracellular free calcium (Ca 2C ) level in osteoblasts. [39] Intracellular free calcium has a role as a second messenger in the cell and plays a part in the regulation of many physiological functions.…”

Section: Histopathological Analysismentioning

confidence: 99%

See 1 more Smart Citation

Effect of 2100 MHz mobile phone radiation on healing of mandibular fractures: an experimental study in rabbits

Durğun

Daşdağ

Erbatur

et al. 2015

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Fractures in the maxillofacial region are among the most frequent incidences in patients exposed to trauma. The aim of this study was to evaluate the effect of 2100 MHz electromagnetic radiation on the healing of mandibular fractures in rabbits. The study was carried out on 12 mature male New Zealand rabbits. After producing a left mandibular osteotomy, the fracture was repaired by using a plate screw. The rabbits were divided into two groups À control and experimental. The experimental group was exposed for 3 h daily to a 2100 MHz radiofrequency (RF) for 28 d. Biomechanical, histopathological and radiological tests were performed during the healing of the mandibular bone. According to the biomechanical tests, the energy absorption capacity, toughness and maximum strength of the mandibular bone were increased compared to the control group (6.84 vs. 2.57, 0.135 vs. 0.041 and 22.21 vs. 10.32, respectively). No significant differences between the two groups were found during the radiological assessment. However, the histopathological examination revealed that the fracture healing score was higher in the experimental group when compared to the control group (8.0 vs. 2.75, respectively). In conclusion, the results of this study showed that a 2100 MHz RF radiation positively affected fracture healing.

show abstract

Section: Histopathological Analysissupporting

confidence: 66%

Section: Histopathological Analysismentioning

confidence: 99%

Effect of 2100 MHz mobile phone radiation on healing of mandibular fractures: an experimental study in rabbits

Durğun

Daşdağ

Erbatur

et al. 2015

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

show abstract

“…After washing, calcified nodules were identified under light microscopy and photographed. The number and total area of red nodules were measured by Image-Pro Plus 6.0 software (Zhou et al 2011).…”

Section: Alizarin Red-s Stainingmentioning

confidence: 99%

“…After the cultures were fixed in 3.7 % formaldehyde and 90 % ethanol solution for 1 min, they were stained with a 20 mL Michaelis buffer (pH 8.9, containing 10 mg 1-naphthyl phosphate sodium and 10 mg Fast Blue RR salt) for 15-20 min at 37°C. The number and total area of blue spots were measured by Image-Pro Plus 6.0 software (Zhou et al 2011).…”

Section: Alp Activity Measurement and Histochemical Stainingmentioning

confidence: 99%

Effects of pyrite bioleaching solution of Acidithiobacillus ferrooxidans on viability, differentiation and mineralization potentials of rat osteoblasts

Zhou

Chen²,

Zhi

et al. 2015

Arch. Pharm. Res.

Self Cite

View full text Add to dashboard Cite

Iron pyrite, an important component of traditional Chinese medicine, has a poor solubility, bioavailability, and patient compliance due to a high dose required and associated side effects, all of which have limited its clinical applications and experimental studies on its action mechanisms in improving fracture healing. This study investigated Acidithiobacillus ferrooxidans (A.f)-bioleaching of two kinds of pyrites and examined bioactivities of the derived solutions in viability and osteogenic differentiation in rat calvarial osteoblasts. A.f bioleaching improved element contents (Fe, Mn, Zn, Cu, and Se) in the derived solutions and the solutions concentration-dependently affected osteoblast viability and differentiation. While the solutions had no effects at low concentrations and inhibited the osteoblast alkaline phosphatase (ALP) activity at high concentrations, they improved ALP activity at their optimal concentrations. The improved osteoblast differentiation and osteogenic function at optimal concentrations were also revealed by levels of ALP cytochemical staining, calcium deposition, numbers and areas of mineralized nodules formed, mRNA and protein expression levels of osteogenesis-related genes (osteocalcin, Bmp-2, Runx-2, and IGF-1), and Runx-2 nuclear translocation. Data from this study will be useful in offering new strategies for improving pyrite bioavailability and providing a mechanistic explanation for the beneficial effects of pyrite in improving bone healing.

show abstract

“…Moreover, a recent research suggested that PEMFs activated the TGF-β signaling via Smad2 in differentiated and mineralizing osteoblasts and augmented the expression of osteoblast differentiation marker genes, such as ALP and type I collagen, andexerted its osteogenesis promotional function [3]. The expression of BMPs in osteogenesis was also enhanced by PEMFs according to in vitro and clinical studies [5, 61, 62]. Furthermore, another recent study revealed that PEMFs stimulate osteogenic differentiation and maturation of osteoblasts by primary cilium-mediated upregulated expression of BMPRII, one of the receptors of BMPs, and subsequently activation of BMP–Smad1/5/8 signaling [63].…”

Section: Underlying Signaling Pathwaysmentioning

confidence: 99%

Underlying Signaling Pathways and Therapeutic Applications of Pulsed Electromagnetic Fields in Bone Repair

Yuan

Xin

Jiang

2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Pulsed electromagnetic field (PEMF) stimulation, as a prospective, noninvasive, and safe physical therapy strategy to accelerate bone repair has received tremendous attention in recent decades. Physical PEMF stimulation initiates the signaling cascades, which effectively promote osteogenesis and angiogenesis in an orchestrated spatiotemporal manner and ultimately enhance the self-repair capability of bone tissues. Considerable research progresses have been made in exploring the underlying cellular and subcellular mechanisms of PEMF promotion effect in bone repair. Moreover, the promotion effect has shown strikingly positive benefits in the treatment of various skeletal diseases. However, many preclinical and clinical efficacy evaluation studies are still needed to make PEMFs more effective and extensive in clinical application. In this review, we briefly introduce the basic knowledge of PEMFs on bone repair, systematically elaborate several key signaling pathways involved in PEMFs-induced bone repair, and then discuss the therapeutic applications of PEMFs alone or in combination with other available therapies in bone repair, and evaluate the treatment effect by analyzing and summarizing recent literature.

show abstract

Effects of 50Hz sinusoidal electromagnetic fields of different intensities on proliferation, differentiation and mineralization potentials of rat osteoblasts

Cited by 70 publications

References 32 publications

Effect of 2100 MHz mobile phone radiation on healing of mandibular fractures: an experimental study in rabbits

Effect of 2100 MHz mobile phone radiation on healing of mandibular fractures: an experimental study in rabbits

Effects of pyrite bioleaching solution of Acidithiobacillus ferrooxidans on viability, differentiation and mineralization potentials of rat osteoblasts

Underlying Signaling Pathways and Therapeutic Applications of Pulsed Electromagnetic Fields in Bone Repair

Contact Info

Product

Resources

About