Effects of 17β-estradiol on expression of muscarinic acetylcholine receptor subtypes and estrogen receptor α in rat hippocampus

Cardoso, Camila Castellan; Ricardo, Victor Proença; Frussa‐Filho, Roberto; Porto, Catarina S.; Abdalla, Fernando Maurício Francis

doi:10.1016/j.ejphar.2010.02.032

Cited by 39 publications

(38 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There are several existing reports linking steroids to muscarinic receptor expression in nonreproductive tissues, 17 although the situation in the uterine circulation is not known. Moreover, estrogen-sensitive muscarinic receptor expression and its signal transduction in the uterine arterial endothelium have been linked to multiple vasodilatory pathways, for example, nitric oxide, prostanoids, and endothelium-derived hyperpolarization factor (EDHF).…”

Section: Discussionmentioning

confidence: 99%

Local Versus Systemic Influences on Uterine Vascular Reactivity During Pregnancy in the Single-Horn Gravid Rat

et al. 2011

View full text Add to dashboard Cite

This study was conducted to determine whether gestational changes in maternal uterine artery reactivity are primarily driven by local vs. systemic factors. Rats underwent surgical ligation of one oviduct, thereby restricting implantation and pregnancy to one uterine horn while maintaining a gestational endocrine milieu. Uterine arcuate arteries were isolated and cannulated to evaluate reactivity. Vessels from the implanted horn were significantly more sensitive to phenylephrine and less sensitive to sodium nitroprusside than those from the non-implanted horn; endothelial basal calcium levels were only increased in the implanted horn. Conversely, there were no differences in sensitivity to acetylcholine, or its effects on endothelial cell calcium, although efficacy was greater in vessels from the implanted vs. non-implanted horn. These findings demonstrate that local factors are predominant in inducing changes in vascular smooth muscle function, while endothelial adaptations result from an interplay between local and systemic factors, with distinct effects attributable to each.

show abstract

Section: Discussionmentioning

confidence: 99%

Local Versus Systemic Influences on Uterine Vascular Reactivity During Pregnancy in the Single-Horn Gravid Rat

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Other data also support a relative resistance of females to the effects of pilocarpine on behavior, such as the ability of pilocarpine to stimulate drinking (Fregly, 1980). The mechanism of resistance to muscarinic cholinergic agonists in females may be related to 17β-estradiol, because 17β-estradiol treatment of ovariectomized rats reduces muscarinic receptors in hippocampus (Cardoso et al, 2010). Ovariectomy followed by 17β-estradiol replacement also reduces hippocampal concentrations of [3] inositol phosphate, which is normally increased by muscarinic receptor activation (Pereira et al, 2008).…”

Section: Seizures Epileptogenesis and Epilepsy In Rodentsmentioning

confidence: 99%

Sex differences in the neurobiology of epilepsy: A preclinical perspective

Scharfman

MacLusky

2014

Neurobiology of Disease

124

111

View full text Add to dashboard Cite

When all of the epilepsies are considered, sex differences are not always clear, despite the fact that many sex differences are known in the normal brain. Sex differences in epilepsy in laboratory animals are also unclear, although robust effects of sex on seizures have been reported, and numerous effects of gonadal steroids have been shown throughout the rodent brain. Here we discuss several reasons why sex differences in seizure susceptibility are unclear or are difficult to study. Examples of robust sex differences in laboratory rats, such as the relative resistance of adult female rats to the chemoconvulsant pilocarpine compared to males, are described. We also describe a novel method that has shed light on sex differences in neuropathology, which is a relatively new techniques that will potentially contribute to sex differences research in the future. The assay we highlight uses the neuronal nuclear antigen NeuN to probe sex differences in adult male and female rats and mice. In females, weak NeuN expression defines a sex difference that previous neuropathological studies have not described. We also show that in adult rats, social isolation stress can obscure the normal effects of 17β-estradiol to increase excitability in area CA3 of hippocampus. These data underscore the importance of controlling behavioral stress in studies of seizure susceptibility in rodents and suggest that behavioral stress may be one factor that has led to inconsistencies in outcomes of sex differences research. These and other issues have made it difficult to translate our increasing knowledge about the effects of gonadal hormones on the brain to improved treatment for men and women with epilepsy.

show abstract

“…Estradiol appears to be able alone to support the survival of transplanted cholinergic neurons (Tanaka et al, 1993), can directly modify the expression of muscarinic receptors (Cardoso et al, 2010) and receptor binding (Dohanich et al, 1982), as well as increasing acetylcholinesterase activity in the hypothalamus (Luine and Rhodes, 1983). Estradiol may have nicotinic effects as well, including increasing the density of hypothalamic α-bungarotoxin nicotinic receptors (Morley et al, 1983), and facilitating the response of neurons to the excitatory actions of acetylcholine (Kow and Pfaff, 1985).…”

Section: Estrogen Effects On Cholinergic Systemsmentioning

confidence: 99%

Estrogen–cholinergic interactions: Implications for cognitive aging

Newhouse

Dumas

2015

Hormones and Behavior

View full text Add to dashboard Cite

While many studies in humans have investigated the effects of estrogen and hormone therapy on cognition, potential neurobiological correlates of these effects have been less well studied. An important site of action for estrogen in the brain is the cholinergic system. Several decades of research support the critical role of CNS cholinergic systems in cognition in humans, particularly in learning and memory formation and attention. In humans, the cholinergic system has been implicated in many aspects of cognition including the partitioning of attentional resources, working memory, inhibition of irrelevant information, and improved performance on effort-demanding tasks. Studies support the hypothesis that estradiol helps to maintain aspects of attention and verbal and visual memory. Such cognitive domains are exactly those modulated by cholinergic systems and extensive basic and preclinical work over the past several decades has clearly shown that basal forebrain cholinergic systems are dependent on estradiol support for adequate functioning. This paper will review recent human studies from our laboratories and others that have extended preclinical research examining estrogen-cholinergic interactions to humans. Studies examined include estradiol and cholinergic antagonist reversal studies in normal older women, examinations of the neural representations of estrogen-cholinergic interactions using functional brain imaging, and studies of the ability of selective estrogen receptor modulators such as tamoxifen to interact with cholinergic-mediated cognitive performance. We also discuss the implications of these studies for the underlying hypotheses of cholinergic-estrogen interactions and cognitive aging, and indications for prophylactic and therapeutic potential that may exploit these effects.

show abstract

Effects of 17β-estradiol on expression of muscarinic acetylcholine receptor subtypes and estrogen receptor α in rat hippocampus

Cited by 39 publications

References 78 publications

Local Versus Systemic Influences on Uterine Vascular Reactivity During Pregnancy in the Single-Horn Gravid Rat

Local Versus Systemic Influences on Uterine Vascular Reactivity During Pregnancy in the Single-Horn Gravid Rat

Sex differences in the neurobiology of epilepsy: A preclinical perspective

Estrogen–cholinergic interactions: Implications for cognitive aging

Contact Info

Product

Resources

About