Effects and side effects of penicillin injection in huanglongbing affected grapefruit trees

Shin, Keumchul; Ascunce, Marina S.; Narouei‐Khandan, Hossein A.; Sun, Xiao; Jones, Debra; Kolawole, Oluwaseun Olawale; Goss, Erica M.; Bruggen, A.H.C. van

doi:10.1016/j.cropro.2016.08.025

Cited by 40 publications

(25 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The combination of drench and trunk injection resulted in high activity in the root and canopy of treated plants [29]. The antibiotic activity of penicillin G was detected in the root and canopy of the 2-year-old Ray Ruby grapefruit seedlings 24 h after trunk injection with 1.0 g/tree [17]. Interestingly, the activity of penicillin G against Bacillus subtilis was only observed in the canopy, but not in the roots, of the 7-year-old Ray Ruby grapefruit trees 24 h after injection with 6.0 g/tree [17].…”

Section: Stem Versus Root Deliverymentioning

confidence: 99%

Uptake, Translocation, and Stability of Oxytetracycline and Streptomycin in Citrus Plants

et al. 2019

View full text Add to dashboard Cite

Huanglongbing (HLB), or citrus greening, is the most destructive disease to the citrus industry. In Florida, it is caused by the bacterium, Candidatus Liberibacter asiaticus (CLas) and is transmitted by the Asian citrus psyllid, Diaphorina citri. Recent studies suggested that antibiotics could inhibit the growth of the CLas pathogen in planta. In the current study, we investigated the uptake and translocation of oxytetracycline and streptomycin in citrus seedlings. Oxytetracycline and streptomycin were delivered via root and stem and their level in various tissues was monitored using enzyme-linked immunosorbent assay (ELISA). Oxytetracycline and streptomycin were detected in the leaves, xylem, phloem, and root after root drench and stem delivery. High levels of antibiotics were detected in the roots after root drench, whereas high levels of antibiotics were detected in the canopy after stem delivery. The level of oxytetracycline detected in the phloem, xylem, and leaves after root drench was higher than that of streptomycin. Whereas the level of streptomycin in root was higher than that of oxytetracycline, indicating that streptomycin was bound to the xylem tissues. Oxytetracycline and streptomycin were detected in the phloem, xylem, leaves, and root tissues thirty-five days after the root incubation in 200 µg·mL−1 solution. These results demonstrated that oxytetracycline and streptomycin were relatively stable and could inhibit CLas growth for a couple of months in citrus trees. Observations reported in this study regarding the distribution and stability of oxytetracycline and streptomycin in citrus plants could be useful for designing an effective program for the control of HLB disease using antibiotics.

show abstract

Section: Stem Versus Root Deliverymentioning

confidence: 99%

Uptake, Translocation, and Stability of Oxytetracycline and Streptomycin in Citrus Plants

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Other studies reported that streptomycin and penicillin attenuated ‘ Ca . L. asiaticus’ titer and HLB symptoms [ 12 , 13 ]. Trunk injections of streptomycin, oxytetracycline hydrochloride, and penicillin were shown to be effective against HLB [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Adjuvants on Oxytetracycline Uptake upon Foliar Application in Citrus

et al. 2020

View full text Add to dashboard Cite

Recently in Florida, foliar treatments using products with the antibiotics oxytetracycline and streptomycin have been approved for the treatment of citrus Huanglongbing (HLB), which is caused by the putative bacterial pathogen ‘Candidatus Liberibacter asiaticus’. Herein, we assessed the levels of oxytetracycline and ‘Ca. L. asiaticus’ titers in citrus trees upon foliar applications with and without a variety of commercial penetrant adjuvants and upon trunk injection. The level of oxytetracycline in citrus leaves was measured using an oxytetracycline ELISA kit and ‘Ca. L. asiaticus’ titer was measured using quantitative PCR. Low levels of oxytetracycline were taken up by citrus leaves after foliar sprays of oxytetracycline in water. Addition of various adjuvants to the oxytetracycline solution showed minimal effects on its uptake by citrus leaves. The level of oxytetracycline in leaves from trunk-injected trees was higher than those treated with all foliar applications. The titer of ‘Ca. L. asiaticus’ in the midrib of leaves from trees receiving oxytetracycline by foliar application was not affected after four days and thirty days of application, whereas the titer was significantly reduced in oxytetracycline-injected trees thirty days after treatment. Investigation of citrus leaves using microscopy showed that they are covered by a thick lipidized cuticle. Perforation of citrus leaf cuticle with a laser significantly increased the uptake of oxytetracycline, decreasing the titer of ‘Ca. L. asiaticus’ in citrus leaves upon foliar application. Taken together, our findings indicate that trunk injection is more efficient than foliar spray even after the use of adjuvants. Our conclusion could help in setting useful recommendations for the application of oxytetracycline in citrus to improve tree health, minimize the amount of applied antibiotic, reduce environmental exposure, and limit off-target effects.

show abstract

“…Recent reports showed that penicillin and streptomycin can reduce Ca. L. asiaticus titers and rescue HLB-infected plants [11,12]. Other bactericides, such as ampicillin, carbenicillin, penicillin, cefalexin, rifampicin, and sulfadimethoxine, were also found to be effective against Ca.…”

Section: Introductionmentioning

confidence: 99%

“…Other bactericides, such as ampicillin, carbenicillin, penicillin, cefalexin, rifampicin, and sulfadimethoxine, were also found to be effective against Ca. L. asiaticus [10,12,13].…”

Section: Introductionmentioning

confidence: 99%

Tracing Penicillin Movement in Citrus Plants Using Fluorescence-Labeled Penicillin

et al. 2019

View full text Add to dashboard Cite

Huánglóngbìng (HLB), citrus greening, is one of the most destructive diseases of citrus plants worldwide. In North America, HLB is caused by the phloem-limited bacterium Candidatus Liberibacter asiaticus and is transmitted by the Asian citrus psyllid, Diaphorina citri. No cure exists at present, and the use of antibiotics for the control of HLB has gained interest due to the significant losses to the citrus industry. Because of unsatisfactory results when using foliar applications of antibiotics, concerns were raised regarding the uptake and translocation of these materials within trees. We, therefore, investigated a method that allows us to study the movement of antibiotic materials in citrus plants. Herein, we utilized a fluorescence-labeled penicillin, BOCILLIN™ FL-Penicillin (FL-penicillin), to study the uptake and translocation of penicillin in citrus plants. FL-penicillin was applied by puncture to the stem of young citrus seedlings and was traced by using fluorescence microscopy. After application, we detected FL-penicillin in the leaves and in the stem xylem and phloem tissues above and below the application site in both intact and partially bark-girdled citrus seedlings, indicating that it is easily taken up and transported through the plant vascular system. In addition, we detected FL-penicillin in the gut of D. citri, which were allowed to feed on the treated plants, suggesting translocation of this molecule into the vascular tissue. We propose that the use of fluorescent-labeled molecules could be an effective tool for understanding the uptake and translocation of antibiotics and other macromolecules in plants and insects.

show abstract

Effects and side effects of penicillin injection in huanglongbing affected grapefruit trees

Cited by 40 publications

References 45 publications

Uptake, Translocation, and Stability of Oxytetracycline and Streptomycin in Citrus Plants

Uptake, Translocation, and Stability of Oxytetracycline and Streptomycin in Citrus Plants

Effect of Adjuvants on Oxytetracycline Uptake upon Foliar Application in Citrus

Tracing Penicillin Movement in Citrus Plants Using Fluorescence-Labeled Penicillin

Contact Info

Product

Resources

About